Histology and histopathology Vol. 1, nº 3 (1986)

Ir a Estadísticas

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    Observations on cell kinetics and viability of a human melanoma cell line exposed to dicarboxylic acids in tissue culture
    (Murcia : F. Hernández, 1986) Breathnach, A. S.; Robins, E.J.; Bhasin, Y. P.; Ethridge, L. B.; Nazzaro-Porro, M.; Passi, S.; Picardo, M.
    Cultures of human nlelanonla cell line BOO08 \verc e-iposed to the disodium salts of arelaic acid (C,, 2Na), adipic acid (C,, 2Na) and dodecanediaic acid (C,, 2Na) at IO'M and 5 x 10-'M for 24 hrs. None of the diacid salts had a significant effect on growth rate or viability of the cells, at 10-'M for 24 hrs nor had C, 2Na any effect at 5 x 10'M. At 5 x 102M for 24 hrs, both C,, 2Na, and CIL2 Na had a significant effect in reducing both growth and viability. These effects were accompanied by morphological evidence of cell death, and swelling of mitochondria and accuni~~latioonf lipid droplets within cytoplasm of still biable cells.
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    Effects of mellitic acid (MA) and sodium fluoride (NaF) on the histological appearance of murine fetal tibiae cultured in vitro
    (Murcia : F. Hernández, 1986) Weischer, C.H.; Krisinger, J.; Karzel, K.
    The aim of this studv was to develop a standardized image analysis method for localization and quantitative measurement of calcified structures of murine fetal tibiae cultured in vitro as a completion and verification of previous biochemical studies. The calcified structures of bone stained by von Kossa silver technique and the epiphyseal cartilages showing intensive metachromasia with toluidine-blue staining were converted with grey-value window programs and afterwards the areas of the selected structures were measured. The histomorphological investigations showed that the murine tibiae, incubated for a period of 6 days in a medium with addition of 5 mmol mellitic acid, showed both a significant reduction of calcium deposits and an increase of epiphyseal intercellular cartilage matrix. The tibiae incubated in a medium with addition of 0.5 mmol sodium fluoride significantly showed an increase of calcium deposits in the thickened lammellae of the cornpacta. These histomorphological results confirm previous biochemical studies.
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    Non Hodgkin's lymphomas following chemoradiotherapy for Hodgkin's disease. Two new cases and a review of the literature
    (Murcia : F. Hernández, 1986) Marigil, Miguel A.; Pardo-Mindan, F.J.; Cuesta, B.; Rocha, E.; Idoate, M.A.; Gomez-Bezares, M.C.
    Two patients developed non-Hodgkin's lymphoma (NHL) six and ten years after radiotherapy and chemotherapy for Hodgkin's disease nodular sclerosis type. The histological classification of the developing NHL for the two patients was: IgG (K) secreting lymphoplasmacytoid lyn~phomao f the stomach, and immunoblastic lymphoma of [he cervical lymph nodes. Both patients responded well lo conventional chen~otherapyf or NHL and are alive 22 and 5 months after the diagnosis of the secondary tumor. Forty eight cases of NHL after treatment for HD have been previously reported. We present a review of the literature , of these cases, adding to this literature the first reported case of gastric lyniphoplasmacytoid lymphoma under such circumstances.
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    Substance P-like immunoreactivity in rat and cat carotid bodies, Light and electron microscopic studies
    (Murcia : F. Hernández, 1986) I-li Chen; Yates, Robert D.; Hansen, John T.
    Substance P-immunoreactive (SP-I) structures in the carotid bodies of rats and cats were examined with the light and electron microscopes. In both species SP-I varicose nerve fibers were located singly in the interstitial connective tissue in close association with blood vessels. They were small unmyelinated fibers enveloped in a common Schwann cell sheath with other SP-negative fibers. Some of SP-I fibers contained large dense-cored granules and small clear vesicles in addition to microtubules and mitochondria and probably represented nerve fiber varicosities. The latter often were found incompletely invested by Schwann cell sheaths. SP-fibers were found occasionally in the envelopes of supporting cells at the periphery of parenchymal cell groups. However, none of the nerve terminals making synaptic contacts with glomus cells exhibited SP-like immunoreactivity. In cat carotid bodies some glomus cells showed moderate to intense SPlike immunoreactivity.The intense SP-I glomus cells displayed numerous dense-cored vesicles of 85 to 140 nm in diameter and frequently showed synaptic contacts with SP-negative nerve terminals. In rat carotid bodies we were unable to detect consistent SP-immunoreactivity in glomus cells. Our results do not favor the hypothesis that SP is a neurotransmitter/modulator in the chemoreceptor afferents synapsing on glomus cells in either the cat or rat carotid body. However our results support the hypothesis that SP in cat glomus cells may play a role in the modulation of chemoreceptor activity.
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    The kinetic differences between sodium nitrite, amyl nitrite and nitroglycerin oxidation of hemoglobin
    (Murcia : F. Hernández, 1986) Tarburton, John Philip; Metcalf, William K.
    The effect of sodium nitrite, amyl nitrite and nitroglycerin (glyceryl trinitrate) on the hemoglobin of adult erythrocytes was examined in vitro. Both amyl nitrite and nitroglycerin reacted immediately with oxyhemoglobin to effect oxidation into methemoglobin while sodium nitrite required an inductionary period (lag phase) prior to the reaction. Kinetic studies of the biniolecular rate law for each of the preceeding reaction's reactionary periods (log phases) allowed rate constant calculations to be made. The values are 1.14 x 10JM niin I, 7.45 x IOJbl lmin I , and 3.50 x 10IM ' m i n 1 for sodium nitrite, amyl nitrite and nitroglycerin, respectic ely. A comparison of the amyl nitrite and nitroglycerin rate constants reveals that aniyl nitrite is approximately 2000-fold more toxic to oxyhemoglobin than nitroglycerin. These oxidant's effect on in !/itro hemoglobin solutions are comparable since both reactions approximate to rectangular hyperbolae. Sodium nitrite reacts about 300-fold faster with oxyhemoglobin than does nitroglycerin. However, the sodium nitrite reaction proceeds in a sigmoidal fashion which makes a strict comparison between these compounds relative toxicities less clear cut.