Histology and histopathology Vol. 6, nº 4 (1991)
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- PublicationOpen Accesslmmunocytochemical study of the epithelial lining of naturally occurring cysts in the rat intermediate lobe(Murcia : F. Hernández, 1991) Carbajo, S.; Carbajo-Pérez, E.; Hernández, J.L.; Vázquez, R.; Carvajal, J.C.An immunocytochemical study of the epithelial lining of naturally occurring cysts in the rat intermediate lobe (IL) has been carried out. Paraffinembedded sections, in which cysts were identifiable, were treated either with anti-serotonin or anti-S-100 protein sera. S-100-positive cells were intermingled with glandular cells surrounding the cyst lumen. These S-100- positive cells sent slender cytoplasmic processes as if to cover the apical surface of neighbouring cells. Rarely were 5-HT-immunopositive cells seen in the cyst epithelial lining. Most cells of the marginal layer of the IL were found reactive either to an S-100 or a-5-HT serum. The presence of an epithelial lining positive to S-100 protein sera is in keeping with the notion that cysts in the IL might form as evaginations of the epithelial lining of the pituitary cleft. The lack of correspondence between 5-HT-positive cells in the marginal layer and the cyst lining is controversial. A peculiar spatial relationship of 5-HT cells with the vascular network of the IL is suggested.
- PublicationOpen AccessSex-related differences in the nuclear population of postpubertal rat pineal gland. A quantitative study(Murcia : F. Hernández, 1991) López Muñoz, F.; Boya, J.; Calvo, J.L.Male and female parenchymal pineal cell types have been studied throughout postpubertal development to determine the existence of sex-related differences on a time basis. Six age groups (2, 3,4, 8, 15 and 24 months) of eight rats (4 males and 4 females) were used in this study. Nuclei of both parenchymal pineal cell types were counted in 5 areas of 26.377 pm' per pineal gland on semithin sections. Nonparametric statistics of our results (Mann-Whitney U-test and Kruskal-Wallis H-test) demonstrated significant differences between male and female pinealocytes through the stages studied. In all age groups, the number of nuclei per unit area was larger in female rats. Pineoglial cells did not show significant sex-related differences.
- PublicationOpen AccessThe distribution of peptide-containing nerves in the synovia of the cat knee joint(Murcia : F. Hernández, 1991) Abramobici, A.; Daizade, I.; Yosipovitch, Z.; Gibson, S.J.; Polak, J.M.Synovial capsule in cats is extensively innervated by a network with axonal diameter ranging from 0.6-3 pm according to its position and neuropeptide content. Nerve markers such as Neuron Specific Enolase (NSE) and Neurofilament triplet protein (NF) could be observed only when the axonal fibre attained a critical diameter of over the 3 pm limit. The relatively thick fibres (1-3 pm) show positive immunoieactivity for Substance P (SP), 5-hydroxytryptamine (5-HT). and Vasoactive Intestinal Peptide (VIP), and seldom coreact with NSE and NF. whereas, the thinnest fibres (0.6-0.8 pm) characterized to contain either Methionine or Leucine Enkephalin (M-Enk, L-Enk) did not coreact positively with axonal markers. We found that different anesthetics may effect variably the immunoreactivity of some neuropeptides (SP. L-Enk, 5-HT) while others (VIP, M-Enk) remained unaffected. Based on our data and the few reported ones in the pertinent literature, it is judged that urethane is the anesthetic of choice in experimental studies of neuropeptides. Our findings of isolated positive immunoreactive cell bodies to enkephalin in synovia might suggest the presence of intrinsic relay system, where the enkephalin acts as suppressor of SP and VIP release from the sunovium nerve terminals. Such a local inter-relationship between different neuropeptide systems might have a practical role on the understanding of the pathogenesis of different arthritic processes as well as therapeutic strategy in the future.
- PublicationOpen AccessChronic gastritis associated with Helicobacter pylori. Correlation between histological and bacteriological findings(Murcia : F. Hernández, 1991) Paz-Bouza, J.I.; García, I.; Abad, M.M.; Muñoz, Enrique Guillermo; Rincón, M.R.; Bullón, A.Biopsy specimens of gastric and duodenal mucosa from 326 patients were examined bacteriologically and histologically to determine the correlation between chronic gastritis and H. pylori colonization. H. pylori was identified in 111 (66.5%) patients with evidence of chronic gastritis and in 97 (82.2%) individuals who had gastritis associated with other pathology (gastric o duodenal ulcer, carcinoma o bulboduodenitis). The spiral bacteria was found more frequently in specimens with chronic superficial gastritis (881107) and no significant difference was observed between the grade of activity of gastritis and H. pylori colonization. Giemsa stain was the most suitable method for detecting H. pylori in histological sections. By electron microscopy the microorganism was seen on the surface of the gastric mucosa, beneath the mucous layer, and more occasionally in intercellular junctions and the gastric pit.
- PublicationOpen AccessUltrastructural study of the neuroglial and macrophagic reaction in Wallerian degeneration of the adult rat optic nerve(Murcia : F. Hernández, 1991) Carbonell, A.L.; Boya, J.; Calvo, J.L.; Marín, J.F.The Wallerian degeneration of the optic nerve of adult rat has been studied after destroying the retina. Animals were sacrificed between 4 days and 1 year after the lesion. Different cell types of the optic nerve have been studied ultrastructurally. Our results demonstrate the existence of a population of macrophages, probably of microglial origin, responsible for scavenging degenerated myelin. Astrocytes suffer a process of proliferation and hypertrophy. and are massively stuffed by gliofilaments, leading to a glial scar. These cells apparently do not participate in phagocytic phenomena, while some cytoplasmic inclusions (e.g. lipid droplets) suggest some implication in the local metabolization of some tissue degradation products. Oligodendrocytes do not undergo ultrastructural changes, showing a rather quiescent appearance.