Publication:
Comparison of cytopathological changes induced by mercury chloride exposure in renal cell lines (VERO and BGM)

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7-Romero_et_al_2004_b (1).pdf(1.39 MB)
Date
2004-03-31
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Authors
Gómez Zapata, Maximiliano ; Luna Maldonado, Aurelio ; Romero García, Diego
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Publisher
Elsevier
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Ciencias Sociosanitarias
DOI
http://dx.doi.org/10.1016/j.etap.2004.03.007
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info:eu-repo/semantics/article
Description
©2004. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted, version of a Published Work that appeared in final form in Environmental Toxicology and Pharmacology. To access the final edited and published work see http://dx.doi.org/10.1016/j.etap.2004.03.007
Abstract
The response to mercury chloride was assessed in two cell lines of renal origin, determining the range of toxic concentrations by Neutral Red assay after 24-h of exposure. Morphological changes in the Buffalo Green Monkey (BGM) and VERO cell lines after exposure to subcytotoxic doses (0.045 and 0.038 mM, respectively) equivalent to EC10 (effective concentrations 10%) of mercury chloride were evaluated at the structural and ultrastructural level by optic, transmission and scanning microscopy. Using transmission electron microscopy, the most notable findings in treated cells were the presence of intracytoplasmic inclusion bodies and apoptotic bodies. Scanning microscopy pointed to a cell with a disrupted perinuclear region and a decreased number of surface microvilli. Similar alterations in both in vivo and in vitro experiments have been described by other authors. We conclude that BGM and VERO renal cell lines can be considered as useful tools for toxicological studies involving mercury chloride.
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BGM and VERO cells line , Cytotoxicity , Mercury , Ultrastructural alterations , Microscopy
Citation
Environmental Toxicology and Pharmacology 17 (2004) 129–141
URI
http://hdl.handle.net/10201/140184
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