Publication: Electron microscopic histochemical and immunochemical analyses of heparan sulfate proteoglycan distribution in renal glomerular basement membranes
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Date
1991
Authors
Rada, Jody A. ; Carlson, E. C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Renal glomerular basement membranes
(GBMs) exhibit a charge-selective barrier, comprised
of anionic sites, that restrict the passage of anionic
molecules into the urine. These sites are located
primarily in the laminae rarae interna (LRI) and externa
(LRE) of the GBM and consist of heparan sulfate proteoglycan
(HSPG). Previous efforts to localize HSPG core
protein within various layers of the GBM have been
contradictory. In the present study when rat renal cortex
blocks were treated by immersion with the cationic
probe, polyethyleneimine (PEI), GBMs exhibited
anionic sites concentrated primarily in the LRE and
more irregularly within the LRI and lamina densa. All
sites were heparitinase sensitive indicating that PE1
positive sites represent negatively charged groups
associated with heparan sulfate. In order to gain
information on the distribution of the HSPG protein
core, antibodies to HSPG from the EHS tumor matrix
[anti-(EHS) HSPG] and GBMs [anti-(GBM) HSPG]
were used together with immunogold to label thin
sections of Lowicryl embedded kidney cortex. Depending
upon the antisera used, markedly different distributions
of HSPG were obtained. Immunolabelling with
anti-(GBM) HSPG suggested a distribution of HSPG
which was restricted to the laminae rarae, whereas
labelling with anti-(EHS) HSPG indicated that the
protein core penetrates through all layers of the GBM.
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