Browsing by Subject "Immunogold"

Now showing 1 - 3 of 3
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    Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin
    (F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología, 1998) Brorson, S. H.
    Considering the importance of immunolocalization of cellular substances combined with good ultrastructure and ease of use , this review is focused on the use of resin and the possibilities of manipulating the resin before and after embedding in order to improve the immunolabeling of resin sections for electron microscopy. The qualities of acrylic re sin s and conventional epoxy resin for immunoelectron microscopy are discussed. Acrylic sections are usually more suited for immunoelectron microscopy than conventional epoxy sections. Different etching procedures (sodium ethoxide or sodium metaperiodate) may be applied to conventional epoxy sections to enhance the yield of immunolabeling. Lately, a method which does not involve any kind of etching has been developed for enhancing the immunogold labeling of epoxy sections up to about 8 times. This method involves increased concentration of accelerator in the epoxy resin mixture when processing the tissue. The ultrastructural preservation of the tissue is important in immunoelectron microscopical procedures. and not only the intensity of the immunolabeling: in this respect no resin may compete with the widely used epoxy resins.
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    lmmunogold identification of the GH cells of goat in different physiological conditions
    (Murcia : F. Hernández, 1993) Sánchez, J.; Navarro, J. A.; Bernabé, A.; Serrano, J.; Gómez, S.
    Growth hormone-containing cells (GH cells) in the anterior pituitary gland of the female goat during anoestrus, pregnancy and lactation were identified by the immunogold complex technique combined with electron microscopy. In the three physiological stages analyzed, most of the GH cells were characterized by a round to oval or triangular shape and the presence of numerous spherical and electrondense secretory granules (300- 1,200 nm). Granules with the largest diameters were observed during the lactating stage. In these cells, the rough endoplasmic reticulum (RER) and Golgi complex (GC) were usually poorly developed. Also in lactating animals, a low number of GH cells with more highly developed RER and GC with a few small secretory granules (230-360 nm in diameter) were also observed. This structural configuration suggests that these cells are more active than those with a lower development of cytoplasmic organelles, although their low frequency does not support a considerable increase in hormonal synthesis and release during lactation in comparison with anoestrus and pregnancy stages.
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    Morphologic and immunoelectronmicroscopic identification of human T cell lymphotropic virus type III (HTLV-III).
    (Murcia : F. Hernández, 1986) Tirnár, J.; Nagy, K.; Lapis, K.
    The AIDS associated HTLV-111 virus infected H9 cells were extensively studied using light, scanning and transmission electronmicroscopy. It was demonstrated that the morphological features of HTLV-111 are different from the C-type particles and are similar to those of lentiviridae. For immunological identification high titer pre-AIDS patient sera served as the anti-HTLV-111 envelope antibody source. The immunoelectronmicroscopic method was able to identify the viral envelope antigen on the surface of infected cells and in certain areas of the viral envelope. This is the first application of immunoelectronmicroscopy for the identification of the HTLV-111 virus