Histology and histopathology Vol.10, nº 1 (1995)
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- PublicationOpen AccessEffects of bombesin on the morphology and(Murcia : F. Hernández, 1995) Malendowicz, L.K.; Nussdorfer, G.G.; Miskowiak, B.; Majchrzak, M.The acute and chronic effects of bornbesin (BM) on the structure and function of rat adrenal cortex were investigated by rnorphornetric and radioirnrnunological techniques. An intraperitoneal bolus injection of 2 yglrat BM rnarkedly raised plasma corticosterone (B) concentration (PBC). The intraperitoneal BM infusion (1 yg/rat.h-l) for 1, 2 or 4 days evoked a notable increase in the nurnber of adrenocortical cells, without inducing apparent changes in either PBC or B output by adrenal quarters. Since proliferation and expression of specialized functions are rnutually exclusive states of cells, our findings suggest that the conspicuous stirnulation of adrenocortical-cell proliferation evoked by BM infusion may be responsible for the apparent lack of effect of this treatrnent on B secretion.
- PublicationOpen AccessEpithelial lung cell marker, current tools for cell typing(Murcia : F. Hernández, 1994) Kasper, M.; Singh, G.This review discusses current irnmunohistochemical and lectin histochemical approaches to identify and to distinguish the different epithelial cell populations of the pulmonary tissue. Special emphasis is given to the characterization of pulmonary alveolar and bronchial epithelial cells and mesothelial cells. Structural proteins, membrane molecules and secretory products of the alveolar epithelium, which have already been characterized and which may be useful for monitoring the developmental or pathological processes, are listed and briefly described.
- PublicationOpen AccessGiant cell tumor of the ovary. lmmunohistochemical(Murcia : F. Hernández, 1995) Franco, V.; Florena, A.M.; Orlando, E.; Becchina, G.Giant cell tumor (GCT) of the ovary ic a rare condiiion, fouiid almost invariably in the context of a mucinous iumor and presenting a microscopic picture indistinguishable frorn GCT of bone. We describe a case of GCT in the wall of a serous cyst of the ovary. An iinmunohistocheniicr~l study was performed using a panel of antibodies to epithelial, niesenchymal and leukocyte antigens. Mononuclear arid giant tumor cells were positive for vimentin; CD 68 and LCA were found only in giant cells whereas actin was only found in inononuclear tumor cells. The immunophenotypic profile of the stronial cells of the residual o\!ary was identical to that of riiononuclear tumor cells. The pi-esentcd data suggest that GCT of the ovary is probnbly ri non-neoplastic lesion of the mesenchyinal stronial cells that react against substances of the associated tuinor or cyst.
- PublicationOpen AccessVessel wall-dependent metabolic pat hways of the(Murcia : F. Hernández, 1995) Preissner, K.T.; Pötzsch, B.The integrity of the vessel wall under quiescent conditions as well as its appropriate responsiveness under conditions of stimulation, inflammation or vascular injury is controlled by a number of adhesive interactions involving distinct cellular receptor systems and various multifunctional adhesive ligands. While a number of these extracellular matrix components of the vessel wall are endogenously produced, secreted and deposited, exogenous adhesion proteins may become translocated from the intra- to the extravascular space by virtue of endothelial cell-specific transport systems. Two prominent examples for each metabolic pathway are discussed. Endothelial cellspecific biosynthesis and secretion as well as deposition of multimeric von-Willebrand-factor within intracellular granules (Weibel-Palade bodies) relates to the first possibility of processing, whereas binding of reactive forms of circulating vitronectin to diverse cellular receptors with subsequent extravasation and deposition into the extracellular matrix appears to be characteristic for the second case. In this review the known features of the metabolic routes of both adhesion proteins are discussed and set in perspective to their functional properties. Their localized mode of action in the vessel wall appears to be crucial for balanced haemostasis and immune systems, two major defence mechanisms of the organism.
- PublicationOpen AccessEffects of various antineoplastic agents on-2(Murcia : F. Hernández, 1995) García Reverte, Juana María; Bernabeu-Esclapez, A.; Muñoz Ramos, J.; Vicente Ortega, Vicente; Canteras Jordana, ManuelAn established human melanoma cell line was treated with severa1 concentrations of three antineoplastic drugs: melphalan (0.01 6, 0.032, 0.16 pn), CCNU (1- (2-chloroethy1)-3-cyclohexyl1-- nitrosourea) (0.04, 0.21, 0.42 pM) and 4-OHA (4-hydroxyanisole) (4.01 x 1 0-4, 1 . 2 0~1 0 -2~.,4 ~ pM), and the effects on cell growth and viability were compared. 24 hours after treatment, 4-OHA ( 1 ~ ~ ~ = 2 . 4 ~ 1 0 - ~ pM) was more cytotoxic than melphalan (ID50=0.016 pM) and CCNU (ID50=0.21 pM). However. after 96 hours exposure, the most effective drug was CCNU (growth rate = -1.277), which caused the death of the culture. This was followed by melphalan (growth rate = - 1.024) and finally 4-OHA (growth rate= -0.69). Similar ultrastructural cell injuries were observed after the use of the three drugs: the dilation of endoplasmic reticulum vesicles and the nuclear membrane; mitochondria swelling; and the existence of lamellar structures and cytoplasmic vacuoles.