Publication: Feeding entrainment of food-anticipatory activity and per1 expression in the brain and liver of zebrafish under different lighting and feeding conditions
Authors
López Olmeda, José Fernando ; Tartaglione, Erika ; Iglesia, Horacio de la ; Sánchez Vázquez, Francisco Javier
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Publisher
Taylor and Francis Group [Commercial Publisher]
Taylor and Francis [Imprint]
Taylor and Francis [Imprint]
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Description
©<2010>. This manuscript version is made available under the CC-BY license http://creativecommons.org/licenses/ccby/4.0/
This document is the Acepted, version of a Published Work that appeared in final form in [Chronobiology International].To access the final edited and published work see [https://doi.org/10.3109/07420528.2010.501926]
Abstract
Food provided on a periodic basis can act as a potent synchronizer, being a stronger
zeitgeber than light for peripheral oscillators in mammals. In fish, however, little is
known about the influence of feeding time on the circadian pacemaker and the
relationship between central and peripheral oscillators. The aim of this research was
to investigate the influence of mealtime on the activity rhythms, and on central
(brain) and peripheral (liver) oscillators in zebrafish. The authors tested different
feeding times under a light-dark (LD) cycle and the endogenous origin of food-
anticipatory activity (FAA) by feeding zebrafish at a fixed time under constant bright-
light conditions (LL). The authors then measured locomotor activity and the
expression of the clock gene per1 in animals under a LD cycle and fed at random
times during the light phase, with restricted feeding at the mid-light phase (ML) or
with restricted feeding during the mid-dark phase (MD). Finally, the authors
measured locomotor activity and per1 expression in fish maintained under LL under
either random feeding or scheduled feeding. Zebrafish displayed FAA in all the
groups fed at a fixed time but not when feeding was randomly scheduled. Under LL,
fish entrainment persisted, and when released under fasting conditions FAA free-run
with a circa-24-h period. The expression of per1 in the brain of fish under LD
showed a daily rhythm with the acrophase (peak time) at the end of the dark phase
regardless of feeding schedule. This brain rhythm disappeared in LL fish under both
random feeding and scheduled feeding. Feeding at MD advanced the phase of per1
in the liver by 7 h compared with the ML-fed group phase (23:54 versus 07:23 h,
respectively). In addition, under LL scheduled feeding entrained the rhythms of per1
expression in the liver. This study reveals for the first time that scheduled feeding
entrains peripheral oscillators in a fish species, zebrafish, which is a powerful model
widely used for molecular genetics and for the study of basic clock mechanisms of the
vertebrate circadian system.
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