Publication: Alterations in haemolymph proteome of Mytilus galloprovincialis mussel after an induced injury
Authors
Franco-Martínez, Lorena ; Martínez-Subiela, Silvia ; Escribano, Damián ; Schlosser, Sarah ; Nöbauer, Katharina ; Razzazi-Fazeli, Ebrahím ; Romero, Diego ; Cerón, José Joaquín Cerón ; Tvarijonaviciute, Asta
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.fsi.2018.01.038
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info:eu-repo/semantics/article
Description
©2018 Elsevier Ltd. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Fish and Shellfish Immunology. To access the final edited and published work see https://doi.org/10.1016/j.fsi.2018.01.038
Abstract
A proteomic and biochemical approach was performed to assess the effects of an induced muscle injury on the
haemolymph of bivalve molluscs. For this purpose, Mytilus galloprovincialis were exposed to puncture of adductor muscle for three consecutive days, and their haemolymph proteome was then compared to healthy animals using 2-dimensional electrophoresis (2-DE) to identify proteins that differed significantly in abundance. Those proteins were then subjected to tandem mass spectrometry and 6 proteins, namely myosin, tropomyosin, CuZn super-oxide dismutase (SOD), triosephosphate isomerase, EP protein and small heat shock protein were identified. SOD and tropomyosin changes were verified by spectrophotometric measurements and western blotting, respectively. As some of the proteins identified are related to muscular damage and oxidative stress, other biomarkers associated with these processes that can be evaluated by automatic biochemical assays were measured including troponin, creatine kinase (CK), and aspartate aminotransferase (AST) for muscle damage, and SOD, trolox equivalent antioxidant capacity (TEAC) and esterase activity (EA) for oxidative stress. Significantly higher concentrations of troponin, CK, AST, and TEAC were observed in mussels after puncture, being also possible biomarkers of non-specific induced damage.
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Citation
Fish & Shellfish Immunology. Volume 75, April 2018, Pages 41-47
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