Publication: Phylogeny of cytokines: molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1b
Authors
Scapigliati, G. ; Buonocore, F. ; Bird, S. ; Zou, J. ; Falasca, C. ; Prugnoli, D. ; Secombes, C. J. ; Pelegrín Vivancos, Pablo
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DOI
10.1006/fsim.2001.0347
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info:eu-repo/semantics/article
Description
©2001. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted version of a Published Work that appeared in final form in Fish & Shellfish Immunology. To access the final edited and published work see https://doi.org/10.1006/fsim.2001.0347
Abstract
In this paper the cloning of interleukin-1b (IL-1b) from the fish Dicentrarchus labrax (sea bass) is described. Using degenerate primers designed from known IL-1b sequences, a cDNA
fragment was amplified by PCR and elongated by 3’ and 5’ RACE to give the full-length coding sequence for sea bass IL-1. The cDNA is 1292 bp, lacks a putative ICE cut site, and codes for a deduced peptide of 29.4 kDa with a pI of 5.1. Sequence analysis showed highest amino acid similarity with rainbow trout (62%), Xenopus (46%), and carp (45.5%) IL-1b sequences. Expression studies show that sea bass IL-1b can be upregulated by bacterial lipopolysaccharide both in vitro and in vivo in leucocytes from blood, head-kidney, spleen, gills and liver, whereas the IL-1b transcript was not detectable in thymus and gut-associated lymphoid tissue. Northern blot analysis with head-kidney leucocyte RNA showed a main LPS- upregulated band at 1.3 kb, and two minor bands at 0.9 and 3.0 kb, respectively. Phylogenetic comparisons with IL-1b from other vertebrates is presented.
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Citation
Fish & Shellfish Immunology, volumen 11, nº 8, año 2001, páginas 711-726.
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