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An ideal spacing is required for the control of Class II CRP-dependent promoters by the status of CRP K100.

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dc.archivorevisado©2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Submitted, Accepted, Published, version of a Published Work that appeared in final form in FEMS Microbiology Letters. To access the final edited and published work see doi:10.1093/femsle/fnaa164
dc.contributor.authorÉcija Conesa, Ana
dc.contributor.authorLozano Terol, Gema
dc.contributor.authorBrowning, Douglas F.
dc.contributor.authorBusby, Steve J. W.
dc.contributor.authorWolfe, Alan J.
dc.contributor.authorCánovas Díaz, Manuel
dc.contributor.authorDiego Puente, Teresa de
dc.contributor.authorGallego Jara, Julia
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.date.accessioned2023-12-12T23:11:55Z
dc.date.available2023-12-12T23:11:55Z
dc.date.issued2020
dc.description.abstractTranscription activation by the Escherichia coli CRP at Class II promoters is dependent on direct interactions between RNA polymerase and CRP, therefore the spatial proximity between both proteins plays a significant role in the ability of CRP to activate transcription. Using both in vivo and in vitro techniques, here we demonstrate that the CRP K100 positive charge, adjacent to AR2, is required for full promoter activity when CRP is optimally positioned. Accordingly, K100 mediated activation is very position-dependent and our data confirm that the largest impact of the K100 status on transcription activation occurs when the spacing between the CRP binding site and the A2 of the −10 element is 22 bp. From the results of this study and the progress in the understanding about open complex DNA scrunching, we propose that CRP-dependent promoters should now be numbered by the distance from the center of the DNA site for CRP and the most highly conserved base at position 2 of the −10 hexamer in bacterial promoters
dc.formatapplication/pdfes_ES
dc.format.extent9
dc.identifier.citationFEMS Microbiology Letters, 367, 2020, fnaa164
dc.identifier.doi10.1093/femsle/fnaa164
dc.identifier.issn0378-1097
dc.identifier.urihttp://hdl.handle.net/10201/136579
dc.languageenges_ES
dc.publisherOxford Academic
dc.relationSin financiación externa a la Universidad
dc.relation.isreferencedbyED_IDENTRADA=1270
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEscherichia coli
dc.subjectCyclic AMP receptor protein (CRP)
dc.subjectTranscription activation
dc.subjectClass II CRP-dependent promoters
dc.titleAn ideal spacing is required for the control of Class II CRP-dependent promoters by the status of CRP K100.es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dspace.entity.typePublicationes
relation.isAuthorOfPublication57bf3537-1124-49ab-8774-bd0c3bdf1c28
relation.isAuthorOfPublicationb8be181a-7224-475a-8427-543751ef593c
relation.isAuthorOfPublication0212fc0f-e15c-4b27-9a2d-116768a47044
relation.isAuthorOfPublication.latestForDiscovery57bf3537-1124-49ab-8774-bd0c3bdf1c28
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