Browsing by Subject "Microscopy"

Now showing 1 - 6 of 6
  • Thumbnail Image
    Publication
    Open Access
    Anatomical and histochemical study of Sechium edule (Jacq.) Sw.
    (Universidad de Murcia. Servicio de publicaciones, 2020) de Andrade Silva, Francilainy Karina; dos Santos Magalhães, Cledson; Damasceno Sá, Rafaela; Lins da Silva, Flávia Carolina; Perrelli Randau, Karina
    Sechium edule (Jacq.) Sw. tiene un papel importante nutricional y terapéuticamente en la medicina popular, especialmente utilizado como agente hipotensor. Para ampliar la información farmacológica sobre esta especie, este trabajo tiene como objetivo el estudio histoanatómico de sus hojas. Se prepararon muestras con secciones transversales y paradérmicas de la hoja para el estudio microscópico. Para el análisis histoquímico, se utilizaron reactivos específicos para los metabolitos objetivo. A través del análisis realizado se identificaron estructuras anatómicas para la correcta identificación de la especie estudiada. La histoquímica mostró la presencia de alcaloides, triterpenos y esteroides, compuestos lipofílicos y lignina. Esta información sobre la localización de los compuestos no tiene precedentes. Así, el trabajo amplía el conocimiento sobre la especie y la familia Cucurbitaceae.
  • Thumbnail Image
    Publication
    Open Access
    Cadmium- and lead-induced apoptosis in mallard erythrocytes (Anas platyrhynchos)
    (Elsevier, 2009-01) Romero García, Diego; Hernández-García, A.; Tagliati, C. A.; Martínez-López, E.; García Fernández, Antonio Juan; Ciencias Sociosanitarias
    Cadmium, lead and cadmium–lead (1:10) induced apoptosis were studied using mallard blood cells. The allowable range in concentrations were: 0.01–0.5, 0.1–5.0, and 0.01:0.10–0.50:5.00 mM, for cadmium, lead and cadmium–lead, respectively. The lowest EC50 achieved was for cadmium (0.2270.04 mM). Two doses from each treatment group were chosen to study apoptosis and the presence of metals in cells. The percentage of apoptotic cells increased as the concentration of metals increased. The percentage of cells with intracellular metals was high for both exposure levels and the quantity of intracellular metal was greater for exposure to high concentrations. Morphological alterations for all types of exposure were related to the diverse range of effects that these metals have on membranes. We suggest that the decrease in the number of erythrocytes observed in specimens suffering from lead and cadmium poisoning is related to the induction of apoptosis.
  • Thumbnail Image
    Publication
    Open Access
    CD34+ stromal cells/fibroblasts/fibrocytes/telocytes as a tissue reserve and a principal source of mesenchymal cells. Location, morphology, function and role in pathology
    (F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología, 2014) Díaz-Flores, L.; Gutiérrez, R.; García, M. P.; Sáez, F.J.; Díaz-Flores Jr., L.; Valladares, F.; Madrid Cuevas, Juan Francisco
    We review the morphofunctional characteristics of CD34+ stromal fibroblastic/fibrocytic cells (CD34+ SFCs) and report our observations. We consider the following aspects of CD34+ SFCs: A) The confusing terms applied to this cell type, often combining the prefix CD34 with numerous names, including fibroblasts, fibrocytes, dendrocytes, keratocytes, telocytes and stromal, dendritic, adventitial, supraadventitial, perivascular, paravascular and delimiting cells; B) Changes in their immunophenotype, e.g., loss of CD34 expression and gain of other markers, such as those defining mesenchymal and derivate cells (myofibroblasts, osteoblasts, chondroblasts, adipocytes); C) Morphology (elongated or triangular cell body and thin, moniliform, bipolar or multipolar cytoplasmic processes), immunohistochemistry (co-expression of and changes in molecular expression) and structure (characteristics of nucleus and cytoplasmic organelles, and points of contact and junctions in quiescent and activated stages by light and electron microscopy); D) Location and distribution in the vessels (adventitia or external layer), in the tissues (connective, adipose, blood, muscle and nervous) and in the organs and systems (skin, oral cavity and oropharynx, respiratory, digestive, urinary, male, female, endocrine and lymphoid systems, serosal and synovial membranes, heart, eye and meninges); E) Origin from the mesoderm and cranial neural crest in the embryo, and from stem cells (themselves or other cells) and/or peripheral blood pluripotent stem cells (circulating progenitor cells) in post-natal life; F) Functions, such as synthesis of different molecules, progenitor of mesenchymal cells, immunomodulation, parenchymal regulation (growth, maturation and differentiation of adjacent cells), induction of angiogenesis, scaffolding support of other cells and phagocytic properties. Since CD34+ SFCs are the main reservoir of tissue mesenchymal cells (great mesenchymal potential, probably higher than that proposed for pericytes and other stromal cells), we dedicate a broad section to explain their in vivo behaviour during proliferation and differentiation in different physiologic and pathologic conditions, in addition to their characteristics in the human tissues of origin (adult stem cell niches); G) Involvement in pathological processes, e.g., repair (regeneration and repair through granulation tissue), fibrosis, tumour stroma formation and possible CD34+ SFC-derived tumours (e.g., solitary fibrous tumour, dermatofibrosarcoma protuberans, giant cell fibroblastoma, nuchal-type fibroma, mammary and extramammary myofibroblastoma, spindle and pleomorphic cell lipoma, and elastofibroma) and H) Clinical and therapeutic implications.
  • Thumbnail Image
    Publication
    Open Access
    Comparison of cytopathological changes induced by mercury chloride exposure in renal cell lines (VERO and BGM)
    (Elsevier, 2004-03-31) Romero García, Diego; Gómez Zapata, Maximiliano; Luna, Aurelio; Ciencias Sociosanitarias
    The response to mercury chloride was assessed in two cell lines of renal origin, determining the range of toxic concentrations by Neutral Red assay after 24-h of exposure. Morphological changes in the Buffalo Green Monkey (BGM) and VERO cell lines after exposure to subcytotoxic doses (0.045 and 0.038 mM, respectively) equivalent to EC10 (effective concentrations 10%) of mercury chloride were evaluated at the structural and ultrastructural level by optic, transmission and scanning microscopy. Using transmission electron microscopy, the most notable findings in treated cells were the presence of intracytoplasmic inclusion bodies and apoptotic bodies. Scanning microscopy pointed to a cell with a disrupted perinuclear region and a decreased number of surface microvilli. Similar alterations in both in vivo and in vitro experiments have been described by other authors. We conclude that BGM and VERO renal cell lines can be considered as useful tools for toxicological studies involving mercury chloride.
  • Repository logo
    Publication
    Open Access
    Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
    (Elsevier, ) Albaladejo-Riad, Nora; Espinosa-Ruiz, Cristóbal; Esteban Abad, María de los Ángeles; Biología Celular e Histología
    Silk fibroin microparticles (SF) were administered in the diet of gilthead seabream with or without experimental skin wounds to study the effects on cellular immunity and liver. A commercial diet was enriched with different SF contents: 0, 50 and 100 mg kg-1 (control, SF50 and SF100 diet, respectively). The animals were fed for 30 days and half of them were sampled. Similar experimental wounds were then performed to the rest of fish and they continued to be fed the same diet. At 7 days post-wounding, samples were taken from the wounded fish. Cellular immunity was studied on head kidney leucocytes (phagocytosis, respiratory and peroxidase content) and liver status (histological study and gene expression) were studied. Our results showed that experimental wounds affect both cellular immunity (by decreasing leucocyte respiratory burst and peroxidase activity) and altered liver histology (by inducing vascularization and congestion of blood vessels). In addition, it affects the expression of genes indicative of oxidative stress, endoplasmic reticulum stress and apoptosis. More specifically, it was the highest dose of silk fibroin (SF100) that significantly increased the phagocytic capacity of leucocytes as well as vascularization in the liver. In addition, increased expression of antioxidant genes (cat and gsr) and decreased expression of genes related to reticulum endoplasmic stress (grp94 and grp170) and apoptosis (nos and jnk) were detected in these fish fed with SF100 and wounded. In conclusion, fed fish with SF100 had many beneficial effects as cellular immunostimulant and hepatoprotection in wounded fish. Its use could be of great interest for stress management in farmed fish conditions.
  • Thumbnail Image
    Publication
    Open Access
    Morphoanatomical and histochemical study of Ipomoea hederifolia L. (Convolvulaceae)
    (Universidad de Murcia. Servicio de publicaciones, 2023) Vital dos Santos, Edinalva Alves; Nurit Silva, Kiriaki; Pereira de Arruda, Emília Cristina; Leite, Ana Virginia
    Ipomoea hederifolia L. is a herbaceous vine native to the tropical Americas with important medicinal properties. Was realized a pharmacobotanical study of the leaves and stems of this species, performing macroscopic and microscopic morphodiagnoses and histochemical tests. Anatomical characteristics typical of the family Convolvulaceae were found. However, the epidermis and its appendages (e.g. striated cuticle and peltate trichomes) and the anatomy of the petiole and the stem presented relevant characters for the taxonomic recognition of the species. Histochemical tests evidenced the presence of lignin and cutin and positive reactions for starch, phenolic compounds, and proteins. The anatomy and the histochemical tests indicated a set of characteristics relevant to the pharmacobotanical characterization of I. hederifolia, expanding our knowledge of the species and providing subsidies for the quality control of its vegetal products.