Histology and histopathology Vol. 1, nº 3 (1986)

Ir a Estadísticas

Permanent URI for this collection

http://hdl.handle.net/10201/177591

Browse

Recent Submissions

Now showing 1 - 5 of 13
  • Thumbnail Image
    Publication
    Open Access
    Substance P-like immunoreactivity in rat and cat carotid bodies, Light and electron microscopic studies
    (Murcia : F. Hernández, 1986) I-li Chen; Yates, Robert D.; Hansen, John T.
    Substance P-immunoreactive (SP-I) structures in the carotid bodies of rats and cats were examined with the light and electron microscopes. In both species SP-I varicose nerve fibers were located singly in the interstitial connective tissue in close association with blood vessels. They were small unmyelinated fibers enveloped in a common Schwann cell sheath with other SP-negative fibers. Some of SP-I fibers contained large dense-cored granules and small clear vesicles in addition to microtubules and mitochondria and probably represented nerve fiber varicosities. The latter often were found incompletely invested by Schwann cell sheaths. SP-fibers were found occasionally in the envelopes of supporting cells at the periphery of parenchymal cell groups. However, none of the nerve terminals making synaptic contacts with glomus cells exhibited SP-like immunoreactivity. In cat carotid bodies some glomus cells showed moderate to intense SPlike immunoreactivity.The intense SP-I glomus cells displayed numerous dense-cored vesicles of 85 to 140 nm in diameter and frequently showed synaptic contacts with SP-negative nerve terminals. In rat carotid bodies we were unable to detect consistent SP-immunoreactivity in glomus cells. Our results do not favor the hypothesis that SP is a neurotransmitter/modulator in the chemoreceptor afferents synapsing on glomus cells in either the cat or rat carotid body. However our results support the hypothesis that SP in cat glomus cells may play a role in the modulation of chemoreceptor activity.
  • Thumbnail Image
    Publication
    Open Access
    Observations on cell kinetics and viability of a human melanoma cell line exposed to dicarboxylic acids in tissue culture
    (Murcia : F. Hernández, 1986) Breathnach, A. S.; Robins, E.J.; Bhasin, Y. P.; Ethridge, L. B.; Nazzaro-Porro, M.; Passi, S.; Picardo, M.
    Cultures of human nlelanonla cell line BOO08 \verc e-iposed to the disodium salts of arelaic acid (C,, 2Na), adipic acid (C,, 2Na) and dodecanediaic acid (C,, 2Na) at IO'M and 5 x 10-'M for 24 hrs. None of the diacid salts had a significant effect on growth rate or viability of the cells, at 10-'M for 24 hrs nor had C, 2Na any effect at 5 x 10'M. At 5 x 102M for 24 hrs, both C,, 2Na, and CIL2 Na had a significant effect in reducing both growth and viability. These effects were accompanied by morphological evidence of cell death, and swelling of mitochondria and accuni~~latioonf lipid droplets within cytoplasm of still biable cells.
  • Thumbnail Image
    Publication
    Open Access
    The neurotoxic effect of monosodium glutamate (MSG) on the retinal ganglion cells of the albino rat
    (Murcia : F. Hernández, 1986) van Rijn, C.M.; Marani, E.; Rietveld, W.J.
    Monosodium glutamate (MSG) administered postnatally to the albino rat causes extensive destruction of the retina. This MSG effect does not result in complete blindness. Ganglion cells surviving the MSG treatment are healthy and functional. Using retrogradally transported HRP and Nissl itaining in whole mounted retinas, it \vas found that the ganglion cells left after MSG treatment are nut smaller than those in controls, that these cells do not belong to one cell size group, and that no cell size group is selectively missed. The results explain why photic entrainment of MSG treated animals is still possible.
  • Thumbnail Image
    Publication
    Open Access
    lmmunohistochemical reaction of myocardial fibers with actin antiserum in autopsy cases of myocardial infarction
    (Murcia : F. Hernández, 1986) Shigeo Hashimoto; Shozo Nishida; Shingo Hiruma; Manabu Takahashi; Yoshiki Enomoto; Kuniyasu Sakatani
    The purpose of this study was to determine the immunoreactivity of myocardial actin filaments with actin antiserum and to examine the significance of its application to diseased human cardiac muscle. The actin was extracted and purified from chicken gizzards. Anti-actin rabbit serum was prepared and purified by affinity chromatography and defined by an immunoblotting test. Using the avidin-biotin-peroxidase complex (ABC) method, the actin antiserum was applied to paraffin sections prepared from hearts taken from routine autopsies of patients who had died of myocardial infarction. Reactivity was shown to be completely lost, not only in necrotized fibers, but also in non-specific degenerative fibers which could be identified by their eosinophilic cytoplasm with pyknotic nuclei, and clearly remaining and/or diminished cross-striations stained with hematoxylin-eosin. In contrast, hypertrophic myocardial fibers adjacent to granulation or scar tissue and those adjacent to infarcted foci exhibited a more intense reaction. These results indicated that the immunohistochemical reaction of actin filaments can be used for the easy detection of very mild degrees of degeneration of cardial muscle fibers, and for hypertrophic fibers adjacent to diseased foci. Studies of the immunoreactivity of actin protein suggestive of alteration at the molecular level might yield morphological clues regarding the nature of functional activity in the contraction of cardiac fibers.
  • Thumbnail Image
    Publication
    Open Access
    Effects of mellitic acid (MA) and sodium fluoride (NaF) on the histological appearance of murine fetal tibiae cultured in vitro
    (Murcia : F. Hernández, 1986) Weischer, C.H.; Krisinger, J.; Karzel, K.
    The aim of this studv was to develop a standardized image analysis method for localization and quantitative measurement of calcified structures of murine fetal tibiae cultured in vitro as a completion and verification of previous biochemical studies. The calcified structures of bone stained by von Kossa silver technique and the epiphyseal cartilages showing intensive metachromasia with toluidine-blue staining were converted with grey-value window programs and afterwards the areas of the selected structures were measured. The histomorphological investigations showed that the murine tibiae, incubated for a period of 6 days in a medium with addition of 5 mmol mellitic acid, showed both a significant reduction of calcium deposits and an increase of epiphyseal intercellular cartilage matrix. The tibiae incubated in a medium with addition of 0.5 mmol sodium fluoride significantly showed an increase of calcium deposits in the thickened lammellae of the cornpacta. These histomorphological results confirm previous biochemical studies.