Histology and histopathology Vol. 5, nº 3 (1990)

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https://hdl.handle.net/10201/17697

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Serotonin-producing pancreatic endocrine tumour. Histological, ultrastructural and immunohistochemical study of a case
(Murcia : F. Hernández, 1990) Kanavaros, Panagiotis; Hoang, Catherine; Le Bodic, Marie Francoise; Polivka, Marc; Hautefeuille, Pierre
Serotonin-producing pancreatic endocrine tumours are rare neoplasms which in most cases exhibit malignant biological behaviour. These tumours, in the majority of the well-documented cases, are composed of argyrophil- and argentaffin-positive cells which contain large pleomorphic neurosecretory granules. In contrast, argyrophilic non-argentaffin pancreatic endocrine tumours with tumour cells containing round neurosecretory granules are exceptional. In this study we describe such a tumour not associated with clinical evidence of carcinoid syndrome in a 60-year-old woman. Histological examination revealed tumour extension in pancreatic lymphatic vessels and veins but no evidence of locoregional or distant metastases. Ten months after surgery the patient showed no recurrence of the disease. Immunohistochernistry revealed cytoplasmic serotonin production in the tumour cells which were negative for anti-gastrin, insulin, glucagon, somatostatin, pancreatic polypeptide (PP), vasoactive intestinal peptide (VIP) and ACTH. This study emphasizes the usefulness of combined ultrastructural and immunohistochemical investigations in order to identify and characterize the rare pancreatic endocrine tumours with serotonin production.
Open Access
The ruputure spleen. A hisological, morphometrical an immunohistochemical study
(Murcia : F. Hernández, 1990) Barnard, H.; Dreef, E.J.; van Krieken, J.H.J.M.
A traumatically ruptured spleen is regarded as a proper control in many histological and immunological studies on the human spleen. This paper compares spleens that ruptured due to trauma and spleens which were removed during surgery in patients without splenic pathology. Based on a histological, morphometrical, and immunohistochemcial description of the control spleens it is shown that the traumatically ruptured spleens contain alterations in the lymphoid tissue. The amount of white pulp is increased due to a larger amount of CD4- positive lymphocytes. Furthermore there are alterations in lymphocyte populations in the different splenic compartments. It is concluded that spleens that rupture may be predisposed due to immunological stimulation.
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Ground-glass hepatocytes. ligth and electron microscopy. Characterization of the different types
(Murcia : F. Hernández, 1990) Jaime Vázquez, J.
Morphological observations of paraffinembedded histological sections stained with H&E led to the discovery of some cytoplasmic changes which occur in different conditions, although they look alike under the light microscope. These hepatocytic changes consisted basically of homogeneous areas which are weakly eosinophilic in H & E-stained sections. They are frequently referred to as «inclusion» bodies, even when they are not true inclusions. The hepatocytic changes obsewed in HBsAg carriers, in chronic alcoholic patients treated with cyanamide to discourage them from drinking alcohol, in Lafora's disease, and in glycogenosis type IV, look very similar in paraffin sections stained with H&E. Nevertheless, they can be differentiated using ancillary techniques. On electron microscopy they do not look alike. Of particular interest are the «inclusion» bodies induced by cyanamide, a predictable and reproductible lesion, which in man eventually leads to cirrhosis. Other types of hepatocytic changes also giving a rather vague eground-glass» appearance to the cytoplasm are those resulting from intracytoplasmic accumulation of proteins, particularly fibrinogen, and those observed in patients treated with different drugs.
Open Access
Studies with the Golgi method in central gangliogliomas and dysplastic gangliocytoma of the cerebellum (Lhermitte-Duclos disease)
(Murcia : F. Hernández, 1990) Ferrer, I.; Martí, E.; Guionneti, N.; Bella, R.; Serrano, T.; Towse, J.; Conesa, G.; Isamat, F.
The rapid Golgi method, combined with current optical and electronmicroscopica1 techniques, was used in three central gangliogliomas and in one dysplastic gangliocytoma of the cerebellum to study the morphology of ganglionic cells. Gangliogliomas were composed of bipolar, fusiform and radiate cells with dense core and clear vesicles in the perikaryon and cellular processes, the number of each cellular type varying from one case to another. These features, together with the fact that isodendritic neurons are considered to be phylogenetically old neurons, suggest that these tumours are composed of «primitive» neurons that are not homogeneous with regard to their morphology. In contrast, ganglionic cells in dysplastic gangliocytoma are huge cells with long, stereotyped neurites that establish unique asymmetric contacts with neighbouring perikarya and neurites by means of claw-shaped processes covered with synaptic buttons. These morphological characteristics are different from those of any other neuron of the CNS.
Open Access
The autonomic innervation of the liver and gallbladder of Podarcis hispanica
(Murcia : F. Hernández, 1990) Azanza, M.J.; Aisa, J.; Junquera, C.
The innervation of the liver and gallbladder of the lizard Podarcis hispanica has been studied by the following methods: a) demonstration of cholinesterase activity; b) FIF method for catecholamines; and c) immunohistochemistry for VIP. The hepatic parenchyma of the reptile's liver show hzpatocytes arranged in regular rows of hepatic cords, the portal triad being typical of higher vertebrates (birds and mammals). Nerve fibers are found in the scarce connective tissue distributed among the hepatocytes. The innervation is restricted to the big branches of blood vessels and biliary ducts. It is represented by cholinergic, noradrenergic and VIPergic fibers. The gallbladder shows a well developed cholinergic plexus with pyramidal cells in the interconnection points of the fiber network. The noradrenergic and VIPergic plexuses are also more widely distributed in the gallbladder thali in the liver.

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