Histology and histopathology Vol. 1, nº 3 (1986)

Ir a Estadísticas

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https://hdl.handle.net/10201/17681

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Observations on cell kinetics and viability of a human melanoma cell line exposed to dicarboxylic acids in tissue culture
(Murcia : F. Hernández, 1986) Breathnach, A. S.; Robins, E.J.; Bhasin, Y. P.; Ethridge, L. B.; Nazzaro-Porro, M.; Passi, S.; Picardo, M.
Cultures of human nlelanonla cell line BOO08 \verc e-iposed to the disodium salts of arelaic acid (C,, 2Na), adipic acid (C,, 2Na) and dodecanediaic acid (C,, 2Na) at IO'M and 5 x 10-'M for 24 hrs. None of the diacid salts had a significant effect on growth rate or viability of the cells, at 10-'M for 24 hrs nor had C, 2Na any effect at 5 x 10'M. At 5 x 102M for 24 hrs, both C,, 2Na, and CIL2 Na had a significant effect in reducing both growth and viability. These effects were accompanied by morphological evidence of cell death, and swelling of mitochondria and accuni~~latioonf lipid droplets within cytoplasm of still biable cells.
Open Access
Effects of mellitic acid (MA) and sodium fluoride (NaF) on the histological appearance of murine fetal tibiae cultured in vitro
(Murcia : F. Hernández, 1986) Weischer, C.H.; Krisinger, J.; Karzel, K.
The aim of this studv was to develop a standardized image analysis method for localization and quantitative measurement of calcified structures of murine fetal tibiae cultured in vitro as a completion and verification of previous biochemical studies. The calcified structures of bone stained by von Kossa silver technique and the epiphyseal cartilages showing intensive metachromasia with toluidine-blue staining were converted with grey-value window programs and afterwards the areas of the selected structures were measured. The histomorphological investigations showed that the murine tibiae, incubated for a period of 6 days in a medium with addition of 5 mmol mellitic acid, showed both a significant reduction of calcium deposits and an increase of epiphyseal intercellular cartilage matrix. The tibiae incubated in a medium with addition of 0.5 mmol sodium fluoride significantly showed an increase of calcium deposits in the thickened lammellae of the cornpacta. These histomorphological results confirm previous biochemical studies.
Open Access
Non Hodgkin's lymphomas following chemoradiotherapy for Hodgkin's disease. Two new cases and a review of the literature
(Murcia : F. Hernández, 1986) Marigil, Miguel A.; Pardo-Mindan, F.J.; Cuesta, B.; Rocha, E.; Idoate, M.A.; Gomez-Bezares, M.C.
Two patients developed non-Hodgkin's lymphoma (NHL) six and ten years after radiotherapy and chemotherapy for Hodgkin's disease nodular sclerosis type. The histological classification of the developing NHL for the two patients was: IgG (K) secreting lymphoplasmacytoid lyn~phomao f the stomach, and immunoblastic lymphoma of [he cervical lymph nodes. Both patients responded well lo conventional chen~otherapyf or NHL and are alive 22 and 5 months after the diagnosis of the secondary tumor. Forty eight cases of NHL after treatment for HD have been previously reported. We present a review of the literature , of these cases, adding to this literature the first reported case of gastric lyniphoplasmacytoid lymphoma under such circumstances.
Open Access
The kinetic differences between sodium nitrite, amyl nitrite and nitroglycerin oxidation of hemoglobin
(Murcia : F. Hernández, 1986) Tarburton, John Philip; Metcalf, William K.
The effect of sodium nitrite, amyl nitrite and nitroglycerin (glyceryl trinitrate) on the hemoglobin of adult erythrocytes was examined in vitro. Both amyl nitrite and nitroglycerin reacted immediately with oxyhemoglobin to effect oxidation into methemoglobin while sodium nitrite required an inductionary period (lag phase) prior to the reaction. Kinetic studies of the biniolecular rate law for each of the preceeding reaction's reactionary periods (log phases) allowed rate constant calculations to be made. The values are 1.14 x 10JM niin I, 7.45 x IOJbl lmin I , and 3.50 x 10IM ' m i n 1 for sodium nitrite, amyl nitrite and nitroglycerin, respectic ely. A comparison of the amyl nitrite and nitroglycerin rate constants reveals that aniyl nitrite is approximately 2000-fold more toxic to oxyhemoglobin than nitroglycerin. These oxidant's effect on in !/itro hemoglobin solutions are comparable since both reactions approximate to rectangular hyperbolae. Sodium nitrite reacts about 300-fold faster with oxyhemoglobin than does nitroglycerin. However, the sodium nitrite reaction proceeds in a sigmoidal fashion which makes a strict comparison between these compounds relative toxicities less clear cut.
Open Access
lmmunohistochemical reaction of myocardial fibers with actin antiserum in autopsy cases of myocardial infarction
(Murcia : F. Hernández, 1986) Shigeo Hashimoto; Shozo Nishida; Shingo Hiruma; Manabu Takahashi; Yoshiki Enomoto; Kuniyasu Sakatani
The purpose of this study was to determine the immunoreactivity of myocardial actin filaments with actin antiserum and to examine the significance of its application to diseased human cardiac muscle. The actin was extracted and purified from chicken gizzards. Anti-actin rabbit serum was prepared and purified by affinity chromatography and defined by an immunoblotting test. Using the avidin-biotin-peroxidase complex (ABC) method, the actin antiserum was applied to paraffin sections prepared from hearts taken from routine autopsies of patients who had died of myocardial infarction. Reactivity was shown to be completely lost, not only in necrotized fibers, but also in non-specific degenerative fibers which could be identified by their eosinophilic cytoplasm with pyknotic nuclei, and clearly remaining and/or diminished cross-striations stained with hematoxylin-eosin. In contrast, hypertrophic myocardial fibers adjacent to granulation or scar tissue and those adjacent to infarcted foci exhibited a more intense reaction. These results indicated that the immunohistochemical reaction of actin filaments can be used for the easy detection of very mild degrees of degeneration of cardial muscle fibers, and for hypertrophic fibers adjacent to diseased foci. Studies of the immunoreactivity of actin protein suggestive of alteration at the molecular level might yield morphological clues regarding the nature of functional activity in the contraction of cardiac fibers.

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