Publication: Effect of N-Ethyl-N-Butyl-Nitrosamine on the esophageal mucosa of the rat. Histometric
investigation of early tumor stages
Authors
Sons, H.U. ; Borchard, F. ; Kaup, F.G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
80 male Wistar rats received an oral ad
libitum application of N-ethyl-N-butyl-nitrosamine in a
concentration of 0.18 g per litre of drinking water. The
changes induced in the esophakeal mucosa and determined
at three intervals (up to 48 days, up to 91 days, and up to
112 days after commencement of carcinogen exposure)
were compared by microscopy with the results from a
control group of 10 male Wistar rats of the same age.
Several histomorphometric parameters were investigated
with the aid of a Leitz ocular micrometer. The earliest
localized changes found were an increase in the thickness
of the epithelium and the horny layer, and an elongation
of the papillary bodies and a widening of the parabasal
cellular layer. Later - with a substantial increase in the
rate of mitosis in all layers of the epithelium, there was a
significant thickening of the non-papillomatous and
papillomatous epithelium, an enlargement of the nuclei,
especialli in the middle and upper layers of the
epithelium and a thickening of the horny layer, parts of
the latter being parakeratotic. The papillomatous
changes corresponded in some cases to moderate
epithelia1 dysplasias. As expected, no fully-developed
invasive carcinomas occurred in the early period
investigated. The histometric data permit the conclusion
to be drawn that the lesions described are demonstrable
not only at the exophytic-papillomatous epithelium but
also in multifocally localized form at the flat, nonpapillomatous
mucosa, and that they can definetely be
regarded as the expression of an incipient field cancerification
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