Histology and histopathology Vol.32, nº2 (2017)
Ir a Estadísticas
Permanent URI for this collection
Browse
Recent Submissions
Now showing 1 - 5 of 10
- PublicationOpen AccessAnti-inflammatory effects of enemas containing an oily extract of curcumin in an experimental model of diversion colitis(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Jaoudat Kadri, Caled; Aires Pereira, José; Guilherme Campos, Fábio; Marques Ortega, Manoela; Benediti Bragion, Celene; Real Martinez, Carlos AugustoCurcumin has powerful anti-inflammatory and antioxidant effects and it has been used for treatment of distal ulcerative colitis. The therapeutic effects of curcumin have not yet been evaluated in diversion colitis. The aim of the present study was to evaluate the anti-inflammatory effects of curcumin on colonic mucosa devoid of a faecal stream. Thirty-six rats were subjected to a proximal colostomy and distal colonic fistulation. They were divided into two groups, which were sacrificed two or four weeks after the intervention. Each group was divided into three subgroups treated with the daily application of enemas containing saline or an oily extract of curcumin at 50 mg/kg/day or 200 mg/kg/day. Colitis was diagnosed by histological analysis. Inflammatory grades were assessed using a previously validated scoring system. The infiltration of neutrophils was evaluated based on the tissue expression of myeloperoxidase (MPO), as determined by immunohistochemistry, and a computer-assisted image analysis program. The Mann-Whitney test was used to compare inflammation grades and myeloperoxidase levels among groups, and ANOVA was used to verify the variance over time, with the level of significance set at 5% (p<0.05) for both tests. Enemas containing curcumin improved the inflammation of the mucosa without a faecal stream and reduced the tissue contents of MPO. MPO tissue levels did not vary with time or between the concentrations of curcumin used. Enemas with curcumin improved the inflammation of the colonic mucosa, reduced the inflammatory grade and decreased the tissue content of MPO in colon segments without a faecal stream.
- PublicationOpen AccessStandardized grossing protocol is useful for the pathology reporting of malignant neoplasms other than adenocarcinomas(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Liszka, Łukasz; Mrowiec, Sławomir; Kuśnierz, Katarzyna; Kajor, MaciejBackground: There is no universally accepted protocol for gross examination of pancreaticoduodenectomy specimens. Standardized protocol (SP), known as Leeds Pathology Protocol, was previously validated in pancreatic adenocarcinoma. In this study we aimed to assess usefulness of SP in a series of specimens with pancreatic, ampullary, and duodenal malignant neoplasms other than adenocarcinomas. Materials and methods: SP was based on multi-colour inking and serial slicing of the specimens in a plane perpendicular to the duodenal axis. SP was used in a prospective cohort of 35 neoplasms of neuroendocrine, acinar, and solidpseudopapillary lineage (SP cohort). Surgical margin status, primary tumour stage, and lymph node yield in SP group were compared with corresponding data of a historical cohort of 19 cases examined using nonstandardized protocol (NSP). Samples examined in NSP and SP cohorts were comparable in terms of basic clinical characteristics, median tumour diameter, and distribution of histopathological diagnostic categories. Results: In SP cohort we noticed: (1) higher rate of detection of tumour tissue at surgical margins, (2) more frequent peripancreatic fat tissue invasion, (3) higher percentage of perineural invasion, (4) larger number of lymph nodes retrieved from the specimen, in comparison to NSP group. Application of SP was associated with significantly higher number of tissue blocks taken for histology. Conclusions: SP can be successfully applied for macroscopical examination of pancreaticoduodenectomy specimens with malignant pancreatic, ampullary, and duodenal neoplasms other than adenocarcinomas. SP with proper microscopical diagnosis enables an appropriate schedule of patients with these neoplasms to adjuvant therapy and surveillance programmes.
- PublicationOpen AccessEffect of coenzyme A on outer hair cells in cisplatin ototoxicity: functional and ultrastructural study(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Fernández Cervilla, Francisco; Martínez Martínez, María; Fernández Segura, Eduardo; Cañizares García, Francisco Javier; Crespo Ferrer, Pascual VicenteThe aim of this study was to use functional and morphological analyses to evaluate the protective effect of coenzyme A (CoA) in cisplatin-induced toxicity in outer hair cells (OHC). Three groups of 8 guinea pigs were used: control (group I), cisplatin-treated (group II) and cisplatin + CoA-treated (group III). In groups II and III, a single ototoxic dose of cisplatin (10 mg/kg) was injected intraperitoneally. Group III was co-treated with CoA (900 μg/kg per day for 7 consecutive days). Electrocochleography (ECoG) recordings were made before and after the 7-day treatment period in all groups. After ECoG on day 7, all animals were anesthetized and the cochleae were removed and fixed for ultrastructural analysis. Cell damage in OHC was observed with transmission electron microscopy. Cisplatin induced a significant increase in auditory thresholds (p<0.001) compared to group I (control). In contrast, group III (cisplatin + CoA) had significantly reduced thresholds (p<0.001) compared to the group treated with cisplatin alone (group II). We found no significant differences between the control group and animals co-treated with cisplatin and CoA. The electron microscopy findings in OHC were consistent with these results. Ultrastructural analysis of OHC in group II showed morphological indications of necrosis, i.e. cytoplasmic swelling and vacuolation, and mitochondrial swelling. In group III the cell morphology of OHC was preserved, with ultrastructural characteristics similar to the control group. In conclusion, co-treatment with cisplatin with CoA inhibited antineoplastic-induced cytotoxicity in OHC in a guinea pig model.
- PublicationOpen AccessImmunohistochemical analysis of thymidylate synthase expression in gastric carcinoma: correlation with clinicopathological parameters and survival(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Rogoza Mateja, Wiesława; Domagala, Pawel; Kaczmarczyk, Mariusz; Mieżyńska Kurtycz, Joanna; Ławniczak, Małgorzata; Sulżyc Bielicka, Violetta; Bielicki, Dariusz; Karpińska Kaczmarczyk, Katarzyna; Domagala, WenancjuszThe correlation of thymidylate synthase (TS) expression in gastric cancers with tumor histology and prognostic or predictive information remains unclear. Most studies have involved Asian populations, with few conducted in European cohorts. Moreover, all published studies analyze TS expression using semi-quantitative methods. This retrospective study evaluated the association of TS expression in tumor cells with gastric carcinoma histological type, with selected clinicopathological parameters, and with the prognosis of patients who underwent surgical treatment. TS expression was detected using immunochemistry and objectively assessed by computerized image analysis of tumor cells in 100 gastric cancers. We found that high TS expression was significantly more common in intestinal than in diffuse type of gastric cancer according to Lauren classification (P=0.0003); in type I carcinomas compared to type IV according to Goseki classification (P=0.002); and in gastric cancers in men than women (P=0.04). Low TS expression was found more often in carcinomas in the middle and lower third of the stomach than in cancers in the upper third of the stomach (P=0.009 and P=0.001, respectively). In the subgroup of 25 patients without lymph node metastases (stage I+II), high TS expression was associated with better DFS (83% for high TS expression versus 38,5% for low TS expression, P=0.03). The results: (1) indicate significant correlation between the Lauren and Goseki histopathological classifications of gastric cancer and TS expression in tumor cells, (2) suggest that high TS expression may be a positive prognostic marker with regard to DFS in patients with gastric cancer without involvement of regional lymph nodes who underwent radical surgical treatment and were not treated with preoperative chemotherapy. Prognostic results need confirmation in larger cohorts.
PublicationMetadata onlyAn immunohistochemical study of NFE2L2, KEAP1 and 8-hydroxy-2'-deoxyguanosine and the EMT markers SNAI2, ZEB1 and TWIST1 in metastatic melanoma(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Hintsala, Hanna Riikka; Haapasaari, Kirsi Maria; Soini, Ylermi; Karihtala, PeeterBackground: Little is known regarding the role of redox balance regulators in metastatic melanomas, but there is some evidence for a link between epithelial-to-mesenchymal transition (EMT) and cellular redox status. Methods: We compared the immunohistochemical expression of nuclear factor erythroid-2-related factor 2 (NFE2L2), Kelch-like ECH-associated protein 1 (KEAP1), 8-hydroxy-2'-deoxyguanosine (8-OHdG), TWIST1, SNAI2 and ZEB1 between primary melanomas and metastases in a cohort of 23 nevi, 66 malignant melanomas and 22 metastases. Results: Nuclear NFE2L2 expression was higher (p=0.003) and cytoplasmic KEAP1 lower (p=0.026) in metastatic lesions than at primary sites. Nuclear NFE2L2 expression was associated with the presence of distant metastases (p=0.040) and with nuclear TWIST1 expression (p=0.002). Patients having both NFE2L2 and TWIST1 expression in nuclei had an extremely poor prognosis (p=0.0003). In multivariate analysis nuclear TWIST1 expression was an independent predictor of a poorer prognosis (HR 2.99, 95% CI 1.17-7.69; p=0.023) and the invasive TWIST1/ZEB1 phenotype showed poorer melanoma-specific survival (HR 7.28, 95% CI 2.23-23.77; p=0.001). Nuclear expression of 8-OHdG (p=0.001) was lower at metastatic sites than in primary lesions. Conclusions: EMT signalling and the KEAP1/NFE2L2- axis are likely to be involved in metastatic spread of malignant melanoma and also appear to have potential interactions.