Histology and histopathology Vol.10, nº 1 (1995)
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- PublicationOpen AccessHistochemical and ultrastructural features(Murcia : F. Hernández, 1995) Fekete, Eva; Resch, B.A.; Benedeczky, I.The developing enteric nervous systern of the hurnan foetus has been analyzed at the 10th and 18th week of gestation with a special reference to the development of nerve-rnuscle contacts. The rnyenteric plexus forrnation was analyzed by rneans of electron microscopy and on whole-mounts after NADH diaphorase histochernistry. The developrnent of noradrenergic innervation as an extrinsic inhibitory supply was followed by means of a glyoxylic acidinduced fluorescence rnethod. Differentiated neurons and neuroblasts both occurred in rnyenteric ganglia of the 10- and 18-week-old foetus although the ganglionic neuropil was almost unidentifiable ultrastructurally at the 10th week of gestation but was rnature looking at the 18th week. The nerve plexuses connecting the ganglia frequently forrned distant and close rnyoneural contacts. NADH-diaphorase histochernistry revealed that in the 18-week-old hurnan foetus rnost of the neural perikarya was within the ganglia. Also, at the 18th week of gestation a well-defined fluorescent network was dernonstrated within the ganglia, as well as in the internodal segrnents. On the basis of these observations we concluded that the time between the 10th and 18th week of gestation has pararnount irnportance for both the rnorphological and functional rnaturation of the enteric nervous systern.
- PublicationOpen AccessStructure and function of melanocytes, Microscopic(Murcia : F. Hernández, 1995) Hirobe, T.Melanocytes characterized by their tyrosinase activity, melanosomes and dendrites locate in the basa1 layer of epidermis and hair bulb in the skin of mice. Melanocytes differentiate from undifferentiated melanoblasts derived from embryonic neural crest. Melanocyte-stimulating hormone plays an important role in the regulation of the differentiation of mouse melanocytes in the epidermis and hair bulb by inducing tyrosinase activity, melanosome formation, transfer of melanosomes and increased dendritogenesis. The proliferative activity of differentiating epidermal melanocytes of newborn mice during the healing of skin wounds is regulated by semidominant genes, suggesting that the genes are involved in regulating the proliferative activity of epidermal melanocytes during differentiation. The morphology and differentiated functions of mouse melanocytes are shown to be influenced by environmental factors such as ultraviolet and ionizing radiations. From the results of serum-free culture of mouse epidermal melanoblasts, basic fibroblast growth factor is shown to stimulate the sustained proliferation of melanoblasts in the presence of dibutyryl adenosine 3 ' 3 - cyclic monophosphate and keratinocytes. In contrast, melanocyte differentiation in serum-free culture is induced by melanocyte-stimulating hormone in the presence of keratinocytes. These results suggest that the structure and function of mouse melanocytes in the epidermis and hair bulb are controlled by both genetic factors and local tissue environment, such as hormones and growth factors.
- PublicationOpen AccessThe use of microwave irradiation for immunohistochemistry(Murcia : F. Hernández, 1995) Vitarelli, E.; Sippelli, G.; Tuccari, G.; Barresi, G.We developed a rapid immunohistochernical method using a microwave oven in formalin-fixed, paraffin-embedded sections from normal and pathological tissues. The strongest immunoreactivity was obtained for actin, Ca 125, CEA, pan-cytokeratin, chromogranin A, EMA, GFAP, thyroglobulin, kappa and lambda chains. In control tissues, processed with conventional immunocytochemical procedure, the reactivity was found to be qualitatively and quantitatively similar. Dako EPOS kits were also assayed with good staining intensity, shortening the original technique to 16 min. Our microwave immunohistochemical method is simple, rapid and it may be recommended for use in routine laboratories.
- PublicationOpen AccessLectin-binding sites in the epithelium of normal(Murcia : F. Hernández, 1995) Brinck, U.; Bosbach, R.; Korabiowska, M.; Schauer, A.; Gabius, H.J.By using histochemical methods, the binding pattern of various lectins in the epithelium of normal human appendix vermiformis was assessed. In addition to plant and invertebrate sugar receptors with nominal monosaccharide specificity for a-L-Fuc (UEA-1), a-DMan and a-D-Gluc (Con A), a-D-GalNAc (DBA), DGalNAc (SBA, HPA) B-D-Gal (RCA-I) and D-Gal (VAA), a mammalian B-galactoside-specific lectin (MW, 14 kDa) was included in the applied panel. The apical surface of enterocytes presented binding sites for RCA-1 on al1 cells, binding sites of UEA-1, DBA, SBA, HPA and VAA heterogeneously and no binding sites of Con A and 14 kDa. Binding sites of DBA, SBA, HPA, VAA and RCA-1 within enterocytes were located primarily focally in a supranuclear position, whereas Con A and 14 kDa bound to the cytoplasm both in apical and basa1 cell parts. In the follicle-associated epithelium more enterocytes expressed SBA- and VAA-binding sites than in the crypt epithelium. No differences between the lectin-binding pattern of M-cells and enterocytes were found in the follicle-associated epithelium. Intraepithelial macrophages were heterogeneously positive for the full panel of applied lectins. In contrast, intraepithelial lymphatic cells expressed binding sites only for RCA-1 and less prominently for Con A, VAA and 14 kDa. Goblet cell mucus contained lectin-binding sites in a heterogeneous manner: binding sites for Con A were not detected in goblet cells for DBA, SBA, VAA and 14 kDa in less than 20%, for UEA-1 in 20-40%, for HPA in 40-60% and for RCA-1 in 60-100% of the goblet cells. Secreted mucus differed in its lectin-binding capacity from intracellular goblet cell mucus selectively by an increase of UEA-1, SBA- and RCA-1-binding sites and a lack of 14 kDa-binding sites. Comparative study of lectin binding to goblet cell mucin in another region of the large intestine, namely the rectosigmoid, demonstrated that DBA, SBA and 14 kDa bound mainly to the dista1 colon, while UEA-1 and VAA labelling was selectively found in Oífprint reguests lo: Dr. Ulrich Brinck, M.D., Department of Pathology, University of Gottingen, Robert-Koch-Str. 40, D-37075 Gottingen, Germany apendiceal goblet cell mucin. Comparing the lectin-binding pattern in normal appendix epithelium and in appendicitis, the percentage of goblet cells expressing DBA- and SBA-binding sites in mucus globules was found to be about 4 times higher in appendicitis than in normal appendix. These results demonstrate that the expression of lectin-binding sites in appendiceal goblet mucin is specifically altered in appendicitis, indicating that there are selective changes of glycosylation of mucin in goblet cells mainly of the lower and middle crypt segment. Changes of lectinbinding pattern in appendicitis are discussed in connection with histochernical findings in inflammatory bowel disease.
- PublicationOpen AccessGiant cell tumor of the ovary. lmmunohistochemical(Murcia : F. Hernández, 1995) Franco, V.; Florena, A.M.; Orlando, E.; Becchina, G.Giant cell tumor (GCT) of the ovary ic a rare condiiion, fouiid almost invariably in the context of a mucinous iumor and presenting a microscopic picture indistinguishable frorn GCT of bone. We describe a case of GCT in the wall of a serous cyst of the ovary. An iinmunohistocheniicr~l study was performed using a panel of antibodies to epithelial, niesenchymal and leukocyte antigens. Mononuclear arid giant tumor cells were positive for vimentin; CD 68 and LCA were found only in giant cells whereas actin was only found in inononuclear tumor cells. The immunophenotypic profile of the stronial cells of the residual o\!ary was identical to that of riiononuclear tumor cells. The pi-esentcd data suggest that GCT of the ovary is probnbly ri non-neoplastic lesion of the mesenchyinal stronial cells that react against substances of the associated tuinor or cyst.