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Phylogeny of cytokines: molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1b

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dc.contributor.authorScapigliati, G.
dc.contributor.authorBuonocore, F.
dc.contributor.authorBird, S.
dc.contributor.authorZou, J.
dc.contributor.authorFalasca, C.
dc.contributor.authorPrugnoli, D.
dc.contributor.authorSecombes, C. J.
dc.contributor.authorPelegrín Vivancos, Pablo
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.date.accessioned2024-01-24T17:50:11Z
dc.date.available2024-01-24T17:50:11Z
dc.date.issued2001
dc.description©2001. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Fish & Shellfish Immunology. To access the final edited and published work see https://doi.org/10.1006/fsim.2001.0347es
dc.description.abstractIn this paper the cloning of interleukin-1b (IL-1b) from the fish Dicentrarchus labrax (sea bass) is described. Using degenerate primers designed from known IL-1b sequences, a cDNA fragment was amplified by PCR and elongated by 3’ and 5’ RACE to give the full-length coding sequence for sea bass IL-1. The cDNA is 1292 bp, lacks a putative ICE cut site, and codes for a deduced peptide of 29.4 kDa with a pI of 5.1. Sequence analysis showed highest amino acid similarity with rainbow trout (62%), Xenopus (46%), and carp (45.5%) IL-1b sequences. Expression studies show that sea bass IL-1b can be upregulated by bacterial lipopolysaccharide both in vitro and in vivo in leucocytes from blood, head-kidney, spleen, gills and liver, whereas the IL-1b transcript was not detectable in thymus and gut-associated lymphoid tissue. Northern blot analysis with head-kidney leucocyte RNA showed a main LPS- upregulated band at 1.3 kb, and two minor bands at 0.9 and 3.0 kb, respectively. Phylogenetic comparisons with IL-1b from other vertebrates is presented.es
dc.formatapplication/pdfes
dc.format.extent33es
dc.identifier.citationFish & Shellfish Immunology, volumen 11, nº 8, año 2001, páginas 711-726.
dc.identifier.doi10.1006/fsim.2001.0347
dc.identifier.issn1050-4648
dc.identifier.urihttp://hdl.handle.net/10201/137724
dc.languageenges
dc.relationBBSRC y ERASMUSes
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S105046480190347X?via%3Dihubes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectInterleucina-1es
dc.subjectPeceses
dc.subjectLubinaes
dc.subjectCitocinases
dc.subjectEvolutiones
dc.subjectLeucocitoses
dc.subject.otherCDU::6 - Ciencias aplicadases
dc.titlePhylogeny of cytokines: molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1bes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication547c4c2c-d906-4e1d-ae9a-0cdb40eef6d1
relation.isAuthorOfPublication.latestForDiscovery547c4c2c-d906-4e1d-ae9a-0cdb40eef6d1
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