Browsing by Subject "Curcumin"
Now showing 1 - 6 of 6
Results Per Page
Sort Options
- PublicationOpen AccessAccelerated Simple Preparation of Curcumin-Loaded Silk Fibroin/Hyaluronic Acid Hydrogels for Biomedical Applications(MDPI, 2023-01-18) Chaala, M.; Seeba, F. Z.; Fuster, M. G.; Moulefera, I.; Montalbán, M. G.; Carissimi, G.; Víllora, G.; Ingeniería Química; Facultad de QuímicaThe development of new biomaterials from natural fibres in the field of biomedicine have attracted great interest in recent years. One of the most studied fibres has been silk fibroin produced by the Bombyx mori worm, due to its excellent mechanical properties and its biodegradability and bioavailability. Among the different biomaterials that can be prepared from silk fibroin, hydrogels have attracted considerable attention due to their potential use in different fields, such as scaffolding, cell therapy and biomedical application. Hydrogels are essentially a three-dimensional network of flexible polymer chains that absorb considerable amounts of water and can be loaded with drugs and/or cells inside to be used in a wide variety of applications. Here we present a simple sonication process for the preparation of curcumin-hyaluronic acid-silk fibroin hydrogels. Different grades of hydrogels were prepared by controlling the relative amounts of their components. The hydrogels were physically and morphologically characterised by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and field emission scanning electron microscopy (FESEM) and their biological activity was tested in terms of cell viability in a fibroblast cell line.
- PublicationOpen AccessAnti-inflammatory effects of enemas containing an oily extract of curcumin in an experimental model of diversion colitis(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Jaoudat Kadri, Caled; Aires Pereira, José; Guilherme Campos, Fábio; Marques Ortega, Manoela; Benediti Bragion, Celene; Real Martinez, Carlos AugustoCurcumin has powerful anti-inflammatory and antioxidant effects and it has been used for treatment of distal ulcerative colitis. The therapeutic effects of curcumin have not yet been evaluated in diversion colitis. The aim of the present study was to evaluate the anti-inflammatory effects of curcumin on colonic mucosa devoid of a faecal stream. Thirty-six rats were subjected to a proximal colostomy and distal colonic fistulation. They were divided into two groups, which were sacrificed two or four weeks after the intervention. Each group was divided into three subgroups treated with the daily application of enemas containing saline or an oily extract of curcumin at 50 mg/kg/day or 200 mg/kg/day. Colitis was diagnosed by histological analysis. Inflammatory grades were assessed using a previously validated scoring system. The infiltration of neutrophils was evaluated based on the tissue expression of myeloperoxidase (MPO), as determined by immunohistochemistry, and a computer-assisted image analysis program. The Mann-Whitney test was used to compare inflammation grades and myeloperoxidase levels among groups, and ANOVA was used to verify the variance over time, with the level of significance set at 5% (p<0.05) for both tests. Enemas containing curcumin improved the inflammation of the mucosa without a faecal stream and reduced the tissue contents of MPO. MPO tissue levels did not vary with time or between the concentrations of curcumin used. Enemas with curcumin improved the inflammation of the colonic mucosa, reduced the inflammatory grade and decreased the tissue content of MPO in colon segments without a faecal stream.
- PublicationOpen AccessEficacia de la Cúrcuma Longa (Zingiberacea) vs. Clorhexidina al 0.2% contra bacterias periodontales : estudio in vitro(Universidad de Murcia, 2024-11-13) Carpio González, Ana María de las Mercedes; Camacho Alonso, Fabio; Corigliano, Massimo; Escuela Internacional de DoctoradoEn los últimos años, se ha despertado el interés por los agentes antimicrobianos naturales debido a la alta resistencia bacteriana. La Cúrcuma Longa una planta perenne que pertenece a la familia Zingiberaceae, ha generado el interés de la comunidad científica debido a sus propiedades antimicrobianas y antiinflamatorias. Objetivo: Determinar la eficacia antibacteriana de los extractos de Cúrcuma L. a diferentes concentraciones vs. la Clorhexidina al 0.2%, en cepas periodontales in vitro: (Porphyromonas gingivalis ATTC ® 33277, Tanneretta forsythia ATTC 43037, Treponema denticola ATTC ®35405, Peptostreptococcus micros ATTC ®33270 y Aggregatibacter actinomycetemcomitans ®ATTC 3338). Metodología: Se diseñó un estudio experimental de tipo exploratorio, analítico y según su temporalidad, de tipo longitudinal. Se prepararon diferentes tipos de extractos (etanólico, metanólico y acuoso) de Cúrcuma de la India y de la Cúrcuma cultivada en la República Dominicana, y se prepararon soluciones de Cúrcuma a diferentes concentraciones de cada una (100, 50, 25, 12.50, 6.25, 3.125, 1.562, 0.780, 0.390, 0.195 mg/ml). Se realizaron 5 repeticiones con cada grupo para una muestra total de 185 réplicas. Para evaluar la eficacia antibacteriana de la Cúrcuma y la Clorhexidina al 0.2%, se utilizó el método por difusión de discos y se modificó utilizando los medios de cultivos nutritivos y los requerimientos para cada cepa según el Global Bioresource Center (ATTC). Determinamos la eficacia de los extractos de Cúrcuma L. y de la Clorhexidina al 0.2% midiendo el halo de eficacia en mm. Resultados: Se observó que la Cúrcuma L. solamente tuvo eficacia frente a la Peptostreptococcus micros a concentración mínima de 50 mg/ml en los extractos (etanólico y metanólico) y a 100mg/ml en el (acuoso) con un promedio de halo de eficacia de 7mm, mientras que la Clorhexidina al 0.2 % mostró una mayor eficacia de todas las cepas analizadas con un promedio de halo de inhibición de 11.8 mm. Conclusión: Los extractos de los rizomas de la Cúrcuma L. presentaron un efecto antibacteriano frente a la Peptostreptococcus micros a una concentración mínima de 50mg/ml. La eficacia de la Clorhexidina en gel al 0.2% es superior a la de la Cúrcuma L. Se rechaza la hipótesis de igualdad entre ambos productos con un p valor = 0.00 < 0.05. La Clorhexidina sigue siendo el agente antiséptico tópico de uso en la terapia periodontal con una mayor eficacia antibacteriana.
- PublicationOpen AccessIn vitro and in vivo efficacy evaluation of new self-assembling curcumin loaded nanohyaluronan-glycerosomes on wound restoring in health and diabetic rats(Elsevier, ) Gómez García, Francisco José; Ramírez Andreo, Antonio; Manconi, María; Manca, María Letizia; Matricardi, Pietro; Di Meo, Chiara; Fernàndez-Busquets, Xavier; Martínez Díaz, Francisco; Salmerón Martínez, Diego; López Jornet, Pía; Ciencias SociosanitariasIn this study the wound healing efficacy of new self-assembling curcumin loaded nanohyaluronan-glycerosomes has been tested in healthy and diabetic rats. Curcumin was loaded in nanohyaluronan-liposomes and -glycerosomes, special vesicles obtained mixing curcumin, hyaluronan nanohydrogel and soy lecithin. Curcumin loaded liposomes and glycerosomes were also prepared, characterized and tested as references. The physico-chemical (size and size distribution, surface charge and morphology), and technological (entrapment efficiency, stability over time and skin delivery) characteristics and biological performances (safety, ability to protect in vitro cells from oxidative damage) of prepared formulations were measured. Finally, they were daily applied to skin wounds of healthy or diabetic rats. The animal skin was excided and analysed at days 3, 7 and 14. The macroscopic observation underlined the higher ability of curcumin loaded nanohyaluronan-glycerosomes to improve wound healing in diabetic rats, already on day 3 up to day 14 (p < 0.05). Histopathological analysis confirmed an accelerated re-epithelization in healthy rats, while increased angiogenesis in diabetic ones. The superior therapeutic efficacy of curcumin loaded nanohyaluronan-glycerosomes compared to other formulations can be related to their enhanced ability to deliver higher curcumin concentrations at the wound site due to a synergistic effect of glycerol, hyaluronan nanohydrogel and lecithin.
- PublicationOpen AccessInhibitory effect of Curcumin on a cervical cancer cell line via the RAS/RAF signaling pathway(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Özdemır, İlhan; Zaman, Fuat; Baş, Dilek Doğan; Sari, Umut; Öztürk, Şamil; Tunce, Mehmet CudiObjective. Cervical cancer has a very important place in female infertility and ranks fourth among cancers affecting women. Curcumin (CUR) is closely associated with the expression and activity of various regulatory proteins. It is also known that curcumin has preventive and therapeutic effects on various types of cancer. In this study, the anticancer activities of curcumin were demonstrated in the human cervical cancer cell line (HeLa). Methods. qRT-PCR and western blot analyses were used to evaluate mRNA and protein expression of curcumin in HeLa and immortalized human skin keratinocyte cell lines (HaCaT) (proliferation and apoptosis regulatory markers of the RAS/RAF signaling pathway). MTT analysis was performed, showing HeLa and HaCaT cell proliferation depending on the dose and duration of curcumin and doxorubicin. A wound scratch healing assay was applied to examine cell migration and invasion of HeLa after curcumin application. To determine the role of curcumin and doxorubicin in the apoptosis of HeLa cells, the mRNA levels of caspase-3 were examined by qRT-PCR. The results were analyzed with a one-way ANOVA SPSS 20.0 program. Results. CUR (IC50: 242.8 μM) and DOX (IC50: 92.1 μM) were determined to have the ability to inhibit the proliferation of HeLa cells and induce apoptosis over a 72-hour period and dose-dependently. Moreover, the results revealed that the mRNA and protein expression levels of RAF and RAS in HeLa cells were downregulated by CUR and DOX. Conclusions. The findings show that an alternative treatment method for cervical cancer can be developed with the application of CUR and DOX. Alternative methods for cervical cancer treatment may be developed using different methods in future studies.
- PublicationOpen AccessThe potential protective effects of curcumin on the diabetic ovary: Experimental and molecular approaches(Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Tufekci, Kıymet Kübra; Altun, Gamze; Kipanyula, Maulilio John; Kaplan, Süleyman; Departamento de Biologia Celular e HistiologiaDiabetes mellitus (DM) causes numerous systemic diseases in animals and humans. This may also lead to reproductive problems among individuals of reproductive age. Detrimental effects such as apoptosis in ovarian granulosa cells, degradation of communica-tion proteins, decreased oocyte quality, delayed meiotic maturation, and atrophy are among the increasing evidence that chronic hyperglycemia causes reproductive problems. Numerous studies have reported that the antidiabetic properties of the antioxidant curcumin may be due to its inhibition of oxidative stress, inflammation, and insulin resistance. There are also data indicating that curcumin reduces the risk of DM and its associated symptoms. This review discusses the protective or curative properties of curcumin in the treatment of DM-related problems in the ovary and seeks to elucidate potential underlying mechanisms. While the use of curcumin as a supportive/therapeutic agent has been introduced for the reduction of reproductive problems that may be caused by uncontrolled DM, more studies on this subject are needed.