Histology and histopathology Vol. 2, nº 3 (1987)
Permanent URI for this collection
Browse
Browsing Histology and histopathology Vol. 2, nº 3 (1987) by Issue Date
Now showing 1 - 16 of 16
Results Per Page
Sort Options
- PublicationOpen AccessCytostatic action of methylmercuric chloride on mammalian duodenal cells(Murcia : F. Hernández, 1987) Curle, D.C.; Ray, M.; Persaud, T.V.N.; Bertalanffy, F.D.Adult male mice of the ICRISwiss Webster strain received a single intragastric administration of methylmercuric chloride 1,000 ppm, at dose levels of 5,10,15,20,25 and 30 mglkg of body weight. The animals were killed six hours later. Tissue samples from the duodenum were fixed in 10% neutral buffered formalin for light microscopy. Chromosome clumping was observed in dividing cells at all dose levels, resembling a C-mitotic effect. It would lead to reduced mitotic cell formation on account of the subsequent lysis of the arrested metaphases. The cytostatic effect was brought about by the inactivation of the microtubule spindle fiber polymerization mechanism induced by methylmercuric chloride. There was a direct positive correlation between the varying dose levels of methylmercury and the proportion of cells arrested in metaphase in the crypts of the duodenum.
- PublicationOpen AccessUltrastructural study of granulomatous tissue in tonsillar malakoplakia(Murcia : F. Hernández, 1987) Vitellaro-Zuccarello, L.; Felletti, V.; Paizis, G.; Spinelli, M.
- PublicationOpen AccessScanning electron microscopy of human and murine melanoma cells exposed to medium chain-length, C6mC,2d i carboxylic acids in tissue culture(Murcia : F. Hernández, 1987) Breathnach, A. S.; Robins, E. J.; Bhasin, Y. P.; Ethridge, L. B.; Nazzaro-Porro, M.; Passi, S.; Picardo, M.Human and murine (Harding-Passey and Cloudman) melanoma cells were exposed to various concentrations (1 X 10-3M-l X 10-lM) of adipic (C,), azelaic (C,), and dodecanedioic (C,,) acids for 1-6 hours in tissue culture, and the effects on shape and surface topography were examined by scanning electron microscopy. Effects, i.e., rounding up, concentration of microvilli, blebbing, and prominence of retraction fibrils were time and dose dependent, and for the same concentrations and exposure times, C,, had a greater effect than C,, and both a significantly greater effect than C,. These differential reactions to the three diacids parallel previously reported effects on cell kinetics and viability. The changes could be due to a prime effect on the cell membrane, or they might reflect phases of the cell cycle directed by action of the diacids on the nucleus; this latter seems unlikely. An effect on the cytoskeleton is possibly involved.
- PublicationOpen AccessAn ultrastructural examination of murine alveolar macrophages following intranasal administration of Propionibacterium acnes(Murcia : F. Hernández, 1987) Hightower, James A.; Welsh, Marcia G.; Jackson, R.A.; Gangemi, J. DavidLight and electron microscopic analysis of murine lungs or isolated pulmonary cells was performed three days after intranasal administration of the bacterial immunostimulant, Propionibacterium acnes (P. acnes). Our observations indicated that pulmonary alveolar and airway macrophages (PAMs) were the only cells with P. acnes bacilli in their cytoplasm. Bacilli were not observed in pulmonary interstitial macrophages, granulocytes, lymphocytes or pulmonary parenchyma1 cells such as type I and type I1 pneumocytes. Because of the morphological heterogeneity of PAMs observed in control and experimental animals, it was not possible from these studies to be certain about the relative abundance or complexity of lysosomes, endoplasmic reticulum, Golgi and other organelles in the two groups. However, we noted that it was not uncommon to observe in the same PAM, profiles of P. acnes and a well developed Golgi complex and endoplasmic reticulum. These P. acnes - associated morphological alterations occurred at a time when functional activities (e.g., phagocytosis, cytostasis) of PAMs were enhanced.
- PublicationOpen AccessMononuclear cell subpopulations and infiltrating lymphocytes in erythema dyschromicumperstans and vitiligo(Murcia : F. Hernández, 1987) Gross, Ana; Tapla, F.J; Mosca, Walter; Perez, Ricardo M.; Briceño, Luis; Henriquez, Juan J.; Convit, J.
- PublicationOpen AccessAssociation of sinus histiocytosis with massive lymphadenopathy and idiopathic hypereosinophilic syndrome(Murcia : F. Hernández, 1987) Hernández, Dimas; Gutierrez, Luis; Duque, Herminia; Olavarria, Renato; Muci, Rafael; Wunaii, HermanA patient was evaluated because of edema, pruritus and generalized painless lymphadenopathy. Laboratory tests showed marked eosinophilia without known etiology. CT scan of abdomen revealed multiple lymph nodes in retroperitoneal area. Lymph node biopsy was reported as sinus histiocytosis, bone marrow biopsy showed hipercellularity with marked infiltration of normal eosinophils. During his admission he developed Coombs positive hemolytic anaemia. Once he was stable, a laparotomy was performed and the patient died two days later because of septic shock. Autopsy revealed sinus histiocytosis with massive lymphadenopathy (SHML) with extranodal involvement of duodenum, spleen and prostate; septic liver and spleen, pyelonephritis, marked infiltration of eosinophilis in lymph nodes, spleen, liver duodenum and lungs. To the best of our knowledge, this is the first case report of the association of SHML and Idiopathic Hypereosinophilic Sundrome (HES) .
- PublicationOpen AccessFat-rich diet induces inflammatory changes in the intact rat pancreas(Murcia : F. Hernández, 1987) Juhani Ramo, O.; Jalovaara, P.; Apaja-Sarkkinen, M.
- PublicationOpen AccessSmooth muscle distribution in the capsule and trabeculae of the caprine superficial cervical lymph node(Murcia : F. Hernández, 1987) Faroon, O. M.; Henry, R. W.; Al-Bagdadi, F.K.This study centers around the dichotomy found in the literature concerning the presence of smooth muscle cells in the trabeculae and capsule of lymph nodes. Various superficial lymph nodes (mammary, mandibular, popliteal, subiliac, and superficial cervical) of the goat were collected and examined by light and electron microscopy. Smooth muscle cells were demonstrated in the capsule and trabeculae of lymph nodes independent of the blood and lymph vessels
- PublicationOpen AccessPrimary malignant melanoma of the nasal cavity, report of two cases and review of the literature(Murcia : F. Hernández, 1987) Sidoni, A.; Ottaviani, F.; Maurici, M.Primary malignant melanomas of the nasal cavity are rare, as only 400 cases have been reported to date. The present paper describes two cases recently seen in Caucasian women. The authors point out the difficult clinical diagnosis, as the symptoms are rather aspecific. From the histopathological point of view, diagnosis is easy in the melanotic cases while can show interpretating problems in the amelanotic ones, when melanoma is almost indistinguishable from other malignant neoplasms. A correlation between histological grading and prognosis was not detected, as both cases showed local recurrences within one year after surgery although they were, respectively, of epithelioid and indifferentiated type. While surgery appears to be the choice treatment of the primary lesion, the treatment of cervical metastasis is still disputable. On the whole, most authors think that the role played by radio- and mainly chemo-therapy is still limited and that cervical adenopathies should be treated by a simple lympho-adenectomy rather than by a neck dissection.
- PublicationOpen AccessRhabdomyoma of the soft palate. Fine structural details of a highly differentiated muscle tumor(Murcia : F. Hernández, 1987) Bock, D.; Böck, P.
- PublicationOpen AccessMorphological changes in the isolated rat liver perfused in a non-recirculating system, scanning and transmission electron microscopy(Murcia : F. Hernández, 1987) Al-Ali, Saad Y.; Hassan, Ibrahim M.; Al-Zuhair, A.G.H.Isolated perfused rat livers have been used for various studies, but detailed investigation into the structural integrity of hepatocytes of this system is lacking. In this study, isolated rat livers were perfused in vitro with oxygenated Krebs-Ringer bicarbonate buffer solution, for 2 minutes and l , 2,3, and 4 hour (S) at 37"C, using a non-recirculating perfusion system. The perfused livers were processed for semithin section light microscopy, transmission electron microscopy, and scanning electron microscopy. Sectional areas of cell deaths were measured by a camera-tracing assembly from 1.5 pm thick Araldite sections stained with toluidine blue. Progressive nuclear and cytoplasmic changes, leading to cell death, occurred in the hepatocytes of the centrilobular zone, during the 2nd, 3rd, and 4th hour of the perfusion at a rate of 9.03% ?IS%, 38.7% f2.7%, and55.1% f 5.9% (mean f standard deviation) of the total sectional areas respectively. Midzonal hepatocytes showed normal basophilic staining but exhibited loss of glycogen granules. loss of microvilli, development of aqueous vacuoles and formation of blebs. The fine structures of cell organelles, glycogen granules, microvilli and plasma membrane of the cells in the periportal zone were well preserved throughout the experimental period. For further quantitative, metabolic and functional studies using isolated rat liver perfused with Krebs-Ringer solution, it is evident from the present investigation tKat the periportal zone represents the functional region of the hepatic lobule. Whilst progressive changes, leading to cell death, occurred in the centrilobular zone.
- PublicationOpen AccessCarcinosarcoma of the gallbladder(Murcia : F. Hernández, 1987) Herrera-Goepfert, Roberto; Manrique Ortega, José de Jesús; Rodríguez-Martínez, Hector AbelardoThe case of a 60-year-old woman with a fiveyear history of abdominal pain, jaundice, and weight loss is presented. On physical examination a hard mass on her right flank was evident. She died under unknown circumstances while she was waiting to be examined. At post-mortem examination the gallbladder was replaced by a neoplasm and there were gallstones within its lumen. Histologically, the tumour was constituted by a mixture of adenocarcinoma, squamous cell carcinoma and undifferentiated sarcoma. The diagnosis of carcinosarcoma of the gallbladder was established. A review of previously reported cases is presented too.
- PublicationOpen Accesslmmunohistochemical localization of collagens and fibronectin in human breast neoplasms(Murcia : F. Hernández, 1987) Al-Adnani, M. S.; Taylor, S.; Al-Bader, A.A.; Al-Zuhair, A.G.H.; McGee, James O`D.Forty four specimens from neoplastic, hyperplastic and normal human breast tissues were studied for localization of collagens and fibronectin. Affinity purified antihuman type I, I11 and IV collagens and antifibronectins were utilized by the indirect immunoperoxidase technique on fixed and paraffinembedded sections. 86% of the cell cytoplasm of infiltrating ductal and 83% of the lobular cancers were positively stained for collagen type I and 111. Collagen type IV, however, was detected in 100% of infiltrating ductal and 83% of lobular carcinomas. Focal cytoplasmic staining is a predominant feature for all antigens in the intraduct carcinoma while a diffuse pattern is encountered in the infiltrating types. Intact basement membranes in various lesions always stained for type IV collagen and showed variable staining for type I11 collagen and fibronectin. Epithelia of normal, benign, hyperplastic breast and most medullary carcinoma were negative for the three collagen types. Our results are in favour of the view that infiltrating breast carcinoma cells produce inappropriately the majority of collagens and inconsistently other proteins such as fibronectin.
- PublicationOpen AccessGlomerular histopathology of the contralateral kidney in experimental unilateral hydronephrosis(Murcia : F. Hernández, 1987) Moyano, M.C.; Gázquez, A.; Redondo, E.; Roncero, V.The aim of this study was to examine the structural, ultrastructural and morphometric alterations which take place in the contralateral kidneys of rats with experimental unilateral hydronephrosis. 20 Wistar rats weighing 250 gr., affected by a process of unilateral hydronephrosis following the ligature of the ureter, were used; these rats were then killed 40,50,60, or 70 days after the ligature. Among the perceived alterations, were immunoglobulin G deposits shown by positive immunoperoxidase reaction and increase in the size of the glomerular and corpuscle from around the fortieth day, and structural alterations that included the pedicels, electrondense deposits in the podocytes and pseudogranular structures in the basal membrane of the capillary.
- PublicationOpen AccessEffect of N-Ethyl-N-Butyl-Nitrosamine on the esophageal mucosa of the rat. Histometric investigation of early tumor stages(Murcia : F. Hernández, 1987) Sons, H.U.; Borchard, F.; Kaup, F.G.80 male Wistar rats received an oral ad libitum application of N-ethyl-N-butyl-nitrosamine in a concentration of 0.18 g per litre of drinking water. The changes induced in the esophakeal mucosa and determined at three intervals (up to 48 days, up to 91 days, and up to 112 days after commencement of carcinogen exposure) were compared by microscopy with the results from a control group of 10 male Wistar rats of the same age. Several histomorphometric parameters were investigated with the aid of a Leitz ocular micrometer. The earliest localized changes found were an increase in the thickness of the epithelium and the horny layer, and an elongation of the papillary bodies and a widening of the parabasal cellular layer. Later - with a substantial increase in the rate of mitosis in all layers of the epithelium, there was a significant thickening of the non-papillomatous and papillomatous epithelium, an enlargement of the nuclei, especialli in the middle and upper layers of the epithelium and a thickening of the horny layer, parts of the latter being parakeratotic. The papillomatous changes corresponded in some cases to moderate epithelia1 dysplasias. As expected, no fully-developed invasive carcinomas occurred in the early period investigated. The histometric data permit the conclusion to be drawn that the lesions described are demonstrable not only at the exophytic-papillomatous epithelium but also in multifocally localized form at the flat, nonpapillomatous mucosa, and that they can definetely be regarded as the expression of an incipient field cancerification
- PublicationOpen Accesslbotenic acid-induced lesions of striatal target and projection neurons ultrastructural manifestations in dopaminergic and non-dopaminergic neurons and in glia(Murcia : F. Hernández, 1987) Smith, Diane E.; Makoto Saji; Joh, Tong H.; Reis, Donald J.; Pickel, Virginia M.