Browsing by Subject "Glomerular"

Now showing 1 - 4 of 4
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    Electron microscopic histochemical and immunochemical analyses of heparan sulfate proteoglycan distribution in renal glomerular basement membranes
    (Murcia : F. Hernández, 1991) Rada, Jody A.; Carlson, E. C.
    Renal glomerular basement membranes (GBMs) exhibit a charge-selective barrier, comprised of anionic sites, that restrict the passage of anionic molecules into the urine. These sites are located primarily in the laminae rarae interna (LRI) and externa (LRE) of the GBM and consist of heparan sulfate proteoglycan (HSPG). Previous efforts to localize HSPG core protein within various layers of the GBM have been contradictory. In the present study when rat renal cortex blocks were treated by immersion with the cationic probe, polyethyleneimine (PEI), GBMs exhibited anionic sites concentrated primarily in the LRE and more irregularly within the LRI and lamina densa. All sites were heparitinase sensitive indicating that PE1 positive sites represent negatively charged groups associated with heparan sulfate. In order to gain information on the distribution of the HSPG protein core, antibodies to HSPG from the EHS tumor matrix [anti-(EHS) HSPG] and GBMs [anti-(GBM) HSPG] were used together with immunogold to label thin sections of Lowicryl embedded kidney cortex. Depending upon the antisera used, markedly different distributions of HSPG were obtained. Immunolabelling with anti-(GBM) HSPG suggested a distribution of HSPG which was restricted to the laminae rarae, whereas labelling with anti-(EHS) HSPG indicated that the protein core penetrates through all layers of the GBM.
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    Glomerular histopathology of the contralateral kidney in experimental unilateral hydronephrosis
    (Murcia : F. Hernández, 1987) Moyano, M.C.; Gázquez, A.; Redondo, E.; Roncero, V.
    The aim of this study was to examine the structural, ultrastructural and morphometric alterations which take place in the contralateral kidneys of rats with experimental unilateral hydronephrosis. 20 Wistar rats weighing 250 gr., affected by a process of unilateral hydronephrosis following the ligature of the ureter, were used; these rats were then killed 40,50,60, or 70 days after the ligature. Among the perceived alterations, were immunoglobulin G deposits shown by positive immunoperoxidase reaction and increase in the size of the glomerular and corpuscle from around the fortieth day, and structural alterations that included the pedicels, electrondense deposits in the podocytes and pseudogranular structures in the basal membrane of the capillary.
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    Glomerular pathology in surviving pigs experimentally infected with african swine fever virus
    (Murcia : F. Hernández, 1991) Martín Fernández, José Javier; Igual, A.; Rueda, A.; Sánchez-Vizcaino, J.M.; Alonso-Martí, C.
    Twelve miniature pigs were inoculated with an attenuated African swine fever virus to study glomerular involvement in surviving pigs. In acute phase. kidneys were severely affected and displayed a glomerular capillary thrombosis with fibrin deposition in vascular lumen, detected by immunofluorescence. Fibrin-positive deposits were progressively cleared between one to three months after infection in surviving pigs. The histological picture in kidneys of surviving pigs, up to one post-infection year, showed a focal and segmental glomerulonephritis with hyalinosis, and IgM and C3 deposition was detected by immunofluorescence. Its pathogeny as an evolutive stage of acute glomerular injury is pointed out.
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    High resolution SEM analysis of acellular glomerular basement membrane following pepsin digestion, intrinsic fibrillar structures
    (Murcia : F. Hernández, 1990) Berger, Walter J.; Carlson, E. C.
    Microdissection of acellular rat renal cortex with pepsin was carried out to investigate the morphological substructure of glomerular basement membrane (GBM) by high resolution SEM. Renal cortical blocks (< 5 mm ) from adult male Sprague Dawley rats were rendered acellular by sequential detergent extraction and digested up to 184 hrs with 5 mglml pepsin (185 U/ mg) in 0.5 M acetic acid (pH 2) at 10-15°C. Samples were conventionally prepared for SEM, and observed at original magnifications of 500-100,000 diameters. At low magnifications (500-5,00Ox), acellular GBM surfaces appeared smooth at al1 digestion times. At higher magnifications (50,000-100,00Ox), control GBM surfaces were finely granular. Granule diameter ranged from 20-80 nm, with most between 30-40 nm. Pepsin digestion did not affect average granule size. Beginning at 44 hrs of digestion, intrinsic fibrillar structures comprised of linear arrays of 20-40 nm granules were observed onlin GBM surfaces. At later incubation times, this component of GBM became more extensive. At 160 hrs, the fibrillar arrays frequently bifurcated and showed distinctive «forked» termini, some of which comprised two sides of a triangle (120-150 nm on a side). Fork «handles» (310-350 nm in length) radiated from each angle of the triangle. These sometimes terminated in large granules (approximately 100 nm in diameter), two of which appeared to connect fibrillar arrays end-toend. Together with other arrays, the interconnected triangles appeared to comprise a three-dimensional meshwork extending into the GBM and possibly providing support for, its granular components.