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Chemocytoarchitecture of the rat locus ceruleus

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dc.contributor.authorLijima, K.es
dc.date.accessioned2011-02-01T09:17:03Z
dc.date.available2011-02-01T09:17:03Z
dc.date.issued1993
dc.description.abstractI shall elaborate on the cytochemical classifications of the rat locus ceruleus (LC) and state how each of these could be identified. In the LC, 80% ( 1,30911,642) of the noradrenergic (NA) neurons are also GABAergic. This is found by demonstrating that two immunoreactivities coexist in adjacent sections alternately immunostained by anti-TH or anti-GABA antibody. Pharmacological manipulations with pargyline (75 mglkg, i.p., 2 h prior to perfusion) and L-tryptophan (1 50 mglkg, 1.5 h prior to perfusion) revealed 5-HT-like immunoreactivity (5-HT-LI) in most LC cells (masked 5-HT cells) that produce 5-HT but not other indoleamines. If 5-HTP is applied instead of tryptophan, 5-HT-L1 is shown by the raphe nuclei and a few LC cells (masked indoleamine cells) in the marginal zone. Anti- GAD antibody reveals GAD-L1 in 32% of GABA neurons predominantly in the dorsal division. In situ hybridization studies detected tryptophan hydroxylase mRNA and GAD mRNA in many small- and mediumsized neurons. It is concluded that the LC consists mostly of an NA population that is possibly synthesizing multiple transmitters, such as GABA, GAD and 5-HT in single neurons so that the system enables the LC simultaneously to innervate the entire CNS.es
dc.formatapplication/pdfes
dc.format.extent11es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/18434
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectLocus ceruleuses
dc.subjectImmunocytochemistryes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleChemocytoarchitecture of the rat locus ceruleuses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol. 8, nº 3 (1993)