Publication: Comparison of MR images and histochemical localization of intra-arterially administered microglia
surrounding ß-amyloid deposits in the rat brain
Authors
Song, Y. ; Morikawa, S. ; Morita, M. ; Inubushi, T. ; Takada, T. ; Torii, R. ; Kitamura, Y. ; Taniguchi, T. ; Tooyama, I.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The therapeutic use of microglial cells has
recently received some attention for the treatment of
Alzheimer disease (AD), but few non-invasive
techniques exist for monitoring the cells after
administration. Here we present a magnetic resonance
imaging (MRI) technique for tracking microglia injected
intra-arterially in vivo. We micro-injected Aß42 into the
left hippocampus and saline into the right hippocampus
of rats. We then administered microglia, which were
labeled with enhanced green fluorescent protein (EGFP)
gene and Resovist, into the carotid artery. After
monitoring exogenously administered microglia using
MRI, we compared the MR images and the
histochemical localization of administered microglia.
MRI revealed clear signal changes attributable to
Resovist-containing microglia in Aß-injected areas.
Histochemistry demonstrated that EGFP-positive
microglia accumulated around Aß deposits and
internalized the peptide. This study demonstrates the
usefulness of MRI for non-invasive monitoring of
exogenous microglia, and suggests a promising future
for microglia/macrophages as therapeutic tools for AD.
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