Morphological characterisation of BGM (Buffalo Green Monkey) cell line exposed to low doses of cadmium chloride

Romero García, Diego; Gómez Zapata, M.; Luna, A.; García Fernández, A.J.

Publication:
Morphological characterisation of BGM (Buffalo Green Monkey) cell line exposed to low doses of cadmium chloride

dc.contributor.author	Romero García, Diego
dc.contributor.author	Gómez Zapata, M.
dc.contributor.author	Luna, A.
dc.contributor.author	García Fernández, A.J.
dc.contributor.department	Ciencias Sociosanitarias
dc.date.accessioned	2024-03-11T12:39:03Z
dc.date.available	2024-03-11T12:39:03Z
dc.date.issued	2003-01-23
dc.description	©2003. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Toxicology in Vitro (TIV). To access the final edited and published work see http://dx.doi.org/10.1016/S0887-2333(03)00017-1	es
dc.description.abstract	Morphological changes in the Buffalo Green Monkey (BGM) cell line after exposure to a subcytotoxic dose (0.062 mm, equivalent to EC10—effective concentration 10%) of cadmium chloride have been evaluated. Cells were exposed for 24 h and the effects observed at the ultrastructural level by transmission and scanning microscopy. Using transmission electron microscopy, the most notable findings in treated cells were the presence of intranuclear inclusion bodies and thin intracytoplasmic granules associated to myelin figures and the presence of apoptotic bodies. Other morphological alterations included cell vacuolisation and a reduced cytoplasm volume, condensation of the mitochondria and a decreased number of cytoplasmic organelles, except lysosomes and autophagic vacuoles, which increased in number. Scanning electron microscopy pointed to a cell with a disrupted perinuclear region and a decrease in the number of surface microvilli. We conclude that the BGM cell line may be considered an useful tool for toxicological studies involving cadmium.	es
dc.format	application/pdf	es
dc.format.extent	7	es
dc.identifier.citation	Toxicology in Vitro 17 (2003) 293–299
dc.identifier.doi	http://dx.doi.org/10.1016/S0887-2333(03)00017-1
dc.identifier.issn	Print: 0887-2333
dc.identifier.uri	http://hdl.handle.net/10201/140096
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	This work was supported by CICYT, project AGF97-526	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	BGM	es
dc.subject	Cytotoxicity	es
dc.subject	Cadmium	es
dc.subject	Apoptosis	es
dc.subject	Ultrastructural alterations	es
dc.subject.other	CDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca	es
dc.title	Morphological characterisation of BGM (Buffalo Green Monkey) cell line exposed to low doses of cadmium chloride	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es