Browsing by Subject "BGM"

Now showing 1 - 3 of 3
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    A comparison of BGM and LLC-PK1 cells for the evaluation of nephrotoxicity
    (Taylor and Francis Group, 2011-09-22) Tagliati, Carlos A.; Romero García, Diego; Dutra, Elisabete C.R.; Hernández-García, Alejandro; Martínez-López, Emma; García-Fernández, Antonio J.; Ciencias Sociosanitarias
    Nephrotoxicity is one of the most frequent effects observed after the use of medicine. Such situations have been tardily discovered because of existing methods to determine toxicity. The validation of sensitive, alternative methods for the early identification of toxic effects is as important as restrictions on the use of animals. In this light, the present study evaluated the effects of gentamicin on BGM and LLC-PK1 cells, using MTT and Neutral Red (NR). Although the LLC-PK1 cell line is used for toxicological studies, the BGM cell line is relatively new for this purpose. MTT (BGM: EC50=6.29mM; LLC-PK1: EC50=8.01mM) was found to be more sensitive than NR (EC50 was greater than 10mM for both cells). By using MTT, both cells demonstrated the involvement of mitochondria in a manner that was dose dependent, with an apoptotic process occurring at the concentrations of 1 and 3mM and necrosis at concentrations above 4mM. It could, therefore, be concluded that 1) BGM appears to be useful in the study of the mechanism of nephrotoxicity caused by gentamicin and 2) because of its sensitivity to MTT, in addition to its ease of manipulation, it is believed that the BGM cell line can also be used as an alternative method to evaluate nephrotoxicity.
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    Morphological characterisation of BGM (Buffalo Green Monkey) cell line exposed to low doses of cadmium chloride
    (Elsevier, 2003-01-23) Romero García, Diego; Gómez Zapata, M.; Luna, A.; García Fernández, A.J.; Ciencias Sociosanitarias
    Morphological changes in the Buffalo Green Monkey (BGM) cell line after exposure to a subcytotoxic dose (0.062 mm, equivalent to EC10—effective concentration 10%) of cadmium chloride have been evaluated. Cells were exposed for 24 h and the effects observed at the ultrastructural level by transmission and scanning microscopy. Using transmission electron microscopy, the most notable findings in treated cells were the presence of intranuclear inclusion bodies and thin intracytoplasmic granules associated to myelin figures and the presence of apoptotic bodies. Other morphological alterations included cell vacuolisation and a reduced cytoplasm volume, condensation of the mitochondria and a decreased number of cytoplasmic organelles, except lysosomes and autophagic vacuoles, which increased in number. Scanning electron microscopy pointed to a cell with a disrupted perinuclear region and a decrease in the number of surface microvilli. We conclude that the BGM cell line may be considered an useful tool for toxicological studies involving cadmium.
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    Morphological characterization of renal cell lines (BGM and VERO) exposed to low doses of lead nitrate.
    (Sociedad Española de Histología e Ingeniería Tisular, 2004) Romero García, Diego; Gómez Zapata, Maximiliano; Luna, A.; García Fernández, Antonio Juan; Ciencias Sociosanitarias
    The response to lead nitrate has been assessed in two cell lines of renal origin. The range of toxic concentrations was determined by Neutral Red assay after 24-h of exposure. Morphological changes in the Buffalo Green Monkey (BGM) and VERO cell lines after exposure to subcytotoxic doses (1.38 mM and 1.04 mM, respectively) equivalent to EC10 (effective concentrations 10%) of lead nitrate were evaluated at the ultrastructural level by transmission microscopy. The most notable finding in treated cells was the presence of inclusion bodies in the form of irregular granules of varying size in both cytoplasm and lysosomes. Cell membrane integrity was not affected. The number of phagolysosomes and myeline figures associated to the inclusion bodies was higher than in the control cultures. We conclude that the phagolysosomic mechanism fails to digest this metal ion and the BGM and VERO renal cell lines can be considered as useful tools for toxicological studies involving lead nitrate.