Publication: Morphological characterisation of BGM (Buffalo Green Monkey) cell line exposed to low doses of cadmium chloride
Authors
Gómez Zapata, M. ; García Fernández, Antonio Juan ; Luna Maldonado, Aurelio ; Romero García, Diego
item.page.secondaryauthor
item.page.director
Publisher
Elsevier
publication.page.editor
publication.page.department
DOI
http://dx.doi.org/10.1016/S0887-2333(03)00017-1
item.page.type
info:eu-repo/semantics/article
Description
©2003. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted, version of a Published Work that appeared in final form in Toxicology in Vitro (TIV). To access the final edited and published work see http://dx.doi.org/10.1016/S0887-2333(03)00017-1
Abstract
Morphological changes in the Buffalo Green Monkey (BGM) cell line after exposure to a subcytotoxic dose (0.062 mm, equivalent to EC10—effective concentration 10%) of cadmium chloride have been evaluated. Cells were exposed for 24 h and the effects observed at the ultrastructural level by transmission and scanning microscopy. Using transmission electron microscopy, the most notable findings in treated cells were the presence of intranuclear inclusion bodies and thin intracytoplasmic granules associated to myelin figures and the presence of apoptotic bodies. Other morphological alterations included cell vacuolisation and a reduced
cytoplasm volume, condensation of the mitochondria and a decreased number of cytoplasmic organelles, except lysosomes and autophagic vacuoles, which increased in number. Scanning electron microscopy pointed to a cell with a disrupted perinuclear region and a decrease in the number of surface microvilli. We conclude that the BGM cell line may be considered an useful tool for toxicological studies involving cadmium.
publication.page.subject
Citation
Toxicology in Vitro 17 (2003) 293–299
item.page.embargo
Collections
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/