Histology and histopathology Vol.12, nº 1 (1997)
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- PublicationOpen AccessPrognostic analysis of astrocytic gliomas correlating histological parameters with the proliferating cell nuclear antigen labelling index (PCNA-LI)(Murcia : F. Hernández, 1997) Cruz-Sánchez, F. F.; Ferreres, Joan-Carles; Figols, J.; Palacín, A.; Cardesa, Antonio; Rossi, M.L.; Val-Bernal, José FernandoEighty out of 250 cases of astrocytic glioma collected from a practice served by a single clinical team over a 15-year period were studied using a full complement of clinical, follow up, histopathological analysis and proliferating cell nuclear antigen (PCNA) immunostaining for the obtention of the PCNA-labelling index (LI). A statistical evaluation and discriminant analysis were carried out with the aim of clarifying the importance of various parameters as predictors of tumor behaviour. Data are correlated with survival (with a 10- year follow up). A significant correlation with survival was found when histological grouping and the PCNA-LI were studied with the Cox test. Most significant features were histological as detected using classical techniques including histological grading. The utilization of objective values (mitosis, cellular density and necrosis) appears to be useful in grading astrocytic tumors. Our results emphasize the importance of cytological, histological and PCNA-LI parameters as predictors of tumor behaviour.
- PublicationOpen AccessK-FGF protoncogene expression is associated with murine testicular teratogenesis, but is not involved during mouse testicular development(Murcia : F. Hernández, 1997) Anta, J.M.de; Monzó, M.; Peris, B.; Ruano, D.The k-FGF gene, which belongs to the family of the fibroblast growth factor genes, is implicated in tumoral and developmental processes. It is expressed in embryonal carcinoma cells, in embryonic stem cells, during limb and tooth formation and in some germ cell tumors. However, the expression of this protooncogene during testicular development as well its relationship to spontaneous teratogenesis have not been determined. Here we investigate k-FGF expression during testicular development in mice, as well as in a spontaneous testicular teratoma (STT) and in the OTT6050 teratocarcinoma (TC) by Northern blotting, RT-PCR and in situ hybridization. Severa1 data indicate that k-FGF gene contains downstream regulatory sequences which bind octamer factors. One of these transcription factors which binds to k-FGF enhancer is Oct-4. Although the k-FGF gene is activated by Oct-4 in embryonal carcinoma and embryonic stem cells and Oct-4 is expressed in the germ cells of the embryo, our results indicate that there is no detectable k-FGF expression in mouse testicular germ cells at any stage of development. This indicates that Oct-4 does not activate transcription of the k-FGF gene in mouse germ cells, and that k-FGF is not implicated during testicular development. We also show that there is a high k-FGF expression in the experimental OTT6050 TC, but only very low levels in a murine differentiated STT, suggesting that k-FGF activation may be responsible for the genesis and development of STT, behaving as a marker of malignancy in these neoplasms .
- PublicationOpen Accesslmmunohistochemical expression of Bcl-2 oncoprotein in EBV-associated nasopharyngeal carcinoma correlated to histological type and survival(Murcia : F. Hernández, 1997) vera-Sempere, F.J.; Burgos, J.S.; Botella, M.S.; Morera, C.Expression of Bcl-2 is associated with inhibition of apoptosis and extension of cell survival. In vitro Bcl-2 protein expression is up-regulated by the EBV-latency associated antigen latent membrane protein (LMP-1). We have investigated the relationship between the presence of EBV-DNA screened by means of sensitive nested-PCR, nasopharyngeal carcinoma (NPC) histological types according to two different schemata (WHO and Micheau classifications) and Bcl-2-124 immunohistochemical expression in 55 biopsy samples of NPC. EBV genome was detected in 100% of samples with sufficient DNA quality to support the previous view that al1 types of NPC are variants of EBV-infected neoplasia. Bcl-2 was observed in the basa1 layer of normal nasopharyngeal mucosa and also at cytoplasmic level in 42 of 55 (76.4%) NPC cases. Mitotic neoplastic cells usually showed strong cytoplasmic and chromosomal staining, a finding not well referred to previously. Bcl-2 expression was significantly associated (p<0.05) to undifferentiated NPC (UNPC) when a histological classification with only two major microscopical types was applied. No close correlations were found between the presence of EBV-DNA, NPC location, clinical stage and age or sex of the patients in relation to Bcl-2 positive expression. However, when comparing Bcl-2 expression and known survival mean of the patients, significant differences were observed (p<0.001) so that mean survivals were 3 1.1, 24.4, 52.2 and 54.1 months respectively for NPC patients with -, +, ++ and +++ Bcl-2 immunoreactivity. Nevertheless this better clinical outcome in Bcl-2 NPC positive cases may depend on the histological type due to close relationship with UNPC. Only studies of larger series with long-term follow-up and multivariate analyses may document whether Bcl-2 expression is an independent prognostic marker in the evolution of NPC patients.
- PublicationOpen AccessCharacterisationof thyroid medullary carcinoma TT cell line(Murcia : F. Hernández, 1997) Zabel, M.; Grzeszkowak, J.-
- PublicationOpen AccessUltrastructural characteristics of blood vessels in the infant and adult human cerebral cortex(Murcia : F. Hernández, 1997) Zhang, H.F.; Ong, W.Y.; Leong, S.K.; Garey, L.J.Blood vessels in frontal and temporal cerebral cortex of adults and two infants aged 5 months and 5 years were studied by electron microscopy. The cells outside the endothelium were classified on their ultrastructural characteristics. Fibroblasts had prominent rough endoplasmic reticulum and few mitochondria in the cytoplasm. They were different from pericytes, which contained a prominent Golgi apparatus but only a few, isolated profiles of rough endoplasmic reticulum. Smooth muscle cells were distinguished from fibroblasts and pericytes by the presence of filaments and caveolae. Perivascular cells were characterised by the presence of lysosomes and granules of different sizes and electron densities, and were present at al1 ages studied. Plasma cells had abundant rough endoplasmic reticulum in the cytoplasm, and were present only in the 5-month-old infant cortex. Cortical vessel diameter increased with age.