Histology and histopathology Vol. 3, nº 3 (1988)

Ir a Estadísticas

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http://hdl.handle.net/10201/177669

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Eosin-related fluorescence of acidophil pituitary cells
(Murcia : F. Hernández, 1988) Friedman, H.; Friedman, I.V.C.; Mello, C.V.
The examination of haematoxylin and eosin stained sections of normal and neoplastic pituitary glands under ultraviolet light illumination discloses fluorescence of acidophil cells. The distinction between prolactin and growth hormone-producing cells is not possible. Such fluorescence depends on previous eosin staining.
Open Access
An ultrastructural stereologic study of aldosterone secreting adrenal adenomas and of adjacent zona glomerulosa
(Murcia : F. Hernández, 1988) Belloni, A. S.; Mantero, Franco; Rocco, Stefano; Gottardo, Giuseppe; Mazzocchi, G.; Nussdorfer, G.G.
The ultrastructure of four aldosterone secretine adenomas and of the adiacent zona glomerzosa has been described by "the use of stereological techniques. Adenomatous cells (about 2800 pm' in volume) invariably displayed a striking abundance of lipid droplets. which occupied about 30% of the cytoplasm. Mitochondria prevalently contained tubulo-lamellar or lamellar cristae, hut some cells exhibited organelles with vesicular cristae. Smooth endoplasmic reticulurn (SER) was not very abundant. Small lipofuscin-pigment granules were frequently seen and in a few cells they were exceedingly numerous. Zona glomerulosa cells were smaller (about 950 pm3 in \~olume) and possessed mitochondria with typical tubulo-lamellar cristae, a plentiful SER and few lipid droplets. They showed the ultrastructural features of elements actively engaged in steroid synthesis. The possible origin of aldosteronoma cells from the zona glomerulosa is discussed.
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Cytotoxic effects of 3-methylindole on alveolar epithelial cells and macrophages: with special reference to microtubular and filamentous assemblies in alveolar type I cells of bovine lung
(Murcia : F. Hernández, 1988) Atwal, Onkar S.; Minhas, Kanwal J.; Perry, Michael S.
The alveolar type I cell is a major permeability barrier between the pulmonary interstitium and alveolar spaces and its thin cytoplasmic processes are greatly susceptible to injury. These cells are often observed to undergo progressive vesiculation, vacuolization and desquamation during 3-methylindole (3MI)-induced acute pulmonary edema after oral administration in goats and cattle. The present study describes proliferation of SER and the presence of polymerized tubulin in the form of microtubules arranged in large bundles shown at ultrastructural level as well as with immunofluorescence staining for tubulin in alveolar type I cells 72 hours after 3MI treatment. Such changes were not seen in pulmonary endothelial cells, alveolar type I1 cells, alveolar macrophages and neutrophils. The possible role of microtubules in alveolar type I cell as a mechanistic support to resist disruption against the forces of interstitial and alveolar edema is compared with alveolar type I1 cells, alveolar macrophages and neutrophils. The latter cells undergo dynamic movements in response to inflammatory stimuli and therefore did not show microtubules in their cytoplasm
Open Access
Effects of isoproterenol on the fine structure of the hamster parathyroid gland
(Murcia : F. Hernández, 1988) Shizuko Shoumura; Yuji Iwasaki; Noriko Ishizaki; Shoichi Emura; Tomo Yamahira; Masaki Ito; Hideo Isono
Ultrastructural changes of the parathyroid glands of isoproterenol-treated golden hamsters were investigated. Many chief cells in the parathyroid glands after 5 and 10 minutes of administration of isoproterenol contain well-developed Golgi complexes and granular endoplasmic reticulum, numerous prosecretory granules, and many secretory granules in the peripheral cytoplasm as compared with the control animals. Many chief cells in the parathyroid glands after 1,3,6 and 12 hours of administration have poorly-developed Golgi complexes, granular endoplasmic reticulum, many secretory granules and numerous lipid droplets as compared with the control animals. The morphology of the parathyroid gland after 30 minutes and 24 hours of administration resembles that of the control animals. It is considered that isoproterenol affects the secretory activity of the parathyroid gland.
Open Access
Maternal smoking during pregnancy affects neuroendocrine cells in the neonate hamster lung
(Murcia : F. Hernández, 1988) Keith, lngegerd; Kary, John R.
Primigravid Syrian golden hamsters were exposed in a Walton smoking machine to the smoke from either weak or strong cigarettes for 10 minute periods, 4 times a day from the 3rd to 14th (2nd last) day of pregnancy. Control hamsters were either similarly restrained in a Walton machine equipped with an unlit cigarette, or were not placed in the machine or restrained. Examination of the progeny in the first 6 days of life showed changes in density indices of grouped pulmonary neuroendocrine (NE) cells (neuroepithelial bodies, NEB) that were related to in utero exposure to maternal smoking. Argyrophil NEB were more numerous, larger, and contained more cells at birth among neonates whose mothers smoked the strong cigarette (2.45 mg nicotine and 36.8 mg tar) during pregnancy. This suggests a doserelated effect as the weak cigarette (0.37 mg nicotine and 33.8 mg tar) group did not show such changes. However, some of the changes described did not last through 3 or 6 days of age. The stress resulting from restraint alone also appeared to increase argyrophil NEB indices. Lung tissue volume fraction was increased in the weak cigarette group over all other groups at birth and 3 days; this suggests that low nicotine has the strongest pharmacological effect on lung tissue growth. The medial thickness of pulmonary arterioles was unchanged by either treatment; this provides morphometric evidence that chronic pulmonary hypertension was not present. We could not determine whether the increased NEB indices were caused by increased stainability, by activation of resident reserve cells, or by actual mitosis.

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