Publication: Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin
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Date
1998
Authors
Brorson, S. H.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Considering the importance of immunolocalization of cellular substances combined with good
ultrastructure and ease of use , this review is focused on
the use of resin and the possibilities of manipulating the
resin before and after embedding in order to improve the
immunolabeling of resin sections for electron
microscopy. The qualities of acrylic re sin s and
conventional epoxy resin for immunoelectron microscopy are discussed. Acrylic sections are usually more
suited for immunoelectron microscopy than conventional epoxy sections. Different etching procedures
(sodium ethoxide or sodium metaperiodate) may be
applied to conventional epoxy sections to enhance the
yield of immunolabeling. Lately, a method which does
not involve any kind of etching has been developed for
enhancing the immunogold labeling of epoxy sections
up to about 8 times. This method involves increased
concentration of accelerator in the epoxy resin mixture
when processing the tissue. The ultrastructural
preservation of the tissue is important in immunoelectron microscopical procedures. and not only the
intensity of the immunolabeling: in this respect no resin
may compete with the widely used epoxy resins.
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