Publication: Scanning electron microscopy of human and murine melanoma cells exposed to medium chain-length, C6mC,2d i carboxylic acids in tissue culture
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Date
1987
Authors
Breathnach, A. S. ; Robins, E. J. ; Bhasin, Y. P. ; Ethridge, L. B. ; Nazzaro-Porro, M. ; Passi, S. ; Picardo, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Human and murine (Harding-Passey and
Cloudman) melanoma cells were exposed to various
concentrations (1 X 10-3M-l X 10-lM) of adipic (C,),
azelaic (C,), and dodecanedioic (C,,) acids for 1-6 hours
in tissue culture, and the effects on shape and surface
topography were examined by scanning electron
microscopy. Effects, i.e., rounding up, concentration of
microvilli, blebbing, and prominence of retraction fibrils
were time and dose dependent, and for the same
concentrations and exposure times, C,, had a greater
effect than C,, and both a significantly greater effect than
C,. These differential reactions to the three diacids
parallel previously reported effects on cell kinetics and
viability. The changes could be due to a prime effect on
the cell membrane, or they might reflect phases of the
cell cycle directed by action of the diacids on the nucleus;
this latter seems unlikely. An effect on the cytoskeleton
is possibly involved.
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