Buendía Marín, Antonio Julián

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Buendía Marín, Antonio Julián

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Buendía Marín, Antonio Julián

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Anatomía y Anatomía PatológicaComparadas

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Open Access
Prevalencia de la clamidiosis ovina y caprina en la región de murcia
(Murcia : Universidad de Murcia, Servicio de Publicaciones, 1992) Cuello Gijón, Francisco; Salinas Lorente, Jesús; Caro Vergara, María Rosa; Gallego Ruiz, María del Carmen; Sánchez-Gallego, María J.; Bretón, J.; Buendía Marín, Antonio Julián; Facultad de Veterinaria
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Characterization of a murine model of intranasal infection suitable for testing vaccines against C. abortus
(Elsevier, 2007-01-15) Nicolás, L.; Gallego, M. C.; Sánchez, J.; Caro, M. R.; Salinas, J.; Martínez Cáceres, Carlos Manuel; Navarro Cámara, José Antonio; Ortega Hernández, Nieves; Buendía Marín, Antonio Julián; Anatomía y Anatomía Patológica Comparadas
Mouse models have been widely used to test candidate vaccines against Chlamydophila abortus infection in mice. Although the induction of a systemic infection by endogenous or intraperitoneal inoculation is a useful tool for understanding the immune mechanism involved in the protection conferred by the vaccination, a different approach is necessary to understand other factors of the infection, such as mucosal immunity or the colonization of target organs. To test whether C. abortus intranasal model of infection in mice is a useful tool for testing vaccines in a first group of experiments mice, were infected intranasally with C. abortus to characterize the model of infection. When this model was used to test vaccines, two inactivated experimental vaccines, one of them adjuvated with QS-21 and another with aluminium hydroxide, and a live attenuated vaccine (strain 1B) were used. Non-vaccinated control mice died within the first 8 days, after displaying substantial loss of weight. Histologically, the mice showed lobar fibrinopurulent bronchointerstitial pneumonia. Prior immunization with QS-21 adjuvated vaccine or 1B vaccine presented mortality and the recipients showed a greater number of T cells in the lesions, especially CD8+ T cells, than the control mice and mice immunized with vaccine adjuvated with aluminium hydroxide. The results confirm that the C. abortus intranasal model of infection in mice is a useful tool for testing vaccines
Open Access
Clamidiosis porcina: estudio preliminar en la Región de Murcia
(Murcia: Universidad de Murcia, Servicio de Publicaciones, 1996) Buendía Marín, Antonio Julián; Salinas Lorente, Jesús; Cano, L. D.; Cuello Gijón, Francisco; Buendía Marín, Antonio Julián; Facultad de Veterinaria
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Effects of diabetes on oxidative stress, periodontal ligament fiber orientation and matrix metalloproteinase 8 and 9 expression during orthodontic tooth movement
(2021) Vicente, Ascensión; Bravo-González, Luis-Alberto; Camacho-Alonso, Fabio; Navarro Cámara, José Antonio; Buendía Marín, Antonio Julián; Dermatología, Estomatología, Radiología y Medicina Física
Objectives: To evaluate the influence of diabetes on oxidative stress, periodontal ligament (PDL) orientation, and matrix metalloproteinase (MMP) 8 and 9 expressions during orthodontic tooth movement in a rat model. Materials and methods: An orthodontic appliance was placed in 60 Sprague-Dawley rats divided into three groups: normoglycemics (n = 20) and two streptozotocin-induced diabetic groups, one untreated (n = 20) and one insulin-treated (n =20). At 24, 48, and 72 h and 1 week, rats were sacrificed. At each time point, myeloperoxidase (MPO) and malondialdehyde (MDA) were quantified by spectrophotometry, tooth movement was evaluated by micro-CT analysis, and hematoxylin and eosin staining was used to evaluate PDL fiber orientation and immunohistochemistry staining with semi-quantitative H-score analysis of MMP-8 and MMP-9 was performed. Results: At 24 h, MPO activity was significantly higher in untreated-diabetics than normoglycemics. At 24 and 48 h, the MDA level in untreated-diabetic rats was significantly higher than in normoglycemics and insulin-treated animals. At 72 h and 1 week, PDL fibers were oriented significantly more irregularly in untreated-diabetics than in normoglycemics. At all time points,MMP-8 and MMP-9 expressions were significantly higher in both diabetic groups than in the normoglycemic group. After the second day, tooth movement was significantly greater in untreated-diabetics than in the insulin-treated and normoglycemic groups. Conclusions: Mechanical stress in untreated-diabetic rats produces more inflammatory response, oxidative stress, tooth movement, PDL disorganization, and MMP-8 and MMP-9 expressions than among normoglycemics. Insulin reverses these effects, favoring the reorganization of periodontal ligament. Clinical relevance: Our results suggest that the application of orthodontic force in diabetic patients would increase inflammation and delay periodontal restructuring. Insulin would partly reverse this situation although glycemic decompensation episodes may occur. For these reasons, the periods between fixed orthodontic appliance activations should be of sufficient duration to allow adequate tissue recovery.
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Evaluating the efficacy of cinnamaldehyde and Echinacea purpurea plant extract in broilers against Eimeria acervulina
(Elsevier, 2011-09-15) Orengo, Juan; Ruiz de Ybáñez Carnero, María del Rocío; Madrid, Josefa; del Río, Laura; Catalá-Gregori, Pablo; García, V.; Hernández, Fuensanta; Buendía Marín, Antonio Julián; Sanidad Animal
Coccidiostats could be phased out as feed additives before 1 January 2013 for public health and food safety reasons, and, as a replacement, bioactive compounds found in plants are currently being investigated since they are more likely to be found acceptable by consumers. The aim of our study was to evaluate the effect of cinnamaldehyde (CIN) and Echinacea purpurea plant extract (EP) as additives by analyzing the performance traits, oocyst excretion and intestinal lesions following experimental infection with Eimeria acervulina. A total of 72 Ross male broilers were raised from 1 to 35 d and randomly assigned to four dietary treatments: control, without additives (C); 150 mg kg−1 cinnamaldehyde (CIN); 1000 mg kg−1 E. purpurea plant extract (EP); 150 mg kg−1 cinnamaldehyde plus 1000 mg kg−1 E. purpurea plant extract (CIN + EP). At 25 d, 12 chickens per treatment were orally infected with E. acervulina. Coccidia infestation led to lower performance but with no significant differences between the infected groups. Oocyst output reached its peak from 6 to 9 d post-infection in all treatments. At duodenal level, gross lesion scores were lower for cinnamaldehyde diets (P < 0.05). A similar trend was observed in the microscopic lesion scores, with a nonsignificant reduction as a result of cinnamaldehyde addition (P > 0.05). Scoring methods for macro- and microscopic lesions showed a positive linear relationship (G = +0.70). Further studies are necessary to assess the possible anticoccidian action of the cinnamaldehyde and its value as an alternative or adjunct in therapeutic or prophylactic strategies.
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Natural killer (NK) cells play a critical role in the early innate immune response to Chlamydophila abortus infection in mice
(Elsevier, 2004-01) Río, L. del; Caro, M. R.; Gallego, M. C.; Sánchez, J.; Cuello, F.; Salinas, J.; Martínez Cáceres, Carlos Manuel; Navarro Cámara, José Antonio; Ortega Hernández, Nieves; Buendía Marín, Antonio Julián; Anatomía y Anatomía Patológica Comparadas
Chlamydophila abortus, the aetiological agent of ovine enzootic abortion, induces a strong inflammatory reaction that leads to the T helper cell (Th1) specific immune response necessary for the clearance of infection. Because the role of natural killer (NK) cells during the first stages of this response has received little attention, this study focused on determining the function of these cells in a mouse model of infection. The location of NK cells in the liver and spleen of infected mice was examined immunohistochemically with an anti-Ly49G monoclonal antibody. The number of NK cells increased during the infection both in spleen and liver. In subsequent experiments, an anti-asialo GM1 polyclonal antibody was injected to deplete the NK cells. NK-depleted mice showed a substantial increase in their susceptibility to C. abortus infection, with high mortality rates and an increased burden of bacteria in the liver. Histopathological studies showed that inflammatory foci, composed mainly of neutrophils, were greater in size and number in depleted mice, while numerous chlamydial inclusions were associated with the foci. Serum concentrations of IFN-gamma, a key cytokine in the control of C. abortus infection, were substantially reduced in the NK-depleted mice. To establish the relationship between NK cells and other components of the innate immune response, neutrophils were depleted with the RB6-8C5 antibody. These cells were shown to be crucial in the recruitment of NK cells to the inflammatory foci.
Open Access
Comunicaciones Orales.-Influencia de las hormonas sexuales en la cinética de infección por Chlamydia abortus en modelo murino
(2020-05-28) Murcia Belmonte, Antonio; Álvarez Sánchez, Daniel; Gómez-Lucía Duato, Esperanza; Caro Vergara, María Rosa; Salinas Lorente, Jesús; Buendía Marín, Antonio Julián; Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Sanidad Animal; Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Anatomía y Anatomía Patológica Comparada
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Influence of the Th2 immune response established by Nippostrongylus brasilienses infection on the protection offered by different vaccines against Chlamydophila abortus infection
(Springer, 2005-03) Caro, M.R.; Gallego, M.C.; Cuello, F.; Ruiz de Ybáñez Carnero, María del Rocío; Erb, K.J.; Salinas, J.; Martínez Cáceres, Carlos Manuel; Ortega Hernández, Nieves; Buendía Marín, Antonio Julián; Sanidad Animal
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Canine carcinosarcomas in the head
(SAGE Publications, 2005-11) Sánchez, J.; Villafranca, M.; Velarde, R.; Altamira, J.; Martínez Cáceres, Carlos Manuel; Navarro Cámara, José Antonio; Buendía Marín, Antonio Julián; Anatomía y Anatomía Patológica Comparadas
Four cases of neoplasms in the heads of old dogs were studied. All the dogs showed both carcinomatous and sarcomatous malignant components in an admixed growing pattern. Histologic analysis of the tumors showed that the carcinomatous cells resembled squamous cell carcinoma in all dogs except one, where an adenoid arrangement of the neoplastic cells was also observed. The sarcomatous component showed osteoid matrix produced by pleomorphic poorly differentiated cells, which is regarded as a typical feature of osteosarcomas. Immunohistochemical analysis demonstrated that the components were positive only for cytokeratin (carcinomatous component) or vimentin (sarcomatous component). This observation led us to classify the neoplasms as true carcinosarcomas, thus providing evidence of a new preferential location for this unusual tumor in dog.
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Evaluation of a commercial double-recognition ELISA for caprine tuberculosis diagnosis: sensitivity, specificity, and correlation to macroscopic and microscopic lesions
(Elsevier, 2022-10-04) Salinas Lorente, Jesús; Del Río, Laura; Buendía Marín, Antonio Julián; Navarro Cámara, José Antonio; Ortega Hernández, Nieves; Sánchez Campillo, Joaquín; Sanidad Animal
Ante-mortem diagnosis of caprine tuberculosis, as in other ruminant species, is a complex process. Therefore, it is sometimes necessary to apply a combination of methods that increases the sensitivity of diagnosis while maintaining adequate specificity. In the present study, we analysed the use of ELISA based on a double-recognition methodology for the diagnosis of goat tuberculosis. Serum samples from animals with tuberculosis, confirmed by histopathological studies and presence of Ziehl-Neelsen-positive bacteria in the lesions, were used to assess sensitivity. Sera from flocks without tuberculosis were used to assess diagnostic specificity. The relationship between ELISA results and the type of tuberculous lesions, both macroscopic and microscopic, was analysed. The sensitivity and specificity of ELISA test were 69.9% and 97.9%, respectively. Sensitivity was higher in animals with macroscopic lesions, indicating more advanced forms of the disease. Similarly, a higher sensitivity was found in animals with microscopic lesions associated with active tuberculosis, such as cavitary lesions.