Browsing by Subject "Octreotide"

Now showing 1 - 3 of 3
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    Effect of octreotide on expression of calcitonin gene in cultured cells of medullary thyroid carcinoma
    (Murcia : F. Hernández, 2004) Zabel, M.
    The studies were performed on cultured TT cells, originating from medullary thyroid carcinoma and producing calcitonin (CT) and CT gene-related peptide (CGRP), synthesized on the template of the same gene. Addition of octreotide (a synthetic somatostatin analogue) to the cell culture augmented amounts of CGRP mRNA and of CGRP protein in the cells as well as increased levels of CGRP in the culture medium. In contrast, amounts of CT mRNA and of CT protein in the cells as well as levels of CT in the medium remained unchanged. The results showed that octreotide stimulates transcription and alternate splicing of the CT gene.
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    Effects of octreotide on propylotiouracil-induced goiter in rats: a quantitative evaluation
    (Murcia : F. Hernández, 1998) Pawlikowski, M.; Zielinski, K.; Slowinska-Klencka, D.; Klencki, M.
    To evaluate the possible antigoitrogenic effect of somatostatin, the influence of long-acting somatostatin analog - octreotide - on experimental goiter developed in rats treated with propylthiouracil was examined. Goiter formation was assessed by measurement of the main histological compartments of the thyroid as well as by morphometric analysis of the vascularization and blood supply of the gland. Although treatment with octreotide did not prevent the goiter formation, it clearly reduced blood supply and vascularization of the thyroid and counteracted propylthiouracil- induced increase in the relative volume of follicular epithelium. To conclude, the somatostatin analog - octreotide - is effective in reduction of goiter vascularisation. This finding provides a rationale for the clinical trials of the treatment of hypewascular goiter by somatostatin analogs.
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    The effect of somatostatin analog octreotide on diethylstilbestrol-induced prolactin secretion, cell proliferation and vascular changes in the rat anterior pituitary gland
    (Murcia : F. Hernández, 1997) Pawlikowski, M.; Kunert-Radek, J.; Grochal, M.; Zielinski, K.; Kulig, Andrzej
    The effects of diethylstilbestrol (DES) and of long-acting somatostatin analog, octreotide (SMS) on the rat anterior pituitary microvasculature have been studied by means of computer-assisted image analysis. Additionally, the effects of DES and SMS on prolactin secretion and anterior pituitary cell proliferation have been studied, as well. The vascularization was visualized using Selye's method modified by Poely et al. (1964). The prolactin serum levels were estimated by radioimmunoassay. The proliferation indices were assessed using bromodeoxyuridine incorporation assay. As expected, it was found that DES sharply increased serum prolactin levels and enhanced cell proliferation in the anterior pituitary gland. DES also induced changes in parameters of vascularization. Simultaneous treatment of rats with SMS inhibited the DES-induced elevation of prolactin levels and pituitary cell proliferation. It also suppressed some but not al1 DES-induced changes in the anterior pituitary vascularization. These data suggest that the angio-inhibitory activity of SMS might be involved in its anti-tumor action on pituitary adenomas, but not as a sole or principal mechanism.