Histology and histopathology Vol. 7, nº 3 (1992)
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- PublicationOpen AccessMonoclonal antibody against histiocytosis X cells(Murcia : F. Hernández, 1992) Ishikawa, Makoto; Matsuda, Mikio; lmai, YutakaMonoclonal antibody against histiocytosis X cells (HXCs) was established. The antigen was the cell membrane of HXCs from the submandibular lesion of a 63-year-old man who had been dignosed as an adult type of histiocytosis X (HX) and whose HXCs had numerous Birbeck granules (BGs). The obtained monoclonal antibody, named MI1, reacted with the antigenic cell membrane of HXC. Immunoblotting showed that MI1 bound to the cell membrane of 28500 mw. MI1 also reacted with interdigitating reticulum cells (IDCs) in the tonsil and Langerhans cells (LCs) in the epidermis. MI1 reacted with the BGs which connected to the cell membrane, but not with those located near the nucleus.
- PublicationOpen AccessScanning electron microscopic and light microscopic observations on morphological changes of freeze-dried bone implantation in rats(Murcia : F. Hernández, 1992) Matsuda, Mikio; Satoh, Y.; Ono, K.Bone remodelling after the implantation of freeze-dried autogenous bone in rat parietal bone was compared with fresh autogenous bone transplantation, using a scanning electron and light microscope revealed the time intervals after transplantationfimplantation. The light microscope revealed the time delay of the bone remodelling in the implantation, compared with the transplantations. The scanning electron microscope showed that the differences between the two groups were in the states of bone union and bone resorption. In the fresh bone group, the newly-formed bone filled the spaces between host and the transplanted bones at 2 to 3 weeks after the transplantation: the newly-formed bone fused and melted into the transplanted bone. New bone formation was more dominant on the bone surface in the dura mater side than in the skin side. The union was almost completed at 5 weeks. In freeze-dried bone implantation, the bone union in the contact space was very poor and the implanted bone was mainly covered by the new bone, which developed from the host bone surface in the dura mater side at 2 to 3 weeks after the implantation. What is noteworthy is that bone resorbed areas showing numerous Howship's lacunae were mainly observed on the host bone surface in the vicinity of newly-formed bone. However in freeze-dried bone implantation, the bone resorption was greater on the host and implanted bone surface than that of fresh bone transplantation: the resorption of host bone was considerably larger at certain periods after freeze-dried bone implantation. The present results show that the healing process of freeze-dried bone implantation, even though autogenous bone was used, differed from that of fresh autogenous bone transplantation, and the differences are concerned not only with time sequences but also with qualitative changes. This suggests that the host would have some different responses to the freeze-dried autogenous bone from fresh materials.
- PublicationOpen AccessDistribution of neuropeptide Y-like immunoreactivity in the brain of Salmo salar and Gambusia affinis(Murcia : F. Hernández, 1992) García-Fernández, J.M.; Del Brío, M.A.; Cernuda-Cernuda, R.; Coto, A.; Riera, P.Through the imrnunohistochernical PAP technique, the distribution of irnrnune positive neurons and fibres for an antibody anti-NPY in the encephalon of salrnon fixed in Bouin have been located and studied. NPY-positive neurons are found forming three important nuclei: in the ventrolateral telencephalon; in the tegmentum mesencephali; and in the locus coemleus. Neurons in the optic tecturn, in the thalarnic region and a few in the preoptic recess have also been located. The fibres were found throughout the brain, with the exception of the cerebellurn, presenting a greater density in three regions: in the dorsal telencephalon; in the mesencephalon; and in the visceral lobes in the rhombencephalon. With the airn of proving if this distribution is found in other groups of teleosts, we processed, with the sarne technique, the advanced teleost Gambusia affinis, in order to compare it with the prirnitive teleost Salmo salar. The results show that in both fish this neuropeptide has the same pattem of distribution. The results also suggest that in fish this neuropeptide can be involved in severa1 functions of the central nervous systern, as has been dernonstrated for rnarnmals. The innervation of the visceral lobes and also the presence of NPY-fibres in the posterior hypothalarnus are anatornical supports of the studies which suggest that NPY is related to the control of the food intake.
- PublicationOpen AccessAge-related morphometric changes occurring in the somata of astrocytes of the granular layer of rat neocerebellar cortex (Crus I and Crus II)(Murcia : F. Hernández, 1992) Monteiro, R.A.F.; Rocha, E.; Marini-Abreu, M.M.A quantitative study conceming the agerelated changes occurring in the cell body and somatic organelles of neocerebellar astrocytes is carried out, using rats aged 2 to 24 months. Manual stereological techniques are used to determine the following parameters on electron micrographs: the somatic volume, the volume density and the absolute volume of the protoplasmic structures as well as the mean surface of the rough endoplasmic reticulum cisternae per cell.None of the pararneters reveals any clear-cut general temporal pattern. The soma and the cell components show statistically significant differences in the parameters with ageing, excepting the dense bodies (relative and absolute volumes) and the Golgi apparatus (relative volume). There are significant positive linear trends between, on the one hand, the somatic volume and, on the other hand, the absolute volume of either of the following structures: nucleus, glial filaments, ground substance and dense bodies. Some linear correlations between the absolute volumes of organelles are also found. Despite the ability for karyokinesis, it is concluded that astrocytes do undergo changes with ageing.
- PublicationOpen AccessExpression of HBsAg and HBsAg in liver tissue, correlation with disease activity(Murcia : F. Hernández, 1992) Nakopoulou, Lydia; Adraskelas, N.; Stefanak, K.; Zacharoulis, D.; Hadziyanni, St.The pattems of Hepatitis B surface antigen (HBsAg) and Hepatitis B core antigen (HBcAg) expression were studied in liver biopsies taken from 41 patients with chronic HBV disease. Immunohistochemical methods were used on deparaffinized sections for the identification of HBsAg and HBcAg in liver tissue. lkenty-one of the 41 cases (5 1.2%) were classified as inactive liver disease and 20 (48.8%) as active liver disease. In liver biopsies with inactive disease, HBsAg demonstrated varying types of cytoplasmic expression in a rather high number of hepatocytes distributed mainly in clusters, while HBcAg was rarely expressed in liver nuclei. On the other hand, in liver biopsies with active disease HBsAg was characterized by a diffuse cytoplasmic expression in a few discrete hepatocytes, while HBcAg was expressed in the nuclei of the hepatocytes in 70% of the cases and in half of the positive cases it was also detected in the cytoplasm. In conclusion, HBsAg expression in a few scattered hepatocytes correlates with active liver disease and positive HBcAg, while varying HBsAg cytoplasmic expression in a rather high number of clustered hepatocytes is related to chronic inactive liver disease and negative expression of HBcAg.