Histology and histopathology Vol. 4, nº 4 (1989)
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- PublicationOpen AccessGlomerular filtration barrier in experimental endotoxin shock, a histopathological and physiopathological study(Murcia : F. Hernández, 1989) Redondo, E.; Roncero, V.; Durán, E.; Gázquez, A.In the present work a morphopathological study is carried out of the glomerular filtration barrier in 20 Large-White pigs weighing 20 kg, subjected to experimental intravenous inoculations of endotoxin from Salmonella enteriditis. The study is completed with the determination of protein plasmatic levels, through urine test with the determination of pH, density, proteins, glucose, ketone body levels and urinary sediment. The histopathological and physiopathological results reveal alterations at the level of the filtration barrier with quantitative differences between the different experimental groups.
- PublicationOpen AccessPeripheral T-cell lymphoma or Hodg kin's disease in a HIV seropositive patient: a histopathological study(Murcia : F. Hernández, 1989) Kanavaros, Panagiotis; Nemeth, J.; Lavergne, Anne; Ngo Van, P.; Galian, AnnieMalignant lymphomas occurring in patients with AIDS are usually derived from the B-cell lineage while T-cell malignant lymphomas are very rare in these patients. We report a HIV seropositive 29-year-old homosexual man in whom cervical lymph node biopsy showed an atypical lymphoproliferative process. On morphological and paraffin section immunohistochemical grounds the possibility of Hodgkin's disease (HD) mixed cellularity was initially suggested, but frozen section immunohistochemical studies revealed that the cellular infiltrate exhibited an aberrant pan T immunophenotype and consequently the diagnosis of peripheral T-malignant lymphomas (T-ML) was made. However, genotypic studies would be required to definitely confirm this diagnosis, in such cases. In our case, varying numbers of small and mediumsized cells were positive for both Leu 3lCD4 and Leu 2lCD8 whereas some large cells reacted only with Leu 3lCD4 antibody. Some medium-sized, large and giant cells showed cytoplasmic positivity for Leu MlICD15. Furthermore, the positivity of many large and giant cells with the activation markers BerH2lCD30, Ki-1lCD30, TacICD25 and HLA-DR suggested an activation state for these cells. Our findings emphasize the usefulness of frozen section immunohistochemical methods in order to investigate the spectrum of lymphoid malignancies occurring in HIV seropositive patients, and confirm results of previous studies which stressed the diagnostic difficulties that may appear in distiguishing HD from peripheral T-ML.
- PublicationOpen AccessCompensatory adrenal growth in aldosterone-treated male and female hamsters(Murcia : F. Hernández, 1989) Kasprzak, A.; Malendowicz, L.K.The aim of the study was to investigate the compensatory adrenal growth in aldosterone-treated male and female hamsters. Hemiadrenalectomised and sham-operated animals were treated for 5 days with a daily d-aldosterone dose of 25 pglanimal. In both male and female aldosterone-treated hamsters monoadrenalectomy did not change the relative adrenal weight if compared with sham-operated groups. The fasciculata zonae of monoadrenalectomised aldosterone- treated males was larger and contained more parenchyma1 cells than in appropriate control group. There was no difference in the volume of adrenocortical zones, average cell volume and in cell number between sham-operated and unilaterally adrenalectomised females. In vitro 3H-thymidine incorporation per adrenal was markedly higher in monoadrenalectomised than in sham-operated aldosterone-treated males while the opposite was true for female hamsters. Thus, the action of aldosterone on CAG in the hamster seems to depend on sex, with no effect in males and inhibitory action in females.
- PublicationOpen AccessMuscle regeneration induced by snake venom. A histological and histochemical study(Murcia : F. Hernández, 1989) Peña, J.; Jimena, I.; Luque, E.; Martín, J.D.; Vaamonde, R.This report describes the regeneration pattern of anterior tibial muscle of the rat after the inoculation of the snake venom of Bothrops jararacussu . The results show that this regeneration pattern is rather similar to the pattern described in other experimental models. Three days after the injection, three differentiated areas are established: a periferic one of surviving fibres, a second one called myogenic area, and the last one, more internal, made of necrotic fibres that are phagocited by macrophages. The surface of the surviving muscle fibres has myoblasts sticking to it and five days after, the myogenic area is occupied by many of them. Both the previous phagocytosis and the myoblasts came from the area of current uninjured fibres. After 30 and 60 days the regeneration is completed and there are only a few marks that show that the regeneration has taken place.
- PublicationOpen AccessNucleolar organiser regions in colonic dysplasia. A preliminary study(Murcia : F. Hernández, 1989) Sutherland, C.G.G.; McMeekin, W.As a preliminary investigation in the evaluation of the argyrophilic nucleolar organiser region (AgNOR) technique in colonic dysplasia, quantitation of AgNORs was carried out in biopsies of normal rectal mucosa and tubulovillous adenomas. The AgNOR counts in the lower third of the normal crypts were approximately twice those in the surface mucosa but there was no significant difference between counts in normal crypt bases and adenomas. It is concluded that the AgNOR technique is unlikely to be of value in the assessment of colonic dysplasia.