Publication: Canine C-reactive protein measurements in cerebrospinal fluid by a time-resolved immunofluorimetric assay
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Date
2011-01-01
Authors
Martínez Subiela, Silvia ; Caldin, Marco ; Parra Muñoz, María Dolores ; Ottolini, Nicola ; Bertolini, Giovanna ; Bernal, Luis J. ; García Martínez, Juan Diego ; Cerón Madrigal, José Joaquín
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Publisher
SAGE Publications
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DOI
https://doi.org/10.1177/104063871102300109
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info:eu-repo/semantics/article
Description
© 2011, American Association of Veterinary Laboratory Diagnosticians. This document is the Published version of a Published Work that appeared in final form in Journal of Veterinary Diagnostic Investigation. To access the final edited and published work see https://doi.org/10.1177/104063871102300109
Abstract
In the current study, the quantification of C-reactive protein (CRP) in cerebrospinal fluid (CSF) of dogs using an adapted time-resolved immunofluorimetric assay (TR-IFMA) was investigated, as well as whether the assay could be used to detect the range of CRP concentrations found in different clinical situations. Intra- and interassay coefficients of variation were below 15% in all cases. The TR-IFMA measured the CRP values in a proportional and linear manner (r 5 0.99); also CRP concentrations measured in CSF and in serum were significantly correlated (r 5 0.80, P 5 0.003). The limit of detection of the method was 7.1 3 1026 mg/l. The assay was able to detect differences in CRP concentrations in CSF of dogs with inflammatory disorders compared with dogs with spinal cord compression or idiopathic epilepsy. In conclusion, TR-IFMA constitutes a very sensitive, precise, and accurate method for the measurement of CRP concentrations in CSF.
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Citation
J Vet Diagn Invest 23:63–67 (2011)
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