Histology and histopathology Vol. 2, nº 2 (1987)

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http://hdl.handle.net/10201/177470

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High-energy adhering junctional complexes or with mitochondrial coupling
(Murcia : F. Hernández, 1987) González Santander, R.; Martínez Cuadrado, G.; Rubio Sáez, M.
A variety of adhering junction is found in the ependyma of the domestic cat with a coupling of mitochondria. These are symmetrically situated (in mirror form) at both sides of the intercellular cleft, which always maintain the same separating distance, thereby leaving a limiting cellular space of a constant amplitude. The hypothesis is put forward that the energy (ATP), provided by the mitochondria over adhering junctional complexes, would produce separate fields of force which would position in a lengthwise direction the molecules which give rise to the anchoring filaments. The mitochondrial energy provided and the electrostatic forces generated would produce an adhering, intercellular junction which is functionally very strong and which could be called: high-energy adhering junctional complexes or with mitochondrial coupling.
Open Access
Autoradiographic investigation of circadian rhythms in alveolar bone periosteum and cementum in young mice
(Murcia : F. Hernández, 1987) Tonna, E.A.; Singh, I.J.; Sandhu, H.S.
This report presents circadian rhythms in cell proliferation of alveolar bone periosteum and cementum of the maxillary first molars of male 5-week-old BNL, Swiss albino mice which were maintained on a 12 hr light1 dark cycle. Mice were injected with 3H-TDR (lucilgm. body wt.) 1 hr prior to sacrifice and killed every 3 hrs for 24 hrs starting at 9 a.m. Maxillae were decalcified, routinely processed histologically and autoradiographs prepared. Cell labeling indices of alveolar bone and cementum mesial to the first molar were determined. Alveolar bone periosteal and cemental cells show circadian rhythm in their DNA synthetic processes. Peaks in percent labeling exhibit higher values than previously reported for nontraumatized, normal dental periosteum and cementum. While the outer periosteum reveals a single 24 hr peak (6 p.m.), inner periosteum and cementum reveal two ultradian peaks 9 to 12 hrs apart involving both light and dark periods. Rodents are nocturnal, but high peaks are also evident in the light periods, consequently, not all peaks are synonymous with the period of animal activity and feeding. Although the single daylight peak of the outer periosteum may indicate growth of that surface at night to about noon, the double peaks exhibited by inner periosteum and cementum indicate lightldark, continuously active surfaces in terms of DNA synthesis and growth
Open Access
The influence of bromocriptine on the ultrstructure of the biceps femoris muscle in mice
(Murcia : F. Hernández, 1987) Stravoravdi, P.; Polyzonis, M.; Demetriou, Th.
Bromocriptine and other dopaminergic agonists drugs are used in Parkinson's disease. In this paper we have studied the ultrastructure of striated muscle of mice after bromocriptine treatment. There was a tremendous increase in the number and size of mitochondria. as well as a very notable increase in the cristae. Some ultrastructure changes were also noted at the neuromuscular junctions. An explanation has been attempted in the light of other investigations concerning the relationship of microtubules and bromocriptine on the one hand, and microtubules and mitochondria on the other.
Open Access
Endocrine cells of the gastric mucosa of Rana temporaria L.
(Murcia : F. Hernández, 1987) Díaz de Rada, O.; Sesma, P.; Vazquez, J.J.
The endocrine cells of the gastric mucosa of Rana temporaria have been studied according to the ultrastructure, the staining properties of the granules with Masson Fontana's and Grimelius' silver methods, silver impregnation of Davenport on deplasticised semithin sections and immunocytochemical techniques. Seven different types of endocrine cells have been described. Six were regarded as belonging to known types: G, A, EC, ECL, D and P cells. One type was considered as unclassifiable.
Open Access
Classification of routinelyprocessed anaplastic large cell tumours with a small panel of antibodies. An immunohistochemical study
(Murcia : F. Hernández, 1987) Henzen-Logmans, Sonja C.; Mullink, Hendrik; Vennegoor, Clauss; Jo Hilgers, Jan Oort; Meijer, Cris J.L.M.
A proportion of anaplastic large cell tumours is difficult to classify on sections of routinely processed, paraffin-embedded tissue. Differentiation into large cell lymphoma, carcinoma, melanoma or sarcoma is important in order to assess prognosis and proper treatment. Although the use of immunohistochemistry has been reported in the differentiation between some of these types of neoplasms, no antibody panel, which can directly differentiate all of them, has been described. In the present study we evaluated the value of a panel of 5 antibodies for the classification of 29 anaplastic large cell tumours, which could not be classified by experienced pathologists using conventional histological and histochemical techniques. The panel, which can be used on routinely fixed paraffin-embedded tissue, consisted of 5 different antibodies directed against keratin, vimentin, the human milk-fat globule membrane antigen MAM-6, a melanoma associated antigen and common leucoyte antigen. The use of this panel directly resulted in a definite diagnosis in 95% of the cases and provided valuable information for the diagnosis in the remaining cases. The diagnosis was confirmed by additional marker studies and electron microscopy. Moreover, clinical follow-up, including treatment data, was in accordance with the diagnosis based on the panel.

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