Publication: Esterase activity (EA), total oxidant status (TOS) and total antioxidant capacity (TAC) in gills of Mytilus galloprovincialis exposed to pollutants: analytical validation and effects evaluation by single and mixed heavy
metal exposure
Authors
Franco, Lorena ; García-Navarro, José A. ; Teles, Mariana ; Tvarijonaviciute, Asta ; Romero García, Diego
item.page.secondaryauthor
item.page.director
Publisher
Elsevier
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1016/j.marpolbul.2015.12.010
item.page.type
info:eu-repo/semantics/article
Description
©2015 Elsevier. This document is the Published version of a Published Work that appeared in final form in Marine Pollution Bulletin. To access the final edited and published work see https://doi.org/10.1016/j.marpolbul.2015.12.010
Abstract
The aims of the present study were to optimize and validate methods for esterase activity (EA), total oxidant
status (TOS) and total antioxidant capacity (TAC) determination in mussel' gills, and to establish the relationships
between these biomarkers and Pb, Cd and Cu pollution, in single form and ternary mixture. Two different buffers
for sample homogenization, the need of ultracentrifugation, and analytical validation were evaluated. Coefficients of variation, when buffer without additives and ultracentrifugation were used, were b15%, and recovery
were 97%–109% in all cases. The EA response tends to decrease with treatments, TOS decreased significantly in
Cd and ternary groups, while TAC tended to increase in treatments with Pb, Cd and ternary groups. In conclusion,
the methods for EA, TOS and TAC measurements in gills of mussel were precise and accurate and could be interesting resources in biomonitoring programmes.
publication.page.subject
Citation
Marine Pollution Bulletin, Vol. 102, N. 1, 2016, pp. 30-35
item.page.embargo
Collections
Ir a Estadísticas
Sin licencia Creative Commons.