Histology and histopathology Vol. 2, nº 2 (1987)

Ir a Estadísticas

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https://hdl.handle.net/10201/17684

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Light and electron microscopic study of fetal lung following maternal exposure to methylmercuric chloride
(Murcia : F. Hernández, 1987) Curle, D.C.; Ray, M.; Persaud, T.V.N.
Varying dose levels of methylmercuric chloride (MMC), 1000 ppm (5 mg through 15 mglkg of body weight), were administered via an intragastric tube to pregnant ICR SwissIWebster mice on day 9 of gestation. The animals were killed on gestational day 18 and the fetuses removed. Fetal lung sections were processed for light and electron microscopy. A group of animals treated with physiological saline in a similar mannner served as the controls. The fetal lungs from treated animals were hypoplastic and retarded in development. The severity of pulmonary changes increased with the dose-levels of MMC. Vacuolation and lysis of mitochondria were seen in fetal lungs. Mitochondrial damage increased in severity with doselevel of methylmercuric chloride.
Open Access
Immunocytochemical studies on prolactin cells in the adenohypophysis of the golden hamster
(Murcia : F. Hernández, 1987) Seu-Mei Wang; Chia-Man Lue; Huai-San Lin
Mammotrophs or prolactin (PRL) cells were identifed in the adenohypophysis of adult golden hamsters by immunocytochemical techniques with a polyclonal anti-PRL, that was proved to be specific to PRL by the dot immunoblotting test. Postembedding immunostaining was performed on Araldite thin sections by immunoperoxidase and immunogold methods. PRL cells were classified into three types according to the different size of the secretory granules. The Type A cells were usually small and angular or oval in shape, and had secretory granules ranging in diameter from 100-230 nm, and showed poorly developed organelles. The Type B and C cells were larger and round or ovoid in shape, contained larger granules, 230-280 nm and 280-570 nm, respectively, and displayed well developed organelles. Immunoreactive PRL cells in the male pituitaries were far less numerous than in the nonpregnant female glands, and were mostly of the Type A and B, whereas in the female the Type C and B cells predominated. In pregnant females, Type C cells became activated and increased in number, while the other two types decreased in proportion. In lactating females, Type A and B cells significantly increased in number at the expense of the Type C cells; meanwhile, the exocytosis of secretory granules was frequently found in all types of PRL cells. The present findings suggest that Type C and B PRL cells, especially the former, are potent in producing and releasing PRL and highly responsive to various physiological stimuli, while Type A cells are probably relatively inert in synthetic activity.
Open Access
The protective effects of a prostaglandin without antisecretory properties aginst ethanol-induced injury in the rat stomach, a histologic study
(Murcia : F. Hernández, 1987) Schmidt, Carmen L.; Henagan, Julia M.; Mitchell, Philip A.; Smith, Gregory S.; Miller, Thomas A.
This study examined the effect of 2-acetyl-2- decarboxy-15(S)-15 methyl PGF,, (PGF,,) on ethanol (EtOH) induced injury in the rat stomach to determine if a PG analogue devoid of antisecretory properties could confer full or partial gastric mucosal protection. Rats were orally administered saline or PGF,, in a dose of 0.5 or 5.0 mg1Kg. Thirty minutes later animals received varying concentrations (i.e. 25%, 50%, and 100%) of EtOH orally. Five minutes following EtOH exposure, they were killed and samples taken from identical regions of the glandular mucosa for microscopic evaluation. All concentrations of EtOH tested damaged the gastric epithelium. The injury induced by 25% EtOH was almost exclusively confined to the surface epithelium and was not altered by either dose of PGF,,, pretreatment. In contrast, both 50% and 1(10% EtOH elicited comparable damage to the gastric mucosa involving both the deep and superficial mucosa of virtually the entire epithelium. The deep injury induced by these two EtOH concentrations was prevented by both the low and high dose of PGF,,. Of particular importance the 5.0 mg dose of PGF, provided complete protection (i.e. both superficial and deep) to as much as 50% of the mucosa exposed to 50% or 100% ethanol. These findings indicate that PGF?, possesses ~~cytoprotectivep*r operties involving both the superficial and deep epithelium that are dose related.
Open Access
Plasma cell granuloma of the tongue. Report of a case
(Murcia : F. Hernández, 1987) Soares, J.; Moura Nunes, J.F.; Sacadura, J.
A case of plasma cell granuloma of the tongue in an otherwise symptomless 48-year-old caucasian female is reported. The polyclonal nature of the plasmocytes was revealed by immunostaining of kappa and lambda light chains. Electron microscopic observations showed typical mature plasmocytes. A parasitic etiology of this type of lesion is suggested.
Open Access
Endocrine cells of the gastric mucosa of Rana temporaria L.
(Murcia : F. Hernández, 1987) Díaz de Rada, O.; Sesma, P.; Vazquez, J.J.
The endocrine cells of the gastric mucosa of Rana temporaria have been studied according to the ultrastructure, the staining properties of the granules with Masson Fontana's and Grimelius' silver methods, silver impregnation of Davenport on deplasticised semithin sections and immunocytochemical techniques. Seven different types of endocrine cells have been described. Six were regarded as belonging to known types: G, A, EC, ECL, D and P cells. One type was considered as unclassifiable.

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