Publication: An ideal spacing is required for the control of Class II CRP-dependent promoters by the status of CRP K100.
Authors
Écija Conesa, Ana ; Lozano Terol, Gema ; Browning, Douglas F. ; Busby, Steve J. W. ; Wolfe, Alan J. ; Cánovas Díaz, Manuel ; Diego Puente, Teresa de ; Gallego Jara, Julia
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Publisher
Oxford Academic
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DOI
10.1093/femsle/fnaa164
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info:eu-repo/semantics/article
Description
Abstract
Transcription activation by the Escherichia coli CRP at Class II promoters is dependent on direct interactions between RNA
polymerase and CRP, therefore the spatial proximity between both proteins plays a significant role in the ability of CRP to
activate transcription. Using both in vivo and in vitro techniques, here we demonstrate that the CRP K100 positive charge,
adjacent to AR2, is required for full promoter activity when CRP is optimally positioned. Accordingly, K100 mediated
activation is very position-dependent and our data confirm that the largest impact of the K100 status on transcription
activation occurs when the spacing between the CRP binding site and the A2 of the −10 element is 22 bp. From the results
of this study and the progress in the understanding about open complex DNA scrunching, we propose that CRP-dependent
promoters should now be numbered by the distance from the center of the DNA site for CRP and the most highly conserved
base at position 2 of the −10 hexamer in bacterial promoters
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Citation
FEMS Microbiology Letters, 367, 2020, fnaa164
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