Publication: Complex polysaccharide-based nanocomposites for 2 oral insulin delivery
Authors
Collado-González, Mar ; Freitas, Alessandra R. ; Santos, Ana Claudia ; Ferreira, Nuno F. ; Ferreri, Maria Cristina ; Sequeira, Joana A.D. ; Díaz Baños, F. Guillermo ; Víllora, Gloria ; Veiga, Francisco ; Ribeiro, Antonio ; Carissimi, Guzmán
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MDPI (Basel, Switzerland)
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Description
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
This document is the published version of a published work that appeared in final form in marine drugs
Abstract
Polyelectrolyte nanocomposites rarely reach a stable state and aggregation often occurs. Here, we report the synthesis of nanocomposites for the oral delivery of insulin composed of alginate, dextran sulfate, poly-(ethyleneglycol) 4000, Poloxamer 188®, chitosan, and BSA. Chitosan seems to be essential for the final size of the nanocomposites. When the chitosan concentration used in the synthesis is 10-4 gcm-3, nanocomposites, microcomposites and milimetric composites are obtained. Reducing the chitosan concentration to 10-5 or 10-7 gcm-3 yields nanocomposites of 400-600 nm or 800-900 nm hydrodynamic diameter, respectively, measured by DLS. According to our results, the molecular weight of chitosan has little influence on the size of the composites. The enhanced stability of the newest nanocomposites synthesized was assessed with LUMiSizer®. The rounded shapes of the nanocomposites were confirmed by scanning electron microscopy. Analysis by HPLC revealed that, after loaded with insulin, about 80% of the drug was released from our nanocomposites.
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