Browsing by Subject "Genotoxicity"

Now showing 1 - 3 of 3
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    Cytototoxic effects of paracetamol (acetaminophen) in Allium cepa L. meristematic cells and seed germination
    (Universidad de Murcia. Servicio de publicaciones, 2025) Ortiz Molina, Gisele; Gouvea Buchweitz, Jéssica; Aguiar, Rosiane Borba de
    El desecho de medicamentos en la basura doméstica puede ser muy peligrosa para el medio ambiente. El presente trabajo buscó evaluar parámetros de toxicidad del contaminante emergente paracetamol. En los ensayos se utilizaron tres dosis de paracetamol (0,4 mg L-1, 0,5 mg L-1 y 0,6 mg L-1). Los resultados mostraron que la exposición aguda al paracetamol induce varios daños clastogénicos y aneugénicos en el test de A. cepa. No se encontró ningún efecto en la germinación. Aunque el paracetamol es un fármaco importante, también puede representar un riesgo significativo para la producción agrícola y la preservación del medio ambiente. Los resultados del presente trabajo corroboran datos anteriores y refuerzan que la exposición a contaminantes farmacéuticos puede representar un problema significativo para la producción de alimentos, el equilibrio ambiental y la preservación de especies.
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    Evaluation of tissue response and genotoxicity of poly(L/D-lactic acid) disks implanted in rat calvarium
    (F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología, 2012) Gonçalves Göelze, Juliana; Linard Avelar, Rafael; do Nascimento Dolzan, André; Emmel Becker, Otávio; Haas Junior, Orion Luiz; de Oliveira, Luciane Yumi Suzuki; de Oliveira, Rogério Belle
    The aim of the present study was to evaluate local and cytotoxicity systemic tissue reaction in the skull of rats using the implantation of disks of poly (lactic L/D-acid) and evaluate its genotoxicity. 25 males Wistar rats were used, 20 animals underwent surgical procedures and had the discs implanted in the parietal bone, and 5 animals received postoperative medication in the same way, serving as a control group for genotoxicity. The results were subjected to statistical evaluation by analysis of variance (ANOVA). In histological evaluation, between periods of 90 and 120 days in the control group, a new formation at the edges of the defect was noticed. In the experimental group, there was new bone formation at the edges of the defect, migrating below the site occupied by the disk, an absence of inflammatory infiltrate. Regarding the evaluation of genotoxicity, a significant reduction in the frequency of polychromatic erythrocytes in relation to negative control or significant increase in the polychromatic erythrocytes with micronuclei was not detected. So, the material used in this study is biocompatible and well tolerated by the tissues studied, and found to be negative for chromosomal mutagenicity.
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    Histological changes and Micronucleus induction in the Zebra mussel Dreissena polymorpha after Paraquat exposure
    (Murcia : F. Hernández, 2006) Mantecca, P.; Vailati, G.; Bacchetta, R.
    The herbicide paraquat (PQ), still widely used in developing countries, represents a serious risk factor for human and environmental health. To test the sublethal effects of PQ on the freshwater bivalve Dreissena polymorpha, mussels were exposed to 0.125, 0.250, 0.500 mg/L for 7 and 14 days and histologically screened. PQ’s genotoxic potential was also determined in haemocytes by the micronucleus, MN, assay. At concentrations ³ 0.250mg/L, severe lesions, such as cellular vacuolation, lysis and thinness of the germinative epithelia were observed in the digestive gland and testis. A positive trend between the number of granulocytes and all PQ concentrations was observed in both gonads and digestive glands, addressing the inflammatory capacity of this herbicide on these tissues. Mussels exposed to PQ also exhibited a significant MN induction. The spontaneous MN frequencies ranged from 2.75 to 4.25‰, while PQ-induced MN rates in treated mussels were between 3.50 and 12.50‰. The histopathological effects on the digestive and reproductive systems, as well as the MN induction in the haemocytes, confirmed the cytotoxic and genotoxic effects of PQ also in D. polymorpha.