Publication: Evaluation of a commercial double-recognition ELISA for caprine tuberculosis diagnosis: sensitivity, specificity, and correlation to macroscopic and microscopic lesions
Authors
Salinas Lorente, Jesús ; Del Río, Laura ; Buendía Marín, Antonio Julián ; Navarro Cámara, José Antonio ; Ortega Hernández, Nieves ; Sánchez Campillo, Joaquín
item.page.secondaryauthor
item.page.director
Publisher
Elsevier
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1016/j.rvsc.2022.09.034
item.page.type
info:eu-repo/semantics/article
Description
© 2022 Elsevier Ltd. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/10.1016/j.rvsc.2022.09.034
Abstract
Ante-mortem diagnosis of caprine tuberculosis, as in other ruminant species, is a complex process. Therefore, it is sometimes necessary to apply a combination of methods that increases the sensitivity of diagnosis while maintaining adequate specificity. In the present study, we analysed the use of ELISA based on a double-recognition methodology for the diagnosis of goat tuberculosis. Serum samples from animals with tuberculosis,
confirmed by histopathological studies and presence of Ziehl-Neelsen-positive bacteria in the lesions, were used to assess sensitivity. Sera from flocks without tuberculosis were used to assess diagnostic specificity. The
relationship between ELISA results and the type of tuberculous lesions, both macroscopic and microscopic, was analysed. The sensitivity and specificity of ELISA test were 69.9% and 97.9%, respectively. Sensitivity was higher in animals with macroscopic lesions, indicating more advanced forms of the disease. Similarly, a higher sensitivity was found in animals with microscopic lesions associated with active tuberculosis, such as cavitary lesions.
publication.page.subject
Citation
item.page.embargo
Collections
Ir a Estadísticas
Sin licencia Creative Commons.