DigitalUM :: Browsing by Subject "Immunocytochemistry"

Browsing by Subject "Immunocytochemistry"

Now showing 1 - 20 of 36

Open Access
An immunocytochemical method for assaying oestrogen receptors in breast cancers. A comparison with the steroid binding assay
(Murcia : F. Hernández, 1988) Ottestad, Lars; Tveit, Kjell M.; Nesland, Jahn M.; Dybsjordl, Indrejit; Bormer, Ole; Hoie, Johan; Pihl, Alexander
The presence of oestrogen receptors was studied in 105 human breast carcinomas using monoclonal antibodies (Abbott ER-ICA kit). The oestrogen receptors of neoplastic cells were semiquantitatively measured and correlations were made to receptor values determined by a dextran-coated charcoal (DCC) steroid binding assay and to histological grade. Immunoreactive cells were found in about 213 of the tumours. Usually only a fraction of the cells within each tumour were immunoreactive, and the staining intensity varied among different cells. In general, well differentiated tumours had a greater proportion of immunoreactive cells than poorly differentiated ones. In most cases (651 98) a good agreement was found between the ER-ICA method and the DCC assay. However, in 33 cases discrepancies were demonstrated.
Open Access
Atrial natriuretic peptide in the heart and pancreas
(Murcia : F. Hernández, 1986) Lindop, G.B.M.; Mallon, E.A.; MacIntyre, G.
We used antisera to pure atrial natriuretic peptide to localise this peptide by immunocytochemistry in rat and human tissue. We showed that both rat and human atrial cardiocytes gave a positive reaction while ventricular cardiocytes were consistently negative. Peripheral islet cells in rat but not in human pancreas also showed positive staining for ANP. We showed by double labelling techniques that the ANP was present in the glucagon containing cells.
Open Access
Cellular localization of fibroblast growth factor 2 , FGF,-2 in benign prostatic hyperplasia
(Murcia : F. Hernández, 2000) Sinowatz, F.; Schams, D.; Einspanier, R.; Arnold, G.; Pfeffel, M.; Temmim-Bakers, L.; Amselgruber, W.; Plendl, J.
Fibroblast growth factor 2 (FGF-2, basic fibroblast growth factor) has been reported to be elevated in tissues from benign prostatic hyperplasia (BPH), the most frequent neoplastic disease in aging men. This suggests that FGF-2 may play a significant role in the development of BPH. In this study the cellular distribution pattern of FGF-2 in tissues from BPH has been investigated by immunohistochemical and molecular biological methods. Radioimmunoassay revealed high concentrations of FGF-2, ranging between 450 and 950 ng per g tissue. Immunoblots confirmed the presence of a 18 kDa FGF-2 in tissue extracts. By immunohistochemistry done with a polyclonal antibody to recombinant FGF-2 on paraffin sections, FGF-2 was localized in fibroblasts, endothelial cells and smooth muscle cells of tissue samples of BPH. Nuclei of these cells were labelled distinctly. Moreover the cytoplasm of smooth muscle cells was labelled moderately. No immunostaining was seen in prostatic epithelium. Nonradioactive in situ hybridization with digoxygeninlabelled oligonucleotides revealed the presence of mRNA for FGF-2 in smooth muscle cells of the prostatic stroma. These results provide evidence that FGF-2 may be produced locally in the human prostate as a stromaspecific mitogen and may play a causal role in the development of BPH.
Open Access
Central projections from the goldfish pineal organ traced by HRP-immunocytochemistry
(F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 1995) Jiménez, A.; Fenández Llebrez, P.; Pérez Fígares, J. M.
Pineal efferent projections have been traced in the brain of the goldfish (Carassius auratus) by administration of a concentrated solution of horseradish peroxidase onto the pineal organ. After different survival times, fish were sacrificed and the administered peroxidase was revealed by immunocytochemistry on paraffin sections using an anti-horseradish peroxidase antiserum. Immunoreactive fibres were seen in the anterior hypothalamus, habenula, dorsal thalamus, ventral thalamus, optic tectum, torus longitudinalis, area pretectalis, torus semicircularis and dorsal tegmentum. No immunoreactive cell bodies were visualized in the central nervous system, thus suggesting the absence of central pinealopetal innervation. Since all areas showing pineal labelled fibres are also known to receive retinal inputs, it can be suggested that an overlapping of information from retinal and extraretinal photoreceptors may be important to processes depending on photic stimulation such as entrainment of circadian rhythms or photoneuroendocrine responses.
Open Access
Characterization by immunocytochemistry of ionic channels in Helix aspersa suboesophageal brain ganglia neurons
(Murcia : F. Hernández, 2008) Azanza, M.J.; Pérez-Castejon, C.; Pes, N.; Pérez-Bruzón, R.N.; Aisa, J.; Junquera, C.; Maestú, C.; Lahoz, M.; Martínez Ciriano, M.C.; Vera Gil, A.; Del Moral, A.
The aim of this work was to characterize several ionic channels in nervous cells of the suboesophageal visceral, left and right parietal, and left and right pleural brain ganglia complex of the snail Helix aspersa by immunocytochemistry. We have studied the immunostaining reaction for a wide panel of eleven polyclonal antibodies raised against mammal antigens as follows: voltage-gated-Na+ channel; voltagegated- delayed-rectifier-K+ channel; SK2-smallconductance- Ca2+-dependent-K+ channel apamin sensitive; SK3 potassium channel; charybdotoxinsensitive voltage-dependent potassium channel; BKCamaxi- conductance-Ca2+-dependent-K+ channel; hyperpolarization-activated cyclic nucleotide-gated potassium channel 4; G-protein-activated inwardly rectifying potassium channel GIRK2 and voltage-gatedcalcium of L, N and P/Q type channels. Our results show positive reaction in neurons, but neither in glia cells nor in processes in the Helix suboesophageal ganglia. Our results suggest the occurrence of molecules in Helix neurons sharing antigenic determinants with mammal ionic channels. The reaction density and distribution of immunoreactive staining within neurons is specific for each one of the antisera tested. The studies of colocalization of immunoreaction, on alternate serial sections of the anterior right parietal ganglion, have shown for several recognized mapped neurons that they can simultaneously be expressed among two and seven different ionic protein channels. These results are considered a key structural support for the interpretation of Helix aspersa neuron electrophysiological activity.
Open Access
Chemocytoarchitecture of the rat locus ceruleus
(Murcia : F. Hernández, 1993) Lijima, K.
I shall elaborate on the cytochemical classifications of the rat locus ceruleus (LC) and state how each of these could be identified. In the LC, 80% ( 1,30911,642) of the noradrenergic (NA) neurons are also GABAergic. This is found by demonstrating that two immunoreactivities coexist in adjacent sections alternately immunostained by anti-TH or anti-GABA antibody. Pharmacological manipulations with pargyline (75 mglkg, i.p., 2 h prior to perfusion) and L-tryptophan (1 50 mglkg, 1.5 h prior to perfusion) revealed 5-HT-like immunoreactivity (5-HT-LI) in most LC cells (masked 5-HT cells) that produce 5-HT but not other indoleamines. If 5-HTP is applied instead of tryptophan, 5-HT-L1 is shown by the raphe nuclei and a few LC cells (masked indoleamine cells) in the marginal zone. Anti- GAD antibody reveals GAD-L1 in 32% of GABA neurons predominantly in the dorsal division. In situ hybridization studies detected tryptophan hydroxylase mRNA and GAD mRNA in many small- and mediumsized neurons. It is concluded that the LC consists mostly of an NA population that is possibly synthesizing multiple transmitters, such as GABA, GAD and 5-HT in single neurons so that the system enables the LC simultaneously to innervate the entire CNS.
Open Access
Detection of platelet-derived growth factor-alpha (PDGF-A) protein in cells of Leydig lineage in the postnatal rat testis
(Murcia : F. Hernández, 2006) Fecteau, K.A.; Mrkonjich, L.; Mason, J.I.; Mendis-Handagama, S.M.L.C.
Platelet-derived growth factor-A (PDGF-A) is a locally produced growth factor in the rat testis secreted by both Sertoli cells and Leydig cells. It has been suggested that PDGF-A may be involved in modulation of testosterone production and may be essential to Leydig cell differentiation, however it is not known at what stage of differentiation PDGF-A begins to be expressed in the cells of Leydig lineage in the postnatal rat testis. Therefore, the objectives of this research were to determine at what postnatal age and in which cell type is PDGF-A first expressed in cells of the adult Leydig cell lineage, and does PDGF-A expression coincide with expression of 3ß-hydroxysteroid dehydrogenase (3ß-HSD), an indicator of steroid hormone synthesis. Male Sprague Dawley rats of postnatal day 1, 7, 9-14, 21, 28, 40, 60, and 90 were used (n=6). Animals were euthanized and their testicles removed, fixed in Bouin’s solution, embedded in paraffin, and 5 µ m sections were prepared. Immunolocalization of PDGF-A and 3ß-HSD was carried out using a peroxidase-streptavidin-biotin method. PDGF-A was first detected in cells of the Leydig cell lineage at postnatal day 10 in progenitor cells, which were surrounding the seminiferous tubules (peritubular). These cells were confirmed to be the progenitor cells and not the mesenchymal or any other spindle-shaped cells in the testis interstitium by immunolocalization of 3ß-HSD and PDGF-A in the cells in adjacent sections of testis tissue from rats of postnatal days 10-14. After postnatal day 10, PDGF-A was continued to be expressed in subsequent cells of the Leydig lineage through day 90 (adult), however, was not present in peritubular mesenchymal precursor cells of the Leydig cell lineage or any other spindle-shaped cells in the testis interstitium at any tested age. These results revealed that PDGF-A first appears in Leydig progenitor cells in the postnatal rat testis at the onset of mesenchymal cell differentiation into progenitor cells at postnatal day 10 and suggest that a functional role(s) of PDGF-A in postnatally differentiated Leydig cells in the rat testis is established at the time of the onset of postnatal Leydig stem cell differentiation. It is suggested that the significance of the first expression of PDGF-A in the Leydig progenitor cells may be associated with inducing cell proliferation and migration of this cell away from the peritubular region during Leydig cell differentiation.
Open Access
Distribution of neuropeptide Y-like immunoreactivity in the brain of Salmo salar and Gambusia affinis
(Murcia : F. Hernández, 1992) García-Fernández, J.M.; Del Brío, M.A.; Cernuda-Cernuda, R.; Coto, A.; Riera, P.
Through the imrnunohistochernical PAP technique, the distribution of irnrnune positive neurons and fibres for an antibody anti-NPY in the encephalon of salrnon fixed in Bouin have been located and studied. NPY-positive neurons are found forming three important nuclei: in the ventrolateral telencephalon; in the tegmentum mesencephali; and in the locus coemleus. Neurons in the optic tecturn, in the thalarnic region and a few in the preoptic recess have also been located. The fibres were found throughout the brain, with the exception of the cerebellurn, presenting a greater density in three regions: in the dorsal telencephalon; in the mesencephalon; and in the visceral lobes in the rhombencephalon. With the airn of proving if this distribution is found in other groups of teleosts, we processed, with the sarne technique, the advanced teleost Gambusia affinis, in order to compare it with the prirnitive teleost Salmo salar. The results show that in both fish this neuropeptide has the same pattem of distribution. The results also suggest that in fish this neuropeptide can be involved in severa1 functions of the central nervous systern, as has been dernonstrated for rnarnmals. The innervation of the visceral lobes and also the presence of NPY-fibres in the posterior hypothalarnus are anatornical supports of the studies which suggest that NPY is related to the control of the food intake.
Open Access
Freeze-fracture cytochemistry,a new fracture-labeling method for topological analysis of biomembrane molecules
(Murcia : F. Hernández, 2000) Takizawa, T.; Robinson, J.M.
Freeze-fracture cytochemistry allows visualization of cellular and molecular characteristics of biomembranes in situ. In this review, we discuss freezefracture cytochemistry with special reference to a new cytochemical labeling of replicas, the detergentdigestion fracture-labeling technique. In this procedure, unfixed cells are rapidly-frozen, freeze-fractured, and physically stabilized by evaporated platinum/carbon. The frozen cells are then removed from the freezefracture apparatus to thaw and are subsequently treated with detergents. After detergent-digestion, replicas are labeled with cytochemical markers. We demonstrate that the technique is a versatile tool for direct analysis of the macromolecular architecture of biomembranes and allows identification of particular intracellular membrane organelles. In addition, we demonstrate the application of ultrasmall gold to freeze-fracture immunocytochemistry. Freeze-fracture cytochemistry is a valuable technique for investigating topology and dynamics of membrane molecules.
Open Access
‘Green Mice’ display limitations in enhanced green fluorescent protein expression in retina and optic nerve cells
(F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 2014) Caminos, Elena; Vaquero, Cecilia F.; García-Olmo, Dolores C.
Characterization of retinal cells, cell transplants and gene therapies may be helped by prelabeled retinal cells, such as those transfected with vectors for green fluorescent protein expression. The aim of this study was to analyze retinal cells and optic nerve components from transgenic green mice (GM) with the ‘enhanced’ green fluorescent protein (EGFP) gene under the control of the CAG promoter (a chicken β-actin promoter and a cytomegalovirus enhancer). The structural analysis and electroretinography recordings showed a normal, healthy retina. Surprisingly, EGFP expression was not ubiquitously located in the retina and optic nerve. Epithelial cells, photoreceptors and bipolar cells presented high green fluorescence levels. In contrast, horizontal cells, specific amacrine cells and ganglion cells exhibited a null EGFP expression level. The synaptic terminals of rod bipolar cells displayed a high green fluorescence level when animals were kept in the dark. Immature retinas exhibited different EGFP expression patterns to those noted in adults. Axons and glial cells in the optic nerve revealed a specific regional EGFP expression pattern, which correlated with the presence of myelin. These results suggest that EGFP expression might be related to the activity of both the CAG promoter and β-actin in mature retinal neurons and oligodendrocytes. Moreover, EGFP expression might be regulated by light in both immature and adult animals. Since GM are used in numerous retina bioassays, it is essential to know the differential EGFP expression in order to select cells of interest for each study.
Open Access
Hirschsprung's disease - immunohistochemical findings
(Murcia : F. Hernández, 1994) Larsson, L.T.
Hirschsprung's disease (HSCR) is characterized by a non-propulsive distal intestinal segment (usually colon) leading to a functional obstruction. An absence of ganglia in the affected - - segment explains the synonymous term ccaganglionosis coli,,. The lack of peristalsis is partly due to a deficient intestinal smooth muscle relaxation based on an absence of non-adrenergic, non-cholinergic (NANC) inhibitory innervation. Morphological studies using conventional microscopy, imrnunohistochemistry and immunochemistry against general neuronal markers and neuropeptides have been used to characterize the disturbed NANC innervation in HSCR. An increased cholinergic and adrenergic innervation is registered in the aganglionic segment in spite of the lack of neuronal cell bodies: Neuropeptides like vasoactive intestinal peptide (VIP), pituitary adenylate cyclase-activating polypeptide (PACAP), gastrin-releasing peptide (GRP), calcitonin generelated peptide (CGRP), substance P (SP), enkephalins and galanin imrnunoreactive nerve fibres are all reduced in number i n the aganglionic segment. In contrast, neuropeptide Y (NPY)-containing nerve fibres are increased in number in the diseased segment, probably reflecting the adrenergic hyperinnervation. General neuronal markers including chromogranins have been used to map the ne~lron~l network in the HSCR intestine and also to investigate the endocrine cell system in the intestinal mucosa. Nitric oxide is a potent component of the NANC inhibitory innervation and its synthesizing enzyme, nitric oxide synthase (NOS). is shown to be almost absent in the neuronal system in aganglionic intestine.
Open Access
Human lung fructose-1,6-bisphosphatase is localized in pneumocytes II
(Murcia : F. Hernández, 2001) Gizak, A.; Rakus, D.; Kolodziej, J.; Zabel, M.; Ogorzalek, A.; Dzugaj, A.
The localization of fructose-1,6-bisphosphatase (Fru-1,6-Pase EC 3.1.3.11) in human alveolar epithelium was determined immunohistochemically using a polyclonal antibody raised against the enzyme purified from human liver. The immunohistochemical analysis revealed that the Fru-1,6-Pase was localized in pneumocytes 11 and was absent in pneumocytes 1. Hypothetically Fru-1,6-Pase participating in glucose-6-phosphate synthesis from noncarbohydrate precursors increases NADPH leve1 which is used for surfactant synthesis and for glutathione redox cycle.
Open Access
IBL-like T cell lymphoma expressing monoclonal gammopathy (macroglobulinemia) in the serum
(Murcia : F. Hernández, 1989) Kasajima, Takeshi; Masuda, Akihiro; Matsuda, Mikio; Imai, Yutaka; Tobinai, Kensei
A case of IBL-like T cell lymphoma with serum monoclonal gammopathy was reported. A 58- year-old woman, who had suffered from heart failure. was admitted because of asthma attack, fever and lyrnphadenopathy. Leucopenia with a small amount of atypical lymphocytes was detected. Serum analysis showed monoclonal elevation of IgM-K (M-protein) and hv~erviscositv. Urinarv Bence-Jones urotein was diiected. Lymph &de biopsy rAealed the disappearance of normal structure and proliferation of T cells with pale cells which characterized IBL-like T cell lymphoma. Immunocytochemistry revealed the pale cells to bear T cell markers (MT-1, CD 5, CD 8 or CD 4) and IgM-positive cell distribution. Tonsilar biopsy showed the infiltration of atypical lymphoids and pale cells. Bone marrow biopsy showed moderate lymphoplasmacytoid proliferation with lymph follicles. Clinical data and serum analysis suggested macroglobulinemia. Additional lymph node biopsy was performed and revealed IBL-like T cell lymphoma. IBLlike T cell lymphoma is characterized by polyclonal hypergammaglobulinemia. The present case probably occurred initially as IBL-like T cell lymphoma and lymphoplasmacytoid cell proliferation might have followed due to an excess of CD 4' cells
Open Access
Immunocytochemical localization of the calcium-binding proteins calbindin D28K, calretinin and parvalbumin in bat visual cortex
(Universidad de Murcia. Departamento de Biología Celular e Histología, 2016) Kim, Hang-Gu; Gu, Ya-Nan; Lee, Kyoung-Pil; Lee, Ji-Gun; Kim, Chan-Wook; Lee, Ji-Won; Jeong, Tae-Hee; Jeong, Young-Wun; Jeon, Chang-Jin
It is a common misconception that bats are blind, and various studies have suggested that bats have visual abilities. The purpose of this study was to investigate the cytoarchitecture of calbindin D28K (CB)-, calretinin (CR)-, and parvalbumin (PV)-immunoreactive (IR) neurons in the bat visual cortex using immunocytochemistry. The highest density of CB- and PV-IR neurons was located in layer IV of the visual cortex. The majority of CB- and PV-IR neurons were characterized by a stellate or round/oval shape. CR-IR neurons were predominantly located in layers II/III, and the cells were principally round/oval in shape. Two-color immunofluorescence revealed that 65.96%, 24.24%, and 77.00% of the CB-, CR-, and PV-IR neurons, respectively, contained gamma-aminobutyric acid (GABA). We observed calcium-binding protein (CBP)- IR neurons in specific layers of the bat visual cortex and in specific cell types. Many of the CBP-IR neurons were GABAergic interneurons. These data provide useful clues to aid in understanding the functional aspects of the bat visual system.
Open Access
Immunocytochemical studies on prolactin cells in the adenohypophysis of the golden hamster
(Murcia : F. Hernández, 1987) Seu-Mei Wang; Chia-Man Lue; Huai-San Lin
Mammotrophs or prolactin (PRL) cells were identifed in the adenohypophysis of adult golden hamsters by immunocytochemical techniques with a polyclonal anti-PRL, that was proved to be specific to PRL by the dot immunoblotting test. Postembedding immunostaining was performed on Araldite thin sections by immunoperoxidase and immunogold methods. PRL cells were classified into three types according to the different size of the secretory granules. The Type A cells were usually small and angular or oval in shape, and had secretory granules ranging in diameter from 100-230 nm, and showed poorly developed organelles. The Type B and C cells were larger and round or ovoid in shape, contained larger granules, 230-280 nm and 280-570 nm, respectively, and displayed well developed organelles. Immunoreactive PRL cells in the male pituitaries were far less numerous than in the nonpregnant female glands, and were mostly of the Type A and B, whereas in the female the Type C and B cells predominated. In pregnant females, Type C cells became activated and increased in number, while the other two types decreased in proportion. In lactating females, Type A and B cells significantly increased in number at the expense of the Type C cells; meanwhile, the exocytosis of secretory granules was frequently found in all types of PRL cells. The present findings suggest that Type C and B PRL cells, especially the former, are potent in producing and releasing PRL and highly responsive to various physiological stimuli, while Type A cells are probably relatively inert in synthetic activity.
Open Access
Immunoelectron microscopy of human retroviruses
(Murcia : F. Hernández, 1989) Timár, J.; Lapis, K.
The ultrastructural detection and identification of human retroviruses - HTLV (human T-cell lymphotropic virus) and HIV (human immunodeficiency virus) - have become an everyday task for pathologists and virologists as well as for cell and molecular biologists. The development of better and conventionally available immunocytochemical techniques, such as preor postembedding immunocytochemical methods, cryofixation-variants and low temperature embeddings, have made it possible to use them in this field. With the help of these methods the structural proteins of HTLV-1 and HIV have been identified in infected cells. The virus assembly at the cell membrane has also been described in detail. Using these methods the incorporation of human transplantation antigens into the envelope of these viruses can be followed. Future studies should establish the pathological significance of this process.
Open Access
Immunohisto(cyto)chemistry: an old time classic tool driving modern oncological therapies
(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Cooks, Tomer; Theodorou, Sofia D.P.; Paparouna, Eleni; Rizou, Sophia V.; Myrianthopoulos, Vassilios; Gorgoulis, Vassilis G.; Pateras, Ioannis S.
In the era of precision medicine immunohistochemistry (IHC) and immunocytochemistry (ICC) share some of the highlights in personalized treatment. Survival data obtained from clinical trials shape the cut-offs and IHC scoring that serve as recommendations for patient selection both for targeted and conventional therapies. Assessment of Estrogen and Progesterone Receptors along with HER2 status has been among the first approved immunostaining assays revolutionizing breast cancer treatment. Similarly, ALK positivity predicts the efficacy of ALK inhibitors in patients with non-small cell lung cancer (NSCLC). In recent years, Programmed Death Ligand 1 (PD-L1) IHC assays have been approved as companion or complimentary diagnostic tools predicting the response to checkpoint inhibitors. Anti-PD-L1 and anti-PD-1 monoclonal antibodies have inaugurated a new period in the treatment of advanced cancers, but the path to approval of these biomarkers is filled with immunohistochemical challenges. The latter brings to the fore the significance of molecular pathology as a hub between basic and clinical research. Besides, novel markers are translated into routine practice, suggesting that we are at the beginning of a new exciting period. Unraveling the molecular mechanisms involved in cellular homeostasis unfolds biomarkers with greater specificity and sensitivity. The introduction of GL13 (SenTraGor®) for the detection of senescent cells in archival material, the implementation of key players of stress response pathways and the development of compounds detecting common mutant P53 isoforms in dictating oncological treatments are paradigms for precision oncology.
Open Access
Iron-binding proteins in human colorectal adenomas and carcinomas: an immunocytochemical investigation
(Murcia : F. Hernández, 1992) Tuccari, G.; Rizzo, A.; Crisafulli, C.; Barresi, G.
By immunocytochemistry, the presence of major iron-binding proteins (lactoferrin, transferrin and ferritin) was investigated in tubular adenomas (12 cases), villous adenomas (7 cases), carcinomas of the large bowel and rectum (39 cases) and lymph nodes involved in carcinomas (8 cases); 5 samples of colonic inflarnrnatory pseudopolyps were aiso studied. Dysplastic areas of tubular and villous adenomas as well as adenocarcinomas and colloid carcinomas showed a variable cytoplasmic immunoreactivity for al1 antisera, although no staining was noted in some cases; tubular adenomas without dysplasia and colonic inflarnmatory pseudopolyps were always unstained. Metastatic elements present in lymph nodes maintained the irnmunohistochemicai staining for iron-binding proteins. An autoctone production of lactoferrin, transferrin and ferritin by tumour cells may be hypothesized in relation to the increased requirement of iron for the tumover of rapidly dividing cells.
Open Access
lbotenic acid-induced lesions of striatal target and projection neurons ultrastructural manifestations in dopaminergic and non-dopaminergic neurons and in glia
(Murcia : F. Hernández, 1987) Smith, Diane E.; Makoto Saji; Joh, Tong H.; Reis, Donald J.; Pickel, Virginia M.
Open Access
lmmunocytochemical distribution of serotonin and neuropeptide Y, NPY in mouse adrenal gland
(Murcia : F. Hernández, 1993) Fernandez-Vivero, J.; Rodríguez Sánchez, F.; Verastegui, C.; Cordoba Moriano, F.; Romero, A.; De Castro, J.M.
By the use of imrnunocytochetnicd staining methods. we studied the morphology and distribution of SHT and NPY immunoreactive cells and fibres in the mouse adrenal gland. The 5HT-immunoreactive cells were numerous and widely localized in the medullar tissue. These cells were arranged in three cellular types with regard to their morphological and immunocytochemical features. One of them showed cells with polygonal shape, being intensified like the typical medullary chromaffin cells. These imniunoreactive cells were observed arranged in medullar islets. The second SHT-inimi~noreactive celular type was constituted by cells with polygonal shape and strong immunoreactivity. The third one was formed by cells with irnmunoreactive prolongations. We found some islets of chromaffin nonirnmunoreactive cells surrounded by immunostained cells. We also observed some 5HT-imniunoreactive nerve fibres in the rnedullar tissue. NPY-like itlitnunoreactivity was detected in both chromaffin and ganglion cells in adrenal medulla. NPY-like imrnunoreactivity was also detected in nerve fibres at cortical level. In a few cases, we observed medullar SHT- and NPYinimunoreactive tissue in the adrenal cortex (monotremas).