Histology and histopathology Vol. 4, nº 4 (1989)
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- PublicationOpen AccessEpidermal Langerhans cells in infectious diseases(Murcia : F. Hernández, 1989) Tapia, F.J.; Cáceres-Dittmar, G.; Acuña, Lourdes; Mosca, Walter
- PublicationOpen AccessThe effects of CDP-choline on newborn rat pups with experimental alcohol fetopathy. A Golgi study(Murcia : F. Hernández, 1989) Patt, Stephan; Cervós-Navarro, J.; Stoltenburg-Didinger, G.; Schreiner, C.Generally accepted features of alcoholic fetopathy are delayed maturation and retarded dendritic development of neocortex, hippocampus and cerebellum. The present study investigates the effects of a membrane stabilizing agent (CDP-choline) on Purkinje cells of chronically alcohol intoxicated newborn rat pups, employing a Golgi impregnation technique. Both quantitative and qualitative data indicate that CDP-choline modifies the alcohol induced lesion.
- PublicationOpen AccessUltrastructural and immunoelectron microscopic studies on infiltrating mononuclear cells in lymphocytic submandibulitis in NOD mice(Murcia : F. Hernández, 1989) Takahide Sugihara; Yasuro Yoshimura; Tanaka, OsamuThe ultrastructural relations of the infiltrating mononuclear cells to the parenchyma1 tissues were studied in the submandibular gland of the female nonobese diabetic (NOD) mouse. In addition, the phenotype of mononuclear cells infiltrating the submandibular , gland has been determined by light and electron microscopy by using monoclonal antibodies against T-cell subsets (Thyl.2, Lytl, Lyt2). Ultrastructurally, lymphoid cells were frequently observed around and in the acini and ducts. Some of the lymphoid cells observed in the acini and ducts were irregular in shape and sometimes sent spike-like projections into acinar and ductal cells. Immunohistochemical study demonstrated that Thyl.2+ cells were predominant among the infiltrating cells, and the majority of these infiltrating T-cells were composed of Lytl+ cells with a small proportion of Lyt2+ cells. By immunoelectron microscopy, lymphocytes carrying Thyl.2, Lytl or Lyt2 antigen were identified, as is demonstrated by an electron-dense reaction product on the entire cell surface, and these immunopositive cells were frequently observed around and in the acini and ducts. Some of the Thyl.2+, Lytl+ or Lyt2+ cells observed in the acini and ducts demonstrated a close contact with acinar and ductal cells and both Lytl+ and Lyt2+ cells sent spikelike projections into them. Occasionally, a partial degeneration of acinar cell adjacent to the invading lymphocytes was observed. These observations suggest that T-lymphocytes are involved in the direct destruction of acinar and ductal cells in the NOD mouse submandibular glands.
- PublicationOpen AccessUltrastructural and hormonal metabolic studies of rat liver maintained in vitro by perfusion at 30° C and 37O C: a time course study by TEM, SEM and RIA(Murcia : F. Hernández, 1989) Hassan, Ibrahim M.; Al-Ali, Saad Y.; Hassan, MithalIsolated rat liver perfusion system has been extensively used for metabolic and functional studies. Results derived from the application of this system may reflect true biochemical changes but they may also be associated with some structural changes. This study was undertaken to correlate the cytological changes and functional integrity of isolated rat liver perfused in vitro at normal physiological temperature (37°C) and 30°C, using a non-recirculating system. The livers were perfused for 3 hours with modified Ham's F10 culture medium supplemented with thyroxine hormone (T4). The hepatocyte structural integrity was studied by light microscopy, transmission and scanning electron microscopy. The triiodothyronine (T3) and T4 hormones in the perfusion medium and the effluent fractions were assessed by radioimmunoassay. The livers perfused at 30°C remained morphologically intact at the ultrastructural level for 3 hours whilst at 37"C, hepatocytes in the centrilobular zone exhibited marked structural alterations. The percentage of T4 uptake was significantly higher (P < 0.01) in livers perfused at 30°C (50.8 * 7.7% vs 38 * 7.7%, 37"C), but the net T3 output (3.16 * 1.04 pg) and the conversion of T4 to T3 (4 * 0.62%) were significantly higher (P < 0.001) in livers perfused at 37°C in comparison to livers perfused at 30°C (1.61 * 0.84 pg and 1.68 2 0.76%, respectively). In conclusion, at 30°C the hepatic T4 uptake is not inhibited, but the rate of T4 to T3 conversion has decreased, additionally the livers remain morphologically well preserved throughout the experimental period. At 37"C, although T4 to T3 conversion is higher, structurally the livers could not be maintained intact for more than 2 hours. Therefore, isolated rat livers perfused in vitro at 30°C offer the best compromise for further morphological and metabolic studies.
- PublicationOpen AccessCompensatory adrenal growth in aldosterone-treated male and female hamsters(Murcia : F. Hernández, 1989) Kasprzak, A.; Malendowicz, L.K.The aim of the study was to investigate the compensatory adrenal growth in aldosterone-treated male and female hamsters. Hemiadrenalectomised and sham-operated animals were treated for 5 days with a daily d-aldosterone dose of 25 pglanimal. In both male and female aldosterone-treated hamsters monoadrenalectomy did not change the relative adrenal weight if compared with sham-operated groups. The fasciculata zonae of monoadrenalectomised aldosterone- treated males was larger and contained more parenchyma1 cells than in appropriate control group. There was no difference in the volume of adrenocortical zones, average cell volume and in cell number between sham-operated and unilaterally adrenalectomised females. In vitro 3H-thymidine incorporation per adrenal was markedly higher in monoadrenalectomised than in sham-operated aldosterone-treated males while the opposite was true for female hamsters. Thus, the action of aldosterone on CAG in the hamster seems to depend on sex, with no effect in males and inhibitory action in females.