Publication: Anti-inflammatory effects of enemas containing an oily extract of curcumin in an experimental model of diversion colitis
Authors
Jaoudat Kadri, Caled ; Aires Pereira, José ; Guilherme Campos, Fábio ; Marques Ortega, Manoela ; Benediti Bragion, Celene ; Real Martinez, Carlos Augusto
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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Description
Abstract
Curcumin has powerful anti-inflammatory
and antioxidant effects and it has been used for treatment
of distal ulcerative colitis. The therapeutic effects of
curcumin have not yet been evaluated in diversion
colitis. The aim of the present study was to evaluate the
anti-inflammatory effects of curcumin on colonic
mucosa devoid of a faecal stream. Thirty-six rats were
subjected to a proximal colostomy and distal colonic
fistulation. They were divided into two groups, which
were sacrificed two or four weeks after the intervention.
Each group was divided into three subgroups treated
with the daily application of enemas containing saline or
an oily extract of curcumin at 50 mg/kg/day or 200
mg/kg/day. Colitis was diagnosed by histological
analysis. Inflammatory grades were assessed using a
previously validated scoring system. The infiltration of
neutrophils was evaluated based on the tissue expression
of myeloperoxidase (MPO), as determined by
immunohistochemistry, and a computer-assisted image
analysis program. The Mann-Whitney test was used to
compare inflammation grades and myeloperoxidase
levels among groups, and ANOVA was used to verify
the variance over time, with the level of significance set
at 5% (p<0.05) for both tests. Enemas containing
curcumin improved the inflammation of the mucosa
without a faecal stream and reduced the tissue contents
of MPO. MPO tissue levels did not vary with time or
between the concentrations of curcumin used. Enemas
with curcumin improved the inflammation of the colonic
mucosa, reduced the inflammatory grade and decreased
the tissue content of MPO in colon segments without a
faecal stream.
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