DigitalUM :: Browsing by browse.metadata.contributordepartment "Fisiología"

Browsing by browse.metadata.contributordepartment "Fisiología"

Now showing 1 - 20 of 231

Open Access
A blind circadian clock in cavefish reveals that opsins mediate peripheral clock photoreception
(Public Library of Science, 2011) Cavallari, Nicola; Frigato, Elena; Vallone, Daniela; Froehlich, Nadine; López Olmeda, José Fernando; Foa, Augusto; Berti, Roberto; Sánchez Vázquez, Francisco Javier; Bertolucci, Cristiano; Foulkes, Nicholas Simon; Fisiología
The circadian clock is synchronized with the day-night cycle primarily by light. Fish represent fascinating models for deciphering the light input pathway to the vertebrate clock since fish cell clocks are regulated by direct light exposure. Here we have performed a comparative, functional analysis of the circadian clock involving the zebrafish that is normally exposed to the day-night cycle and a cavefish species that has evolved in perpetual darkness. Our results reveal that the cavefish retains a food-entrainable clock that oscillates with an infradian period. Importantly, however, this clock is not regulated by light. This comparative study pinpoints the two extra-retinal photoreceptors Melanopsin (Opn4m2) and TMT-opsin as essential upstream elements of the peripheral clock light input pathway.
Open Access
A metabolic signature predicts biological age in mice
(Wiley Open Access, 2013-02) Tomas-Loba, Antonia; Bernardes de Jesus, Bruno; Mato, Jose M; Blasco, Maria Antonia; Fisiología
Our understanding of the mechanisms by which aging is produced is still very limited. Here, we have determined the sera metabolite profile of 117 wild-type mice of different genetic backgrounds ranging from 8 to 129 weeks of age. This has allowed us to define a robust metabolomic signature and a derived metabolomic score that reliably/accurately predicts the age of wild-type mice. In the case of telomerase-deficient mice, which have a shortened lifespan, their metabolomic score predicts older ages than expected. Conversely, in the case of mice that overexpress telomerase, their metabolic score corresponded to younger ages than expected. Importantly, telomerase reactivation late in life by using a TERT-based gene therapy recently described by us significantly reverted the metabolic profile of old mice to that of younger mice, further confirming an anti-aging role for telomerase. Thus, the metabolomic signature associated with natural mouse aging accurately predicts aging produced by telomere shortening, suggesting that natural mouse aging is in part produced by presence of short telomeres. These results indicate that the metabolomic signature is associated with the biological age rather than with the chronological age. This constitutes one of the first aging-associated metabolomic signatures in a mammalian organism.
Embargo
A velocity-based approach to variable resistance training in the bench press: repetitions to failure and level of effort
(Lippincott, Williams & Wilkins, 2026-03) Ocaña-García, Álvaro; Herrero-Atiénzar, José Manuel; López Gullón, José María; Bonacasa Fernández, Bárbara; Bayonas-Ruiz, Adrián; Fisiología
This study analyzed the use of elastic bands in the bench press exercise within a velocity-based training approach to explore its application in variable resistance training (VRT) in regard to the level of effort methodology. Therefore, we aimed to (a) investigate the intersubject and intrasubject variability in the number of repetitions to failure (nRM) across 3 one-repetition maximum percentages (%1RM) and 2 load configurations using elastic bands: 25% (B25) and 50% (B50) of the load from bands; (b) examine the relationship between the number of repetitions completed and velocity loss reached; (c) assess the mean propulsive velocity associated to each %1RM and elastic bands contribution; and (d) study the influence of the subject’s strength level on these parameters. After determining the individual 1RM, 26 subjects with low (n 5 9), medium (n 5 9), and high (n 5 8) relative strength levels completed 2 rounds of nRM tests against their 60, 70, and 80% 1RM with B25 and B50 configurations in the bench press. Number of repetitions to failure increased with band contribution (B50 . B25, p , 0.01) and showed moderate intersubject variability (coefficients of variation 5 16%) and very low intrasubject (SEM # 1.4 repetitions) variability for all the strength levels, % 1RM, and band contributions analyzed. A very high correlation (R 2 5 0.910–0.935) between the number of repetitions completed and the percentage of velocity loss reached (from 5 to 65%) was found. These findings support the use of the level of effort as a reliable and practical tool for programming VRT.
Open Access
Academic climate and psychopathological symptomatology in Spanish medical students
(BMC, 2023-11-07) Garcia Estañ López, Joaquín María; Esquerda, Montse; Ruiz Rosales, Albert; García Abajo, J. Miguel; Millán, Jesús; Fisiología
Introduction: Medical Education studies suggest that medical students experience depression, anxiety and psy‑chopathological symptomatology in a proportion higher than in the rest of the population. In the present study, we aimed to conduct a nationwide analysis to describe student’s perceptions of Educational Climate in Spanish medical schools, and its relationship with psychopathological symptomatology. Methods . The study was carried out in 2022 in all 44 medical schools in Spain, and analyses the academic climate, and psychopathological symptomatology among medical students (n=4374). To measure these variables, we used the Dundee Ready Education Environment Measure (DREEM) for academic climate, and the SA-45 (Symptom Assess‑ ment-45 Questionnaire was used to assess psychopathological symptomatology. Results: The mean DREEM global score was low, 95.8 (SD 22.6). Worse perception of the academic climate has been found in females (t -2.21, p 0.027), in students of the clinical academic years (t 16.9, p<0.001), and public medical schools ( t 15.6, p<0.001). The SA45 general index score was high (p90) in 25.6% of participants. In respect of gender, female students presented higher levels of SA45 general index score, depression, interpersonal sensitivity, somatization, anxiety, obsession-com‑ pulsion, and phobic anxiety symptoms. Higher DREEM global and subscale scores corresponded to a higher SA-45 global index score and higher SA-45 sub‑ scale scores. Conclusions: Our study suggests a correlation between a poor perception of academic climate, increased depres‑ sion, anxiety, and other psychopathological symptoms, with a pattern that varies between diferent faculties. The perception of academic climate varied between medical schools, as did the psychopathological symptoms scores. Our fnding suggests the prevalence of these variables in medical students is, at least in part, attributable to factors directly related to the learning atmosphere.
Restricted
Activity-rest circadian pattern and academic achievement, executive function, and intelligence in children with obesity
(Wiley, 2020-10-23) Migueles, Jairo H.; Martínez Nicolás, Antonio; Cadenas Sánchez, Cristina; Esteban Cornejo, Irene; Muntaner Mas, Adrià; Mora González, José; Rodríguez Ayllón, María; Madrid, Juan Antonio; Rol de Lama, María de los Ángeles; Hillman, Charles H.; Catena, Andrés; Ortega, Francisco B.; Fisiología
This study aimed to analyze the associations of activity-rest pattern indicators with academic achievement, executive function, and intelligence and to explore whether these associations are mediated by the total gray matter volume among children with overweight/obesity. Ninety-five children (10 ± 1 year, 37 girls) with overweight/obesity (based on the World Obesity Federation body mass index cutoff points) were included in this cross-sectional study. Hip- and wrist-worn ActiGraph GT3X + accelerometers were used to assess the activity-rest pattern. Interdaily stability (IS), intradaily variability (IV), the mean value of the lowest 5 hours (L5), and the mean value of the maximum 10 hours (M10) of activity and their respective timing (TL5, TM10) were used as indicators of the activity-rest pattern throughout the day. Chronotype and social jetlag were used as indicators of circadian preference. Academic achievement, executive function, and intelligence were assessed with standardized tests. Gray matter volume was acquired by magnetic resonance imaging (MRI). IS was positively associated with executive function (β = 0.244, P = .014). IV was negatively associated with mathematics and academic applications (β: −0.211 to −0.238, P’s ≤ .026). Later TM10 in the day was related to lower writing, academic skills, and intelligence (β: −0.229 to −0.271, P’s ≤ .025). None of the associations found were mediated by gray matter volume. A non-fragmented and stable activity-rest pattern and earlier physical activity in the day were associated with better academic achievement, executive function, and intelligence in children with overweight/obesity. Further studies are required to corroborate or contrast our findings.
Open Access
Adiponectin accounts for gender differences in hepatocellular carcinoma incidence
(Rockefeller University Press, 2019-05-06) Manieri, Elisa; Herrera-Melle, Leticia; Mora, Alfonso; Tomas-Loba, Antonia; Leiva-Vega, Luis; Fernandez, Delia I; Rodriguez, Elena; Moran, Laura; Hernández-Cosido, Laura; Torres, Jorge L; Seane, Luisa M; Cubero, Francisco Javier; Marcos, Migue; Sabio, Guadalupe; Fisiología
Open Access
La administración a cerdas de cloprostenol 24 horas después del parto reduce el intervalo destete-cubrición y mejora la tasa de partos en el ciclo siguiente
Crespo, S.; Gadea Mateos, Joaquín; Fisiología
Restricted
Age- and Sodium-Sensitive Hypertension and Sex-Dependent Renal Changes in Rats With a Reduced Nephron Number
(American Heart Association, 2008-02-07) Salazar, Francisco; Reverte, Virginia; Saez, Fara; Lorai, Analia; Llinas, Maria Teresa; Salazar, F. Javier; Fisiología
We have demonstrated that the reduction of angiotensin II effects during the nephrogenic period reduces the nephron number and induces the development of hypertension. The hypotheses examined are that this reduction of angiotensin effects leads to the development of an age-dependent sodium sensitive hypertension and that the hypertension is angiotensin II dependent. Newborn rats were treated with an angiotensin II type 1 receptor antagonist during the first 2 weeks of age. At 3 to 4 and 11 to 12 months of age, changes in systolic blood pressure, proteinuria, and renal function in response to a prolonged high sodium intake were examined. The basal blood pressure response to the administration of the angiotensin II receptor antagonist was also evaluated at both ages. Basal blood pressure was similarly elevated (P<0.05) in male and female treated rats, and the increment was age dependent. High sodium intake only elicited a blood pressure elevation (136±1 to 154±3 mm Hg; P<0.05) and a decrease in glomerular filtration rate (28%; P<0.05) at 11 to 12 months in treated rats. Blockade of angiotensin II receptors during renal development induced an increase (P<0.05) in proteinuria that was age and sex dependent, but high sodium intake only induced an elevation in proteinuria in the younger rats (50%; P<0.05). Hypertension was maintained by angiotensin II at both ages because blood pressure decreased to normal levels after treatment with an angiotensin II type 1 receptor antagonist. This study shows that the reduction of angiotensin II effects during the nephrogenic period modifies renal function and induces the development of an angiotensin II–dependent hypertension that becomes sodium sensitive during aging.
Restricted
Age-related changes in fatty acid profile and locomotor activity rhythms in Nothobranchius korthausae
(Elsevier, 2011-08-28) Lucas-Sanchez, A; Almaida Pagán, Pedro Francisco; Madrid, Juan A; Costa, J. de; Mendiola, P; Fisiología
The life cycle of Nothobranchius korthausae, a Cyprinodontiformes fish, was studied in our laboratory to characterise the ageing process. Some morphological changes, such as spine curvature, skin colour, and fin and eye appearance are described. Growth and survival curves reflected a fast life cycle with rapid initial growth until 4 weeks of age, after which the fish grew more slowly before reaching their final size in week 40. Senescence onset was established at week 48 with a decrease in spawn size and viability and a general decline in the animal's appearance (weight and colouration losses, caudal fin degradation, and cataractogenesis). The fatty acid composition changed with age,with high unsaturation in the adult stage as reflected by a high peroxidation index, a condition that is associated with high susceptibility to oxidative damage if elevated reactive oxygen species (ROS) production occurs. Senescent fish had an increase in monounsaturated fatty acid proportions and a lower peroxidation index (226.5±19.7 in adults versus 120.2±19.1 in senescent fish, Pb0.05). The circadian system, as reflected by locomotor activity rhythms, showed noticeable changes with age. Twenty-four-week-old fish (adults) had a robust diurnal rhythm that showed a decrease in total activity, an increase in rhythmfragmentation, and a fall in amplitude and regularity with age. Changes were clearly reflected in the Circadian Function Index variations (0.56, 0.47 and 0.25 at 24, 48 and 72 weeks of age, respectively). In conclusion, N. korthausae appears to be a species with appropriate characteristics for ageing studies because it manifests clear signs of progressive ageing. Comparing species of Nothobranchius genus with different lifespans may be useful for increasing our understanding of the ageing process.
Open Access
Age-related changes in mitochondrial membrane composition of Nothobranchius furzeri. Comparison with a longer-living Nothobranchius species
(Springer Nature, 2019-02-15) Almaida Pagán, Pedro Francisco; Ortega-Sabater C.; Lucas-Sanchez A.; Gonzalez-Silvera D.; Martinez-Nicolas A.; Rol de Lama, María de los Ángeles; Mendiola P.; de Costa J.; Fisiología
Membrane compositions, particularly of mitochondria, could be critical factors in the mechanisms of growth and aging, especially during phases of high oxidative stress that result in molecular damage. Changes affecting lipid class or fatty acid (FA) compositions could affect phospholipid (PL) properties and alter mitochondrial function. In the present study, mitochondrial membrane PL compositions were analysed throughout the life-cycle of Nothobranchius furzeri, a species with explosive growth and one of the shortest-lived vertebrates. Mitochondrial PLs showed several changes with age. Proportions of total PLs and PC were reduced while an increase in PS, CL and PE was observed, mainly between the 2.5 and 5 months of fish age, the time during which animals doubled their weight. FA compositions of individual PLs in mitochondria were also significantly affected with age suggesting the existence of increasing damage to mitochondrial lipids during the life- cycle of N. furzeri that could be one of the main contributors to degraded mitochondrial function associated with aging. The peroxidation index values from N. furzeri mitochondrial PLs were significantly lower than those reported in N. rachovii, a species with a 2-fold longer life span than N. furzeri, which seems to contradict the membrane pacemaker theory of animal metabolism
Restricted
Age-related changes in mitochondrial membrane composition of nothobranchius rachovii
(Oxford University Press, 2013-05-17) Lucas-Sánchez, Alejandro; Almaida Pagán, Pedro Francisco; Tocher, Douglas R.; Mendiola, Pilar; Costa, Jorge de; Fisiología
Mitochondrial membrane composition may be a critical factor in the mechanisms of the aging process by influencing the propagation of reactions involved in mitochondrial function during periods of high stress. Changes affecting either lipid class or fatty acid compositions could affect phospholipid properties and alter mitochondrial function and cell viability In the present study, mitochondrial membrane phospholipid compositions were analyzed throughout the life cycle of Nothobranchius rachovii. Mitochondrial phospholipids showed several changes with age. Proportions of cardiolipin decreased and those of sphingomyelin increased between 11- and 14-month-old fish. Fatty acid compositions of individual phospholipids in mitochondria were also significantly affected with age. These data suggest increasing damage to mitochondrial lipids during the life cycle of N. rachovii that could be one of the main factors related with and contributing to degraded mitochondrial function associated with the aging process.
Restricted
Age-related changes in mitochondrial membrane composition of rainbow trout (Oncorhynchus mykiss) heart and brain
(Elsevier, 2012-05-22) Almaida Pagán, Pedro Francisco; Costa, J. de; Mendiola, P; Tocher, D R; Fisiología; Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, Scotland, United Kingdom; Department of Physiology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain
Membrane composition, particularly of mitochondria, could be a critical factor by determining the propaga-tion of reactions involved in mitochondrial function during periods of high oxidative stress such as rapid growth and aging. Considering that phospholipids not only contribute to the structural and physical properties of biological membranes, but also participate actively in cell signaling and apoptosis, changes affecting either class or fatty acid compositions could affect phospholipid properties and, thus, alter mitochondrial function and cell viability. In the present study, heart and brain mitochondrial membrane phospholipid compositions were analyzed in rainbow trout during the four first years of life, a period characterized by rapid growth and a sustained high metabolic rate. Specifically, farmed fish of three ages (1-, 2- and 4-years) were studied, and phospholipid class compositions of heart and brain mitochondria, and fatty acid compositions of individual phospholipid classes were determined. Rainbow trout heart and brain mitochon- dria showed different phospholipid compositions (class and fatty acid), likely related to tissue-specific functions. Furthermore, changes in phospholipid class and fatty acid compositions with age were also tissue-dependent. Heart mitochondria had lower proportions of cardiolipin (CL), phosphatidylserine (PS) and phosphatidylinositol, and higher levels of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) with age. Heart mitochondrial membranes became more unsaturated with age, with a significative increase of peroxidation index in CL, PS and sphingomyelin (SM). Therefore, heart mitochondria became more susceptible to oxidative damage with age. In contrast, brain mitochondrial PC and PS content decreased in 4-year-old animals while there was an increase in the proportion of SM. The three main phospholipid classes in brain (PC, PE and PS) showed decreased n−3 polyunsaturated fatty acids, docosahexaenoic acid and peroxidation index, which indicate a different response of brain mitochondrial lipids to rapid growth and maturation.
Open Access
Ageing of the circadian system. From monitoring to chronoenhancement.
(Spanish Society of Anti-Ageing Medicine and Longevity (SEMAL), 2018-10) Martinez-Nicolas, A; Almaida Pagán, Pedro Francisco; Martinez-Madrid, M J; Argüelles, R; Ortega-Sabater, C; Fernandez-Ortiz, M; Costa, J. de; Madrid, J A; Rol, M A; Fisiología; Chronobiology Lab, Department of Physiology, College of Biology, University of Murcia, Mare Nostrum Campus. IUIE, IMIB-Arrixaca, Spain.; Ciber Fragilidad y Envejecimiento Saludable (CIBERFES), Madrid, Spain.; Department of Physiology, College of Medicine, University of Granada, Granada, Spain
The circadian system (CS) organizes the temporal order of all living beings. Its general structure is very similar among species and consists of receptors of the temporal information (inputs), a central pacemaker along with several peripheral clocks that depend on it (machinery), and a set of overt rhythms driven by the central clock (outputs). The CS ages like any other structure of the organism, this process being characterized by a poorer reception of the temporal information, a general impairment of the central pacemaker and a phase advance, fragmentation and dampening of the overt rhythms. In order to assess the functioning of the CS, some overt rhythms have been selected as markers since they mirror the activity of the central pacemaker. Some of the most used marker rhythms are those of melatonin and cortisol secretion, rest-activity and sleep-wake patterns, and core body and distal skin temperature. Nevertheless, these rhythms can be masked by external variables and thus, to simultaneously record several marker rhythms is recommended. As the CS ages, the ability of an organism to adjust the internal temporal order of physiological, biochemical and behavioural circadian rhythms to the environmental cycles is compromised and chronodisruption can appear, which is related with several diseases. Fortunately, there are some strategies that one person can follow in order to enhance the functioning of the CS: to increase the contrast between day and night (i.e. to exposure to bright days and dark nights), to have melatonin (if needed), to do regular exercise, to improve sleep and meal schedules or to increase social contacts.
Open Access
Alteraciones de la función plaquetaria en la cirrosis hepática
(2015) Romecín, P.; Ortiz Ruiz, María Clara; Iyú Espinosa, David; García-Estañ López, Joaquín; Marín Atucha, Noemí; Fisiología
Open Access
Ambulatory circadian monitoring in sleep disordered breathing patients and CPAP treatment
(Nature Research, 2021-07-19) Martínez Nicolás, Antonio; Guaita, Marc; Santamaría, Joan; Montserrat, Josep M.; Madrid, Juan Antonio; Rol de Lama, María de los Ángles; Fisiología
Our aim was to evaluate the circadian rhythm of motor activity, body position and integrated variable TAP (composed by wrist Temperature, motor Activity and body Position) in Sleep Disordered Breathing (SDB), its relation to SDB severity and the effect of continuous positive airway pressure (CPAP) on these circadian rhythms. To do this, we monitored motor activity and body position rhythms of 78 SDB patients (53.3 ± 1.2 years old, 26.9% women) and 32 healthy subjects (51.4 ± 3.2 years old, 43.8% women) for 1 week. On the last day of that week, SDB patients underwent a polysomnography followed by a Maintenance of Wakefulness Test, Multiple Sleep Latency Test and Sustained Attention to Response Task protocol. A subgroup of 18 moderate to severe SDB patients was treated with CPAP and monitored again after 3 months under treatment. A non-parametrical analysis was performed to characterize the circadian patterns to assess differences between groups and associations between sleep and circadian parameters. Circadian variables were altered in SDB, exhibiting a direct relationship to SDB severity. The motor activity pattern showed a clear improvement with CPAP treatment. Thus, circadian ambulatory monitoring, including the integrated variable TAP, could be used to evaluate the circadian alterations caused by SDB and activity pattern to monitor the effect of CPAP treatment.
Open Access
Aminopeptidases in Cardiovascular and Renal Function. Role as Predictive Renal Injury Biomarkers
(MDPI, 2020-08-05) Vargas, Félix; Wangesteen, Rosemary; Rodríguez-Gómez, Isabel; García-Estañ, Joaquín; Fisiología
Aminopeptidases (APs) are metalloenzymes that hydrolyze peptides and polypeptides by scission of the N-terminus amino acid and that also participate in the intracellular final digestion of proteins. APs play an important role in protein maturation, signal transduction, and cell-cycle control, among other processes. These enzymes are especially relevant in the control of cardiovascular and renal functions. APs participate in the regulation of the systemic and local renin–angiotensin system and also modulate the activity of neuropeptides, kinins, immunomodulatory peptides, and cytokines, even contributing to cholesterol uptake and angiogenesis. This review focuses on the role of four key APs, aspartyl-, alanyl-, glutamyl-, and leucyl-cystinyl-aminopeptidases, in the control of blood pressure (BP) and renal function and on their association with different cardiovascular and renal diseases. In this context, the effects of AP inhibitors are analyzed as therapeutic tools for BP control and renal diseases. Their role as urinary biomarkers of renal injury is also explored. The enzymatic activities of urinary APs, which act as hydrolyzing peptides on the luminal surface of the renal tubule, have emerged as early predictive renal injury biomarkers in both acute and chronic renal nephropathies, including those induced by nephrotoxic agents, obesity, hypertension, or diabetes. Hence, the analysis of urinary AP appears to be a promising diagnostic and prognostic approach to renal disease in both research and clinical settings.
Open Access
An approach to the factors related to sperm capacitation process.
(2015-10-02) López Úbeda, Rebeca; Matas Parra, Carmen; Fisiología
Open Access
Analysis of the coding sequences of clownfish reveals molecular convergence in the evolution of lifespan
(Springer Nature, 2019-04-11) Sahm, Arne; Almaida Pagán, Pedro Francisco; Bens, Martin; Mutalipasi, Mirko; Lucas-Sánchez, Alejandro; Costa Ruiz, Jorge de; Görlach, Matthias; Cellerino, Alessandro; Fisiología
Standard evolutionary theories of aging postulate that reduced extrinsic mortality leads to evolution of longevity. Clownfishes of the genus Amphiprion live in a symbiotic relationship with sea anemones that provide protection from predators. We performed a survey and identified at least two species with a lifespan of over 20 years. Given their small size and ease of captive reproduction, clownfish lend themselves as experimental models of exceptional longevity. To identify genetic correlates of exceptional longevity, we sequenced the transcriptomes of Amphiprion percula and A. clarkii and performed a scan for positively-selected genes (PSGs).
Open Access
Assessing Chronotypes by Ambulatory Circadian Monitoring
(Frontiers Media, 2019-11-20) Martinez-Nicolas, A; Martinez-Madrid, M J; Almaida Pagán, Pedro Francisco; Madrid, Juan A; Rol, M A; Bonmatí Carrión, María de los Ángeles; Fisiología; Chronobiology Lab, Department of Physiology, College of Biology, University of Murcia, Mare Nostrum Campus, IUIE, IMIB-Arrixaca, Murcia, Spain; Ciber Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III, Madrid, Spain
In order to develop objective indexes for chronotype identification by means of direct measurement of circadian rhythms, 159 undergraduate students were recruited as volunteers and instructed to wear ambulatory circadian monitoring (ACM) sensors that continuously gathered information on the individual’s environmental light and temperature exposure, wrist temperature, body position, activity, and the integrated TAP (temperature, activity, and position) variable for 7 consecutive days under regular freeliving conditions. Among all the proposed indexes, the night phase marker (NPM) of the TAP variable was the best suited to discriminate among chronotypes, due to its relationship with the Munich ChronoType Questionnaire (b = 0.531; p < 0.001). The NPM of TAP allowed subjects to be classified as early- (E-type, 20%), neither- (N-type, 60%), and late-types (L-type, 20%), each of which had its own characteristics. In terms of light exposure, while all subjects had short exposure times to bright light (>100 lux), with a daily average of 93.84 5.72 min, the earlier chronotypes were exposed to brighter days and darker nights compared to the later chronotypes. Furthermore, the earlier chronotypes were associated with higher stability and day–night contrast, along with an earlier phase, which could be the cause or consequence of the light exposure habits. Overall, these data support the use of ACM for chronotype identification and for evaluation under free living conditions, using objective markers.
Open Access
Assessing feeding history and health status through analysis of fatty acids and fat content in golden mullet Liza aurata
(2016) Gonzalez-Silvera, D.; Martinez-Rubio, L.; Abad Mateo, M.E.; Rabadan-Ros, R.; Lopez-Jimenez, J.A.; Martinez Lopez, F.J.; Fisiología
The aim of this study was to check the suitability of using fatty acids of vegetable origin as biomarkers of aquafeed consumption in ﬁsh that aggregate around off-shore ﬁsh farms, analysing their different accumulation patterns and their persistence in different tissues in juveniles of Liza aurata (Risso, 1810). Their natural diet was replaced by a commercial feed, followed by a return to the natural diet (wash-out period). The fatty acid proﬁles of ﬂesh, liver, and brain were modiﬁed after 2 months of commercial feed consumption, while 2 months of the wash- out period were not sufﬁcient to return to original values, the brain being particularly resilient in this respect. Histological examination of the liver showed no alterations of the lipid droplet distribution or fat content. The combined use of ﬂesh and brain for fatty acid analysis can be recommended for tracking aquaculture waste intake in the form of lost pellets by wild ﬁsh.