Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin

Brorson, S. H.

Publication:
Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin

dc.contributor.author	Brorson, S. H.
dc.date.accessioned	2020-05-22T14:27:51Z
dc.date.available	2020-05-22T14:27:51Z
dc.date.issued	1998
dc.description.abstract	Considering the importance of immunolocalization of cellular substances combined with good ultrastructure and ease of use , this review is focused on the use of resin and the possibilities of manipulating the resin before and after embedding in order to improve the immunolabeling of resin sections for electron microscopy. The qualities of acrylic re sin s and conventional epoxy resin for immunoelectron microscopy are discussed. Acrylic sections are usually more suited for immunoelectron microscopy than conventional epoxy sections. Different etching procedures (sodium ethoxide or sodium metaperiodate) may be applied to conventional epoxy sections to enhance the yield of immunolabeling. Lately, a method which does not involve any kind of etching has been developed for enhancing the immunogold labeling of epoxy sections up to about 8 times. This method involves increased concentration of accelerator in the epoxy resin mixture when processing the tissue. The ultrastructural preservation of the tissue is important in immunoelectron microscopical procedures. and not only the intensity of the immunolabeling: in this respect no resin may compete with the widely used epoxy resins.	es
dc.format	application/pdf	es
dc.format.extent	7	es
dc.identifier.eisbn	Histology and histopathology, Vol. 13, nº 1 (1998)	es
dc.identifier.issn	1699-5848
dc.identifier.issn	0213-3911
dc.identifier.uri	http://hdl.handle.net/10201/92546
dc.language	eng	es
dc.publisher	F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 International	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Acrylic resin	es
dc.subject	LR-White	es
dc.subject	Epoxy resin	es
dc.subject	Immunogold	es
dc.subject.other	CDU::6 - Ciencias aplicadas::61 - Medicina	es
dc.title	Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es

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