Publication: Cyclodextrins increase triterpene production in Solanum lycopersicum cell cultures by activating biosynthetic genes
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Date
2022-10-20
Authors
Sabater Jara, Ana Belén ; Marín Marín, María Jesús ; Almagro, Lorena ; Pedreño, María Angeles
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Publisher
MDPI
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Description
© 2022 by the authors. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/
This document is the Published Manuscript version of a Published Work that appeared in final form in Plants. To access the final edited and published work see https://doi.org/10.3390/plants11202782
Abstract
In this work, Solanum lycopersicum cv. Micro-Tom suspension-cultured cells were used
to analyze the effect of different elicitors including β-cyclodextrins (CD), methyl jasmonate (MJ),
β-glucan (Glu) and 3-hexenol (Hex) separately and the combined treatments of CD + MJ, CD + glu
and CD + Hex on triterpene compound production after 24, 72 and 96 h. Moreover, we studied the
changes induced by elicitors in the expression of key biosynthetic genes to elucidate the regulation of
the triterpene biosynthetic pathway. The relative abundance of the triterpene compounds identified in
the extracellular medium after elicitation (squalene, fucosterol, avenasterol, β-sitosterol, cycloartenol
and taraxasterol) was determined by gas chromatography coupled to mass spectrometry, and the
expression level of genes in treated-cells was analyzed by real-time quantitative polymerase chain
reaction (qRT-PCR). Results showed that, in CD-treated cells (CD, CD + MJ, CD + Glu, CD + Hex),
specialized metabolites were accumulated mainly in the extracellular medium after 72 h of elicitation.
Moreover, qRT-PCR analysis revealed that the highest triterpene levels in CD-treated cells (CD,
CD + MJ, CD + Glu, CD + Hex) were highly correlated with the expression of cycloartenol synthase,
3-hydroxy-3-methylglutaryl-CoA reductase and squalene epoxidase genes at 24 h of treatment, whereas
the expression of sterol methyltransferase was increased at 72 h. According to our findings, CD acts as
a true elicitor of triterpene biosynthesis and can promote the release of bioactive compounds from
the tomato cells into the extracellular medium. The results obtained provide new insights into the
regulation of the triterpene metabolic pathway, which might be useful for implementing metabolic
engineering techniques in tomato.
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