DigitalUM :: Browsing by Subject "Dog"

Browsing by Subject "Dog"

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Open Access
Activation of matrix metalloproteinase (MMP)-2 by membrane type 1-MMP and abnormal immunolocalization of the basement membrane components laminin and type IV collagen in canine spontaneous hemangiosarcomas
(Murcia : F. Hernández, 2009) Murakami, M.; Sakai, Hidetaka; Kodama, A.; Mori, T.; Yanai, T.; Maruo, K.; Masegi, T.
We performed immunohistochemical investigation of the basement membrane (BM) components, namely, type IV collagen and laminin, in 83 canine hemangiosarcomas (HSAs), 22 hemangiomas, and some granulation tissues (GTs). Additionally, we analyzed the expression and activities of matrix metalloproteinase (MMP)-2, MMP-9, and membrane type 1-MMP (MT1-MMP) using the same samples by immunohistochemistry and gelatin zymography to investigate whether MMPs were associated with the BM degradation. In immunohistochemistry for the BM components, many HSAs showed discontinuous linear/negative immunoreactivity in the BM (type IV collagen: 49.4%/14.5%, laminin: 60.3%/10.8%, respectively). In contrast, almost all hemangiomas showed continuous staining in the BM (type IV collagen: 90.9%, laminin: 95.5%, respectively). Interestingly, positive cytoplasmic immunoreactivity for type IV collagen and laminin was observed in 97.6% and 91.6% HSA, respectively. Although MMP-9 immunoreactivity wasn’t detected in neoplastic and active angiogenic endothelial cells (ECs), MMP-2 was detected in all ECs of GTs and in neoplastic cells of both vascular tumors. A strong immunoreactivity for MT1- MMP was observed in active angiogenic ECs in GTs and in neoplastic ECs in HSAs. However, almost all hemangiomas showed weak/negative immunoreactivity. In gelatin zymography, significantly strong activity of active MMP-2 was observed in HSAs, similar to that in active angiogenesis in GTs; however, weak/no activity of active MMP-2 was detected in hemangiomas. In canine HSA, neoplastic cells had active MMP-2, possibly activated by MT1-MMP, and discontinuous status of BM might be associated with activity of active MMP-2.
Open Access
An immunohistochemical study of cyclooxygenase-2 expression in canine multicentric lymphoma
(Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2011) Rodrigues, L.C.S.; Cogliati, B.; Guerra, J.L; Dagli, M.L.Z.; Lucas, S.R.R.
Cyclooxygenase-2 (COX-2) expression has been associated with development and progression in spontaneous human and dogs tumors. Studies demonstrated that non-steroidal anti-inflammatory drugs, which inhibit COX enzyme, may be used in the treatment of some tumors. Lymphoma is a systemic disease and the most common hematological malignancy in dogs. There are few studies about COX-2 expression in human and canine lymphoma. In this study, immunohistochemical evaluation for COX-2 expression was performed in 12 dogs with multicentric lymphoma. The diagnosis was confirmed by histopathological examination. Six samples of normal lymph nodes were either used in the study. No COX-2 immunoreactivity was detected in all samples from canine lymphoma and normal lymph nodes, as well previous studies in canine lymphoma.
Open Access
Apoptotic cell death in canine hair follicle
(Murcia : F. Hernández, 2005) Pascucci, L.; Pedini, V.; Parillo, F.; Gargiulo, A.M.
Apoptotic cell death is an essential homeostatic mechanism involved in the control of cellular turnover in a variety of adult tissues. Cytoplasmic and nuclear condensation morphologically define this process whose biochemical hallmark is extensive DNA fragmentation into discrete oligonucleosomic units. Hair follicle growth and regression has been shown to be correlated with apoptosis in humans, mice, rats and guinea pigs. The present study was carried out to evaluate its implication in canine hair biology in order to define the spatio-temporal relationship between apoptosis and the hair cycle in dogs. As assessed by terminal deoxy-nucleotidyl transferase-mediated d-UTP nick-end-labelling (TUNEL) and by basic histological and ultrastructural assays, apoptotic cells appeared both in the growing and in the regressing follicle epithelium showing the well characterized morphological features described in the previous relevant literature.
Open Access
Avances quirúrgicos en la luxación de rótula: prótesis del surco troclear (a propósito de 2 casos clínicos)
(Facultad de Veterinaria y el Servicio de Publicaciones de la Universidad de Murcia, 2023) Córdoba Guerrero, Álvaro; Murciano Pérez, José; Navarro Alberola, Antonio
La luxación de rótula medial es una patología congénita que afecta al miembro pelviano de perros de razas ”toy” y miniatura que en los últimos años viene experimentado un aumento de su incidencia en razas de gran tamaño. Las técnicas quirúrgicas más frecuentes se han basado en la profundización del surco troclear y la alineación con la tuberosidad tibial, obteniendo resultados subóptimos especialmente en los casos con osteoar-trosis avanzada o deformaciones femorotibiales excesivas.Por ello, este trabajo pretende poner de manifiesto las utilidades y limitaciones que plantea la prótesis del surco troclear (PGR KYON®), por sí misma y en combinación con osteotomías femorales, frente a otras téc-nicas más usadas en base a 2 casos clínicos de luxación de rótula medial complejos. Ambos casos presentaron osteoartrosis asociada a deformidad femoral en varo las cuales se resolvieron quirúrgicamente con diferentes técnicas en función de la edad y el tamaño de los pacientes.La prótesis del surco troclear se presentó como una técnica resolutiva en ambos casos, no obstante, se deben seleccionar cuidadosamente los casos, así como tener en cuenta sus limitaciones, especialmente asociadas a resolución de deformidades angulares femorales.
Open Access
¿Un cánido con alma de león? Un resello para Gran Canaria.
(Revista Numismática Hécate., 2025) García González, Jaime Alberto; Sin departamento asociado
A lo largo del presente trabajo, pretendemos arrojar más luz a la incógnita de partida, donde la numismática canaria ha dado sus primeros, pero decisivos pasos, durante los últimos años. Para alcanzar este objetivo, partiremos de dos hipótesis formuladas en otro trabajo, publicado en 2022, pero que, mediante el análisis de varios ejemplares de este resello grancanario aparecidos a lo largo del 2024, destacaremos dos piezas inéditas, que han resultado ser determinante para la investigación.
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Changes in salivary proteins can reflect beneficial physiological effects of ejaculation in the dog
(2021-01-28) Muñoz-Prieto, Alberto; Escribano, Damián; Horvatić, Anita; Contreras-Aguilar, María Dolores; Bernal, Luis; Rubić, Ivana; Cerón, Jose Joaquín; Dabrowski, Roman; Mrljak, Vladimir; Medicina y Cirugía Animal
The objective of this study was to study the changes in salivary proteins that occur in the dog after the ejaculation process. Saliva samples from eight dogs before and after induced ejaculation were analyzed by proteomic using Tandem Mass Tag (TMT) labeling and LC-MS/MS analysis. A total of 33 salivary proteins showed significant changes after the ejaculation process. The up-regulated proteins that showed changes of higher magnitude were mucin-7 (MUC-7), peroxiredoxin-4 (PRDX4) and galectin-3 (LEGALS3) whereas proteins such as alpha-1-acid glycoprotein (A1G1) and alpha-1B-glycoprotein (A1BG) were the most down-regulated. MUC-7 and PRDX4 expression in saliva after ejaculation could be associated with the protective “environment” created by the organism to exert pr 3o-fertility activities and antioxidants benefits in spermatozoa. Also LEGALS3 increment could be associated with an improvement of wellbeing and could contribute to a positive global effect in the body. Down-regulations of A1G1 and A1GB proteins found in saliva after ejaculation could be associated with a reduction in systemic inflammation. Overall it can be concluded that, changes in proteins in saliva that are produced after ejaculation can reflect a state of increase immune defenses, improvement of antioxidant status and low inflammation.
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Colour and pulsed Doppler Ultrasonographic Study of the canine testis
(Wiley , 2012-07-10) Carrillo Sánchez, J. D.; Soler Laguía, Marta; Lucas Arjona, Xiomara; Agut Giménez, Amalia; Medicina y Cirugía Animal; Facultades de la UMU::Facultad de Veterinaria
Este estudio se llevó a cabo para caracterizar el flujo sanguíneo normal del testículo canino y medir la velocidad sistólica máxima (PSV), la velocidad telediastólica (EDV), el índice de resistencia (RI) y el índice de pulsatilidad (PI) de las arterias testiculares semanalmente durante un período de 6 meses en cinco perros Beagle sanos, así como para evaluar si se producían cambios a lo largo de este tiempo. Los exámenes ecográficos se realizaron con un transductor lineal de 11 MHz. Los vasos testiculares se subdividieron en tres categorías: arterias supratesticulares, arteria marginal y vasos intratesticulares. En las arterias supratesticulares se registraron dos mediciones, en la porción craneal y en la porción en asa (looping). No se observaron diferencias significativas en ninguno de los parámetros estudiados durante los 6 meses que duró el estudio. La porción craneal de la arteria supratesticular mostró un patrón de flujo característico de vaso de alta resistencia, mientras que en la porción en asa de la arteria supratesticular, así como en las arterias marginales e intratesticulares, el flujo presentó un patrón de baja resistencia. Los valores de PSV, RI y PI fueron más elevados en la porción craneal de la arteria supratesticular, seguidos por la porción en asa de la arteria supratesticular, la arteria marginal y los vasos intratesticulares. Las mediciones de EDV fueron mayores en la porción en asa de la arteria supratesticular.
Open Access
Dark-cell areas in the dog vestibular endorgans: an immunohistochemical study
(Murcia : F. Hernández, 2004) Coppens, A.G.; Salmon, I.; Heizman, C.W.; Poncelet, L.
The stria vascularis in the cochlea and the dark-cell areas in the vestibular endorgans are structures involved in the production of endolymphatic fluid. This study investigated the dark-cell areas in the vestibular endorgans of the dog by classical staining and by immunohistochemistry (anti-Na,K-ATPase ß2 isoform, anti-cytokeratins (against cytokeratins 5 and 8), antivimentin and anti-S100A6) from birth to 110 postnatal days. Using classical staining, it was not possible to discriminate dark cells from other epithelial cells lining the vestibular endolymphatic spaces. From birth, the Na,K-ATPase ß2 isoform was expressed in the lateral and basal cell membranes of a subset of cells located in the utricular wall, at the base of the cristae ampullaris and was identified as dark cells. From birth, anticytokeratins labelled all the cells forming the epithelial lining, including the dark cells, while anti-vimentin labelled the underlying mesenchymal cells. From postnatal day 10, anti-S100A6 labelled subepithelial cells exclusively located underneath the dark-cell areas and were identified as vestibular melanocyte-like cells. From birth, Fontana staining evidenced fine melanin granules in the subepithelial layer. The amount of melanin granules increased during the first month. Melanin distribution was closely associated with the region where S100A6-positive cells were located. The cell-specific antigen expression in the dog darkcell areas was clearly comparable to that of the dog stria vascularis previously described. The present investigation also suggested an earlier histological and immunohistological maturity in the dark-cell areas than in the stria vascularis of dogs. This preliminary morphological description of the normal dark-cell areas in dogs by means of immunomarkers may be instrumental in studying pathological processes involving the fluid-secreting structures in vestibular endorgans.
Open Access
Detection and measurement of alpha-amylase in canine saliva and changes after an experimentally induced sympathetic activation
(2017-08-22) Contreras Aguilar, María Dolores; Tecles Vicente, Fernando; Martínez Subiela, Silvia; Escribano Tortosa, Damián; Bernal Gambín, Luis Jesús; Cerón Madrigal, José Joaquín; Medicina y Cirugía Animal; Department of Food and Animal Science, School of Veterinary Medicine, Universitat Autònoma de Barcelona
Background: Salivary alpha-amylase (sAA) is considered a biomarker of sympathetic activation in humans, but there is controversy regarding the existence of sAA in dogs. The hypothesis of this study was that sAA exists in dogs and it could change in situations of sympathetic stimulation. Therefore, the aims of this study were: 1) to demonstrate the presence of alpha-amylase in saliva of dogs by Western-Blot, 2) to validate an spectrophotometric method for the measurement of sAA activity and 3) to evaluate the possible changes in sAA activity after the induction of an ejaculation in dogs which is known to produce a sympathetic activation. Results: Western-Blot demonstrated a band in dog saliva specimens between 60 kDa and 50 kDa, similar to purified sAA. The spectrophotometric assay validated showed an adequate inter- and intra-assay precision, and a high correlation coefficient (r = 0.999) in the linearity under dilution study. sAA median activity significantly increased just after ejaculation compared with just before the ejaculation (2.06-fold, P =0.005). Conclusions: This study demonstrated the existence of alpha-amylase in saliva of dogs and that this enzyme can be measured by a spectrophotometric assay. In addition, results showed that sAA increase after a sympathetic activation and could be potentially used as non-invasive biomarker of sympathetic activity in this species.
Open Access
Detection of transforming growth factor-a and epidermal growth factor receptor mRNA and immunohistochemical localization of the corresponding proteins in the canine uterus during the estrous cycle
(Murcia : F. Hernández, 2005) Tamada, H.; Tominaga, M.; Kida, K.; Kawate, N.; Inaba, T.; Matsuyama, S.; Sawada, T.
Uterine expression of the epidermal growth factor (EGF) family of growth factors has not been studied in the dog. The present study looks at the presence of mRNA transcripts and immunohistochemical localization for transforming growth factor-a (TGF-a), which is the potent EGF family member, and for EGF receptor (EGF-R) in the canine uterus during the estrous cycle. The reverse transcriptase-polymerase chain reaction together with sequencing of the products confirmed the presence of their mRNA transcripts in the endometrium throughout the estrous cycle. Immunohistochemical analysis found clear positive staining for TGF-a and EGF-R in the luminal and glandular epithelia at proestrus and estrus. Immunoreactivity decreased at the early stage of diestrus. In the mid stage of diestrus, clear staining for TGF-a was again found in the glands of the luminal region, and staining for EGF-R was observed in all glands. Very little staining was seen at anestrus for either TGF-a or EGF-R. These results suggest that TGF-a expressed in the uterus may be involved in regulating growth, differentiation and regression in the endometrial epithelial cells during the estrous cycle in the dog.
Open Access
Diagnóstico y factores de riesgo de giardiosis canina
(Universidad de Murcia, Servicio de Publicaciones, 2025) Rodríguez Arce, Elena; Bernal Catalán, Ana; Del Río Alonso, Laura; Departamentos
Giardia duodenalis es un parásito protozoario flagelado responsable de una de las infecciones parasitarias intestinales más frecuente en perros. La mayoría de casos son asintomáticos, pero resulta relevante para la salud pública debido a su potencial zoonótico. En algunos casos, como los colectivos caninos, el diagnostico directo es una alternativa más asequible al test de antígenos. Este estudio busca establecer la sensibilidad del diagnóstico en fresco a dos tiempos tras la toma de muestras. Además, busca determinar los principales factores de riesgo relacionados con la infección y la efectividad de los tratamientos previos. Para ello, se observó la presencia de giardiosis en muestras fecales de un total de 37 perros del Centro de Zoonosis del Ayuntamiento de Murcia, empleando el diagnóstico directo de muestras en fresco en el momento de recolección y 1-2 horas tras la recolección, comparando el resultado con el test de antígenos. En las muestras en fresco, se observó una prevalencia del 51,35% a tiempo 0, mientras que a tiempo 1, la prevalencia fue menor siendo de un 37,84%. La sensibilidad del diagnóstico en fresco se determinó empleando el test de antígenos como gold standard, obteniendo una sensibilidad del 72%. Se realizó un análisis descrip-tivo de los datos y se estudiaron los factores de riesgo y las asociaciones entre ellos. Se realizó un análisis de correspondencias múltiples (MCA) para observar la relación entre las diferentes variables. En conclusión, este estudio demuestra la alta sensibilidad del diagnóstico directo empleando muestras en fresco. Además, se establece la edad como principal factor de riesgo biótico, mientras que el tiempo de estancia en el centro es el principal factor de riesgo abiótico. Es importante destacar el efecto limitado de los tratamien-tos en la prevención de recidivas, lo que tiene implicaciones significativas en el manejo de le enfermedad en colectivos caninos.
Open Access
Distribution of bile acid receptor TGR5 in the gastrointestinal tract of dogs
(Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Giaretta, Paula R.; Suchodolski, Jan S.; Blick, Anna K.; Steiner, Joerg M.; Lidbury, Jonathan A.; Rech, Raquel R.
Takeda-G-protein-receptor-5 (TGR5) is a receptor for bile acids and its expression has been described in a variety of tissues and species. Characterization of TGR5 distribution and function has been investigated in drug discovery for the treatment of metabolic diseases in humans. Because dogs are one of the species used in biomedical research and share some similarities with human gastrointestinal diseases, the objective of this study was to characterize the distribution of TGR5 receptor in the canine species. This study characterizes the distribution of TGR5 receptor in the gastrointestinal tract, liver, gallbladder, and pancreas of 8 dogs. The distribution of TGR5 antigen and mRNA expression was investigated using immunohistochemistry and RNA in situ hybridization, respectively. TGR5 immunolabeling was located in the cell membrane or in the cell membrane and cytoplasm. TGR5 immunolabeling was broadly distributed in macrophages, endothelial cells, ganglion cells, and leiomyocytes throughout all the examined tissues. Epithelial cells from tongue, stomach to rectum, as well as from gallbladder, biliary and pancreatic ducts demonstrated TGR5 immunolabeling. In endocrine cells, TGR5 immunolabeling was observed in intestinal enteroendocrine cells and islets of Langerhans in the pancreas. The hepatocytes had a unique pattern of immunolabeling located on the canalicular surface of the cell membrane. TGR5 mRNA expression was located mainly in the nucleus and the only negative cells throughout all examined tissues were striated muscle from tongue and esophagus, muscularis mucosae, esophageal glands, and hepatic sinusoids. These findings indicate that the bile acid receptor TGR5 is ubiquitously distributed in the canine gastrointestinal tract.
Open Access
Ecocardiografía doppler pulsado y tisular en perros con enfermedad mitral degenerativa crónica estadio C estable
(Facultad de Veterinaria y el Servicio de Publicaciones de la Universidad de Murcia, 2023) García Sánchez, Vicente; Fernández del Palacio, Mª Josefa
La enfermedad mitral degenerativa crónica (EMCD) es la valvulopatía adquirida más frecuente en perros de raza pequeña y edad avanzada que es responsable de insuficiencia cardiaca congestiva. La ecocardiografía es el método de elección para efectuar el diagnóstico, clasificar a los pacientes y monitorizar el tratamiento. El objetivo de este estudio es examinar la utilidad del Doppler pulsado del flujo transmitral y Doppler tisular para evaluar la funcionalidad cardiaca en perros con enfermedad mitral degenerativa crónica en estadio C. Materia-les y métodos: Se incluyeron en el estudio 5 perros con EMDC en estadio C y 5 perros sanos de edades y pesos similares. Se seleccionó de los historial clínico de cada perro los datos de la reseña y siguientes parámetros ecocardiográficos: ratio AI/Ao, velocidades flujo transmitral (E, A y ratio E/A), velocidades del anillo mitral (lateral y medial) y radial en eje corto mediante Doppler tisular (S ́, E ́ y A ́), ratio E/E ́ y parámetros de fun-cionalidad del ventrículo derecho (excursión sistólica del plano del anillo tricúspide-TAPSE-, y Doppler tisular del anillo tricúspide lateral (S ́, E ́ y A ́)). Resultados: once de los diecinueve parámetros evaluados resultaron diferentes de forma significativa entre animales sanos y enfermos: elevación ratio AI/Ao, Vmax E, ratio E/A, E ́ en las tres regiones, S ́ (lateral y medial), ratio E/E ́, E ́ y S ́ del ventrículo derecho. Conclusiones: la ele-vación de los parámetros del Doppler tisular indica que la funcionalidad tanto del ventrículo izquierdo como derecho esta mantenida en perros con EMCD estadio C estable. Estos perros presentan presiones de llenado del ventrículo izquierdo muy elevadas (Vmax E; ratio E/A).
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Effects of Orchidectomy in Selective Biochemical Analytes in Beagle Dogs
(Blackwell Verlag GmbH, 2011-03-01) Tvarijonaviciute, Asta; Martínez Subiela, Silvia; Carrillo Sánchez, J. D.; Tecles Vicente, Fernando; Cerón Madrigal, José Joaquín; Medicina y Cirugía Animal; Facultades de la UMU::Facultad de Veterinaria
El objetivo de este estudio fue evaluar los posibles efectos de la orquiectomía y los cambios hormonales asociados sobre las concentraciones circulantes de proteínas de fase aguda (APPs) (CRP – proteína C reactiva; Hp – haptoglobina; Cp – ceruloplasmina), adiponectina e IGF-1 en perros. Para ello, un total de cinco perros adultos de raza Beagle fueron sometidos a orquiectomía. Se tomaron muestras de sangre antes de la castración, durante seis días consecutivos y en las semanas 2, 4, 8 y 12 tras la cirugía. Se mantuvo un régimen dietético adecuado para conservar estable el peso corporal de los perros. Las concentraciones de APPs aumentaron significativamente en los días 2–3 para la CRP y en los días 2–7 para la Hp y la Cp. En los días 3–4 tras la castración, los niveles de adiponectina fueron significativamente inferiores a los previos a la cirugía (p < 0,05 y < 0,01, respectivamente). Tras este cambio inicial, la adiponectina no mostró alteraciones significativas durante los 3 meses posteriores. Las concentraciones séricas de IGF-1 disminuyeron significativamente en los días 2–5 después de la castración. Además, en las semanas 8 y 12 los niveles séricos de IGF-1 fueron significativamente menores (p < 0,001 y < 0,01 respectivamente) en comparación con los valores previos a la cirugía. En conclusión, la orquiectomía indujo un proceso inflamatorio a corto plazo que se asoció con un aumento de los niveles séricos de APPs y una disminución de los niveles de IGF-1 y adiponectina. Sin embargo, la orquiectomía no produjo cambios a largo plazo en las concentraciones circulantes de APPs ni de adiponectina. Aunque se registró una disminución de los niveles de IGF-1 dos meses después de la cirugía, posiblemente como consecuencia de la reducción asociada de los niveles de andrógenos o de la restricción alimentaria.
Open Access
Epithelial mesenchymal transition in the progression of renal disease in dogs
(F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 2014) Benali, S.L.; Lees, G.E.; Castagnaro, M.; Aresu, L.
Chronic kidney disease (CKD) in dogs is the final common pathway resulting from persistent renal injury and is characterized by progressive tubulointerstitial damage (TID). Pathogenesis of CKD is divided into an initial inflammatory phase with a predominantly mononuclear infiltrate followed by a fibrotic phase with increased numbers of fibroblasts and extracellular matrix deposition that causes a progressive reduction of functional parenchyma. Proteinuria is a common manifestation of renal diseases in dogs, and its role in the pathogenesis of CKD is still uncertain. Nevertheless, the degree of proteinuria in dogs correlates with TID progression. Increased protein filtration may have direct effects on tubular epithelial cells (TECs) that induce them to express the major histocompatibility complex type II, and thereby contribute to lymphocyte recruitment. Thus, an active pro-inflammatory role is proposed for TECs in TID progression. Moreover TECs are believed to actively participate in the mechanisms of renal fibrosis. Epithelial-Mesenchymal-Transition (EMT) of TECs in canine TID has been studied in the last decade. Downregulation of adhesion molecules and loss of epithelial markers in TECs directly correlate with the severity of TID and with de novo expression of mesenchymal markers. Tubular basement membrane (TBM) disruption is an early EMT event. Increased activity of Matrix Metalloproteinase-2 and its co-localization with TBM splitting suggests an active role for the enzyme in inducing EMT. Processes occurring in canine CKD share many similarities with its human counterpart, making the dog a good model in which to examine the mechanisms of TID progression.
Open Access
Expression of Ccdc85C, a causative protein for murine hydrocephalus, in the mammary gland tumors of dogs
(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Tanaka, Natsuki; Izawa, Takeshi; Takenaka, Shigeo; Akiyoshi, Hideo; Yamate, Jyoji; Kuwamura, Mitsuru
Coiled-coil domain containing 85c (Ccdc85c) is a causative gene for hemorrhagic hydrocephalus mouse which shows hydrocephalus with frequent brain hemorrhage and formation of subcortical band heterotopia. A previous study revealed that Ccdc85C protein is expressed in the systemic simple epithelial cells with proliferative activity in rats and suggested that Ccdc85C expression may be related to the cell proliferation of simple epithelial cells. To reveal the roles of Ccdc85C in the proliferative lesion, we examined the expression patterns of Ccdc85C in the mammary gland tumor of dogs, a common representative tumor derived from simple epithelial cells. In canine mammary gland tumors, Ccdc85C was expressed at the apical junctions of the luminal epithelial cells. Ccdc85C was also distributed throughout the entire cytoplasm of the myoepithelial cells. Ccdc85C expression was observed at the epithelial cells with luminal structures, but was not observed at the epithelial cells forming sheet growth pattern without luminal structure. In carcinomas, Ccdc85C expression in mammary tumor tissue tended to be weaker than that in surrounding normal mammary gland tissue. Ccdc85C is known to cause neurological diseases such as hydrocephalus, and subcortical heterotopia, and the present study is the first to demonstrate Ccdc85C expression in canine mammary tumors and a relationship between Ccdc85C expression and tumor malignancy
Open Access
Expression of platelet-derived growth factor and its receptors in spontaneous canine hemangiosarcoma and cutaneous hemangioma
(F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 2012) Abou Asa, S.; Murai, A.; Murakami, M.; Hoshino, Y.; Mori, T.; Maruo, K.; Khater, A.; El-sawak, A.; Abd el-Aziz, E.; Yanai, T.; Sakai, H.
Hemangiosarcoma (HSA) is a malignant neoplasia of vascular endothelial cells (ECs). Our previous report on the expression of vascular endothelial growth factor, basic fibroblast growth factor, and their receptors in canine HSA suggested an autocrine/paracrine mechanism of tumor growth. However, the influence of other angiogenic growth factors in canine HSA was not elucidated; therefore, the expression of platelet-derived growth factor (PDGF) and its receptors was investigated by immunohistochemical analysis. Forty-six canine HSAs and 21 canine cutaneous hemangiomas (HAs) were analyzed. For immunohisto-chemistry, anti-PDGF-BB, anti-PDGFR-α, and anti-PDGFR-ß antibodies were utilized as primary antibodies. Immunoreactivities were scored as strongly positive (>25% positive neoplastic cells), weakly positive (1-25% positive neoplastic cells), and negative if not staining at all. In cutaneous HA, 33.3% and 57.1% of cases were strongly and weakly positive, respectively, and 43.5% and 13.0% of HSAs were strongly and weakly positive for PDGF-BB, respectively. Moreover, 38.1% and 28.6% of cutaneous HAs cases were strongly and weakly positive, respectively, and 23.9% and 4.3% of HSAs cases were strongly and weakly positive, respectively, for PDGFR-α. Thirty-five HSAs cases (76.1%) were strongly positive, and the remaining 11 (23.9%) were weakly positive for PDGFR-ß. In contrast, 18 (72.0%) cutaneous HAs were negative, and only 3 cases (12.0%) were weakly positive, for PDGFR-ß. The proportion of strongly positive cases of HSAs was significantly higher than that of cutaneous HA for PDGFR-ß (P<0.01), while PDGFR-α was highly expressed in cutaneous HA and may be related to pathogenesis of cutaneous HA. Therefore, PDGFR-ß may be associated with the malignant nature of canine HSA
Open Access
Ezrin and moesin expression in canine and feline osteosarcoma
(Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Hlavaty, Juraj; Wolfesberger, Birgitt; Hauck, Marlene; Obermayer Pietsch, Barbara; Fuchs Baumgartinger, Andrea; Miller, Ingrid; Walter, Ingrid
Biological features of canine osteosarcomas (OS) differ markedly from those found in feline and resemble more human osteosarcomas, in particular for their high rate of metastasis and poor prognosis. Ezrin, radixin and moesin are members of the ERM protein family and link the actin cytoskeleton with the cell membrane. Ezrin and moesin have been shown to be of prognostic significance in tumor progression due to their role in the metastatic process. The objective of this study was to analyze ezrin and moesin protein expression in a series of dog (n=16) and cat (n=8) osteosarcoma samples using immunohistochemistry and western blot techniques. We found that cat OS have a higher moesin expression compared to dog OS, however, the active phosphorylated forms of moesin and ezrin Tyr353 were more abundant in the dog samples. A statistically significant difference was found for the low and high immunohistochemical scores of ezrin and pan-phosphoERM proteins between cat and dog. Although phosphoezrin Thr567 was higher in feline OS, the membranous localization in dog OS samples indicates the presence of the biologically active form. Therefore, the observed differences in phosphorylated forms of ezrin and moesin status should be further studied to demonstrate if they are relevant for different biological behavior between dog and cat OS.
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Identification of changes in serum analytes and possible metabolic pathways associated with canine obesity-related metabolic dysfunction
(Elsevier, 2019-02) Tvarijonaviciute, Asta; Barić Rafaj, R.; Horvatic, A.; Muñoz Prieto, A.; Guillermin, N.; Tumpa, A.; Cerón, J. J.; Martínez Subiela, S.; Mrljak, V.; Medicina y Cirugía Animal
The main objective of this study was to identify analytes that could change and that could help to clarify the metabolic and physiopathological changes related to canine obesity-related metabolic dysfunction (ORMD). For this, serum from 35 overweight/obese dogs, with and without ORMD, was submitted to a comprehensive panel of biochemistry analysis, a gel-free tandem mass tag isobaric label-based proteomic analysis, and, finally, selected proteins were used as a starting point for creating a protein interaction network. Dogs with ORMD showed significantly higher serum concentrations of alanine aminotransferase (ALT), alkaline phosphatase (ALP), Ca, total proteins, albumin, total cholesterol, triglycerides, glucose, and butyrylcholinesterase (BChE) activity in comparison with dogs without ORMD. Proteomic analysis revealed that 23 proteins related to lipid metabolism, the complement factor system, cellular adhesion and functionality, inflammation, and coagulation were altered in dogs with ORMD. Finally, the obtained protein interaction network highlighted that the central term of this network was the negative regulation of the immune response. These data suggest that canine ORMD is associated with changes in analytes that reflect altered lipid metabolism, and liver and immune function impairment and suggests the potential for a prothrombotic state and lung function alterations.
Open Access
Immunohistochemical evaluation of intermediate filament nestin in dog hair follicles
(Murcia : F. Hernández, 2008) Mercati, F.; Pascucci, L.; Gargiulo, A.M.; Dall’Aglio, C.; Ceccarelli, P.
Hair follicles (HFs) are self-renewing structures that reconstitute themselves through the hair cycle. They maintain reservoirs of stem cells (SC) that are thought to reside in the bulge area, a region localized in the lowermost permanent portion of HFs. In mice and humans, HF bulge cells express nestin and present stem features as pluripotency. Nestin is a class VI intermediate filament protein; it was first described as a specific marker of CNS stem cells, but recent studies suggest that it may represent a more general stem cell marker (Wiese et al., 2004; Hoffman, 2006). Bulge cell characteristics have mainly been studied in mice and humans, but recently, a bulge-like region was identified also in dog HFs (Pascucci et al., 2006). In this work we investigate the presence and localization of nestin in dog HFs with the aim of evaluating its expression and to correlate it with the location of the bulge-like region. Immunostaining of skin samples collected from healthy dogs was performed by using a rabbit anti-nestin polyclonal antibody. The presence of a population of immunoreactive cells was revealed in the hair follicle middle region, at the arrector pili muscle insertion level. An immunohistochemical signal was detected only in primary hair follicles throughout the hair cycle. These observations led us to conclude that nestin positive cells are located in the bulge-like region of dog HFs and strengthen our hypothesis regarding the correlation between this region and the dog HF stem compartment.