Publication: Chemocytoarchitecture of the rat locus ceruleus
Authors
Lijima, K.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
I shall elaborate on the cytochemical
classifications of the rat locus ceruleus (LC) and state
how each of these could be identified. In the LC, 80%
( 1,30911,642) of the noradrenergic (NA) neurons are also
GABAergic. This is found by demonstrating that two
immunoreactivities coexist in adjacent sections
alternately immunostained by anti-TH or anti-GABA
antibody. Pharmacological manipulations with pargyline
(75 mglkg, i.p., 2 h prior to perfusion) and L-tryptophan
(1 50 mglkg, 1.5 h prior to perfusion) revealed 5-HT-like
immunoreactivity (5-HT-LI) in most LC cells (masked
5-HT cells) that produce 5-HT but not other
indoleamines. If 5-HTP is applied instead of tryptophan,
5-HT-L1 is shown by the raphe nuclei and a few LC cells
(masked indoleamine cells) in the marginal zone. Anti-
GAD antibody reveals GAD-L1 in 32% of GABA
neurons predominantly in the dorsal division. In situ
hybridization studies detected tryptophan hydroxylase
mRNA and GAD mRNA in many small- and mediumsized
neurons. It is concluded that the LC consists
mostly of an NA population that is possibly synthesizing
multiple transmitters, such as GABA, GAD and 5-HT in
single neurons so that the system enables the LC
simultaneously to innervate the entire CNS.
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