Histology and histopathology Vol.34, nº2 (2019)
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- PublicationOpen AccessExpression of glutamine metabolism-related proteins in Hürthle cell neoplasm of thyroid: Comparison with follicular neoplasm(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Cha, Yoon Jin; Jang, Haerin; Koo, Ja SeungPurpose. We evaluated the expression of glutaminolysis-related proteins in Hurthle cell neoplasms (HCN) and follicular neoplasms (FN) of the thyroid, and investigated its clinical implication. Methods. Tissue microarrays were constructed from 264 FNs (112 follicular carcinomas [FCs] and 152 follicular adenomas [FAs]) and 108 HCNs (27 Hurthle cell carcinomas [HCCs] and 81 Hurthle cell adenomas [HCAs]. The immunohistochemical staining result of 3 glutaminolysis-related proteins (Glutaminase 1 [GLS1], glutaminate dehydrogenase [GDH] and alanine- serine, cysteine-preferring transporter 2 [ASCT2]) was analyzed. Results. GLS1 and GDH showed significantly higher expression rates in HCN compared to FN (P<0.001). More HCN cases showed co-positivity of multiple glutaminolysis-related proteins than those of FN cases (P<0.001). In silico analysis, both GLUD1 and GLUD2 showed higher expression rate in HCA compared to FA (P=0.027 and P=0.018, respectively). SLC1A5 expression was highest in HCA, followed by FC and FA (HCA vs FC, P=0.023; FC vs FA, P=0.002). Conclusion. FN and HCN exhibit a different expression pattern for glutaminolysis-related proteins, and GLS1 and GDH have higher expression rates in HCN and FN.
- PublicationOpen AccessMature congenital intraneural teratoma in cerebellum of pig(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Soto Domíngue, Adolfo; Rodríguez Rocha, Humberto; García García, Aracely; Saucedo Cárdenas, Odila; Rodríguez Tovar, Luis E.; Castillo Velázquez, UzielThe biological behavior of teratomas depends on several interdependent clinical and epidemiological variables such as age at diagnosis, sex, tumor microenvironment, and tumor morphology, among others. All these variables are correlated to different cytogenetic and molecular aberrations (Harms et al., 2006). There are null reports of teratomas in pigs. The aim of this study was to characterize the tissues present in a mature congenital intraneural teratoma in the cerebellum area of a Landrace female pig of 6-7 weeks old. In this study, tissue control samples were used to validate each staining method. Sections from the teratoma showed normal histology of the cerebellum, including rounded Purkinje neurons with abundant cytoplasm, euchromatic nuclei, and prominent nucleoli; glial cells with a scarce amount of cytoplasm and small and highly basophile-nuclei (compact chromatin) and axonal tracts (white matter). Interestingly, we also observed areas with tissues different from the nervous tissue, including bundles of well-defined skeletal muscle fibers with a striated pattern and peripheral nuclei; hyaline cartilage plaques, with prominent presence of chondrocytes in their lagoons forming isogenous groups surrounded by a territorial and interterritorial matrix; trabeculated bone tissue; and adipocytes, which are ringshaped cells with peripheral flattened nuclei, as a result of the presence of a central large lipid droplet. To our knowledge, this study is the first to describe a congenital intraneural mature teratoma in the cerebellum of a pig.
- PublicationOpen AccessLipopolysaccharide induces the early enhancement of mice colonic mucosal paracellular permeability mainly mediated by mast cells(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Sun, Tingyi; Wang, Yaxi; Hu, Shilong; Sun, Haimei; Yang, Shu; Wu, Bo; Ji, Fengqing; Zhou, DeshanThe alteration of intestinal mucosal barrier is considered to be the central pathophysiological process in response to gastrointestinal infections, and mucosal microstructural damage is a major factor for enhancing epithelial permeability in persistent bacterial infections. However, the mechanism involved in hyperpermeability in the early stage of acute bacterial infections is not fully understood. In the present study, fluorescein isothiocyanate-dextran across and transepithelial resistance measured in Ussing chambers were used to assess the intestinal paracellular permeability. Mast cell activation was evaluated by western blotting for the presence of tryptase released from mast cells. Serum levels of interleukin-6 were evaluated using enzymelinked immunosorbent assay. Our results indicated that mast cells played a pivotal role in colonic mucosal hyperpermeability in wild type mice treated with lipopolysaccharide (LPS) for 2 h. And the effect of LPS was mainly dependent on mast cell degranulation, while no change in permeability was observed in the mast celldeficient mice (Wads-/- ) after LPS administration. No obvious changes of the mucosal structure including histomorphological architecture and expression of intercellular junction proteins were obtained in either wild type or Wads-/- mice after LPS stimulation by hematoxylin and eosin staining, immunofluorescence staining and western blot analysis. Furthermore, the selfrenewal of intestinal epithelia, detected by using proliferation marker 5’-bromo-2’-deoxyuridine, was not involved in increased permeability. Collectively, activation of mast cells induced by LPS mediated intestinal hyperpermeability in the initial stage, and played a crucial role in barrier dysfunction rather than mucosal microstructural damage in acute enterogenous bacterial infection.
- PublicationOpen AccessCO2 laser therapy accelerates the healing of ulcers in the oral mucosa by inducing the expressions of heat shock protein-70 and tenascin C(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Gang, Luo; Oka, Kyoko; Ohki, Shirabe; Rikitake, Mihoko; Itaya, Satoshi; Tamura, Shougo; Toda Nakamura, Masako; Ogata, Kayoko; Kira Tatsuok, Michiko a; Ozaki, MasaoThe treatment of ulceration or stomatitis with laser therapy is known to accelerate healing and relieve pain, but the underlying biological mechanism is not fully understood. The present study used a mouse model of ulceration to investigate the molecular mechanisms by which CO2 laser therapy accelerated the wound healing process. An ulcer was experimentally created in the palatal mucosa of the mouse and irradiated with light from a CO2 laser. Compared with controls (no irradiation), laser irradiation induced the proliferation of epithelial cells and faster re-epithelialization of the wound area. Immunohistochemistry experiments showed that heat shock protein-70 (HSP70) was expressed mainly in the epithelium of normal palatal tissue, whereas there was little tenascin C (TnC) expression in the epithelium and mesenchyme under normal conditions. Laser irradiation induced HSP70 mRNA and protein expression in the lamina propria as well as TnC expression in the mesenchyme underlying the renewing epithelium. Epithelial cells and fibroblasts were exposed to heated culture medium or laser irradiation to establish whether hyperthermia mimicked the effect of laser irradiation. Culture of fibroblasts in heated medium increased the expressions of both TnC and TGF-β1, whereas laser irradiation induced only TnC expression. The present study indicates that CO2 laser irradiation exerts a photobiogenic effect to up-regulate TnC expression without inducing TGF-β1 expression. We suggest that CO2 laser therapy has an advantage over thermal stimulation.
- PublicationOpen AccessInterleukin-6: a molecule with complex biological impact in cancer(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Lacina, Lukáš; Brábek, Jan; Král, Vladimír; Kodet, Ondřej; Smetana Jr, KarelInterleukin-6 is a multifaceted cytokine, usually reported as a pro-inflammatory molecule. However, certain anti-inflammatory activities were also attributed to IL-6. The levels of IL-6 in serum as well as in other biological fluids are elevated in an agedependent manner. Notably, it is consistently reported also as a key feature of the senescence-associated secretory phenotype. In the elderly, this cytokine participates in the initiation of catabolism resulting in, e.g. sarcopenia. It can cross the blood-brain barrier, and so it is in causal association with, e.g. depression, bipolar disorder, schizophrenia, and anorexia. In the cancer patient, IL-6 is produced by cancer and stromal cells and actively participates in their crosstalk. IL-6 supports tumour growth and metastasising in terminal patients, and it significantly engages in cancer cachexia (including anorexia) and depression associated with malignancy. The pharmacological treatment impairing IL-6 signalling represents a potential mechanism of antitumour therapy targeting cancer growth, metastatic spread, metabolic deterioration and terminal cachexia in patients.