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Browsing Artículos by browse.metadata.contributordepartment "Biología Celular e Histología"
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- PublicationOpen Access17alpha-ethynylestradiol prevents the natural male-to-female sex change in gilthead seabream (Sparus aurata L.)(Springer Nature, 2020-11) García Hernández, M.P.; Cabas, I.; Rodenas, M.C.; Arizcun, M.; Chaves-Pozo, E.; Power, D.M.; García Ayala, Alfonsa; Biología Celular e Histología
- PublicationOpen Access17α-Ethynylestradiol alters the peritoneal immune response of gilthead seabream(Elsevier, 2017) Gómez González, Nuria Esther; Cabas, Isabel; Rodenas, María del Carmen; Arizcun, Marta; Mulero Méndez, Victoriano Francisco; García Ayala, Alfonsa; Biología Celular e Histología17α-Ethynylestradiol (EE2), a synthetic estrogen used in most oral contraceptives pills and hormone replacement therapies, is found in many water bodies, where it can modulate the fish immune response. EE2 acts as an endocrine disruptor in gilthead seabream, Sparus aurata L., a marine teleost fish of great economic value in Mediterranean aquaculture, as it induces hepatic vitellogenin gene (vtg) expression. Moreover, EE2 also alters the capacity of gilthead seabream to appropriately respond to infection although it does not behave as an immunosuppressor. Nevertheless, these previous studies have mainly focused on the head kidney leukocytes and no information exists on peritoneal leukocytes, including mast cells. In the present work, juvenile gilthead seabream fish were fed a pellet diet supplemented with EE2 for 76 days and intraperitoneally injected with hemocyanin plus imject alum adjuvant at the end of EE2 treatment and 92 days later, and the peritoneal immune response was analyzed. EE2 supplementation induced vtg expression but returned to basal levels by 3 months post-treatment. Interestingly, gilthead seabream peritoneal leukocytes express the genes encoding for the nuclear estrogen receptor α and the G protein-coupled estrogen receptor 1 and the dietary intake of EE2 induced these expression. Moreover, EE2 induced an inflammatory response in the peritoneal cavity in unvaccinated fish, which was largely maintained for several months after the cessation of the treatment. However, the impact of EE2 in vaccinated fish was rather minor and transient. Taken together, the study provides fresh information about endocrine immune disruption, focusing on peritoneal leukocytes.
- PublicationOpen AccessA simple format feed to test the acceptability of ingredients for common octopus (Octopus vulgaris Cuvier, 1797)(Wiley, 2015-03-10) Sánchez Morillo-Velarde, Mª Piedad; Cerezo Valverde, Jesús; García García, Benjamín; Biología Celular e Histología
- PublicationOpen AccessAluminum is a powerful adjuvant in teleost fish despite failing to induce interleukin-1β release(2018-08) Angosto, Diego; López-Muñoz, Azucena; García-Alcázar, Alicia; Meseguer, José; Sepulcre Cortés, María Pilar; Biología Celular e Histología
- PublicationOpen AccessAn infrared investigation in relation with chitin and chitosan characterization(Elsevier, 2001-01-12) Brugnerotto, J.; Lizardi, J.; Goycoolea Valencia, Francisco Martín; Argüelles Monal, Waldo; Desbrières, J.; Rinaudo, Marguerite; Biología Celular e HistologíaThe use of infrared spectroscopy for characterization of the composition of chitin and chitosan covering the entire range of degree of acetylation (DA) and a wide variety of raw materials is examined further. The ratio of absorbance bands selected was calibrated using 1H liquid and 13C CP-MAS solid-state NMR as absolute techniques. IR spectra of the structural units of these polymers validated the choice of baselines and characteristic bands. The bands at 1650 and 1320 cm-1 were chosen to measure the DA. As internal reference, the intensities at 3450 and 1420 cm-1 were evaluated. The absorption band ratios involving the reference at 3450 cm-1 had poorer fit. The absorption ratio A1320/A1420 shows superior agreement between the absolute and estimated DA-values (DA% = 31.92A1320/A1420 - 12.20; r = 0.990)
- PublicationOpen AccessAnalysis of ZP1 gene reveals differences in zona pellucida composition in carnivores(2017) Moros-Nicolás, C.; Leza, A.; Chevret, P.; Guillén-Martínez, A.; González-Brusi, L.; López-Béjar, M.; Ballesta, J.; Avilés, M.; Izquierdo-Rico, M.J.; Biología Celular e Histología
- PublicationOpen AccessAptamer-Target-gold nanoparticle conjugates for the quantification of fumonisin B1.(MDPI, 2021-01-08) Vicente A Mirón-Mérida; Yadira González-Espinosa; Mar Collado-González; Yun Yun Gong; Yuan Gong; Francisco M Goycoolea; Biología Celular e Histología; Facultades de la UMUFumonisin B1 (FB1), a mycotoxin classified as group 2B hazard, is of high importance due to its abundance and occurrence in varied crops. Conventional methods for detection are sensitive and selective; however, they also convey disadvantages such as long assay times, expensive equipment and instrumentation, complex procedures, sample pretreatment and unfeasibility for on-site analysis. Therefore, there is a need for quick, simple and affordable quantification methods. On that note, aptamers (ssDNA) are a good alternative for designing specific and sensitive biosensing techniques. In this work, the assessment of the performance of two aptamers (40 and 96 nt) on the colorimetric quantification of FB1 was determined by conducting an aptamer–target incubation step, followed by the addition of gold nanoparticles (AuNPs) and NaCl. Although MgCl2 and Tris-HCl were, respectively, essential for aptamer 96 and 40 nt, the latter was not specific for FB1. Alternatively, the formation of Aptamer (96 nt)–FB1–AuNP conjugates in MgCl2 exhibited stabilization to NaClinduced aggregation at increasing FB1 concentrations. The application of asymmetric flow field-flow fractionation (AF4) allowed their size separation and characterization by a ultidetection system (UV-VIS, MALS and DLS online), with a reduction in the limit of detection from 0.002 g/mL to 56 fg/mL.
- PublicationOpen AccessAssessing chemical toxicity of ionic liquids on Vibrio fischeri: Correlation with structure and composition(Pergamon – Elsevier Science Ltd, 2016-04-11) Montalbán, Mercedes G; Hidalgo, Juana M; Collado-González, Mar; Díaz Baños, Francisco Guillermo; Víllora, Gloria; Biología Celular e HistologíaOne of the most important properties of the ionic liquids (ILs) is their non-volatility. However, they are wide soluble in water. For this reason, they can be released to aquatic ecosystems and to contribute to water pollution. Nevertheless, toxicological data related to ionic liquids is scarce in literature because of the great number of possible ionic liquids synthesized. The present work reports the toxicity of twenty-nine imidazolium-, pyridinium- and ammonium-based ionic liquids towards the luminescent bacteria Vibrio fischeri. Some of the effects analyzed on the toxicity have been the type of anion, the length of the alkyl chain of the cation, the cation core and the presence of a functionalized side chain in the cation. These results have showed that the main influence on the toxicity of the ILs is the alkyl chain length. A Quantitative Structure-Activity Relationships (QSAR) method has been used to validate our results obtaining a very good agreement.
- PublicationOpen AccessAssociative phenomena in galactomannan-deacetylated xanthan systems(Elsevier, 2001-09-30) Goycoolea Valencia, Francisco Martín; Milas, Michel; Rinaudo, Marguerite; Biología Celular e HistologíaThe interaction between mesquite seed galactomannan (MSG; D-mannose to D-galactose ratio (M/G) ~1.1) and deacetylated xanthan (DX) in 5 mM NaCl leading to synergistic gel formation at 25 °C was investigated and compared with the far more studied system made of xanthan and locust bean gum (LBG; M/G ~3.5). Rheology and differential scanning calorimetry were used to measure temperatures of gel formation and transition enthalpy as a function of polymer composition, while circular dichroism was used to probe the conformation of DX in the LBG– DX system. MSG and DX associate at 25 °C with a well defined stoichiometry of 0.6:1.0 (w/w) at low ionic strength favouring the disordered coil state of DX. When LBG was used in place of MSG in water or 5 mM NaCl, two types of mechanisms of interpolymeric association are envisaged.
PublicationOpen AccessBeneficial roles of feed additives as immunostimulants in aquaculture: A review(2018) Dawood, Mahmoud A.O.; Koshio, Shunsuke; Esteban Abad, María Ángeles; Biología Celular e HistologíaThe use of antibiotics in the aquafeeds to mitigate infectious diseases or to boost growth performance is commonly practiced. Recently, the prophylactic use of antibiotics and chemotherapies have been criticized which eventually led to their ban in aquaculture by law on many countries. In view of the current restrictions on antibiotic use, there is a critical need to evaluate other possible alternatives. One potential substitute for antibiotics in aquafeeds is the use of functional feed additives. The present review is a comprehensive and an updated compilation of the available works on growth promoters and novel feed supplements, their examples, modes of action and useful applications for improving aquafeeds. It highlights probiotics, prebiotics, synbiotics, immunostimulants, organic acids, nucleotides and medicinal herbs. Apart from boosting aquafeeds and safeguarding general health of aquatic animals, some of these substances have been found to possess beneficial immunostimulant and anti-stress relieving properties. Furthermore, the use of these natural substances increases the consumer confidence of farmed fish. Due care has been taken to cover the ongoing trends and recent advances with a perspective vision and their holistic usages and beneficial applications in aquatic animals’ systems. The current study also shed light regarding the management and production aspects of aquatic animals which will enlighten farmers and producers for better economic growth.- PublicationOpen AccessBiocompatibility of new pulp-capping materials NeoMTA Plus, MTA Repair HP and Biodentine on human dental pulp stem cells(Elsevier Science, 2017-11-01) Tomás-Catalá, Christopher J.; Collado-González, Mar ; García-Bernal, David; Oñate-Sánchez, Ricardo E.; Forner, Leopoldo ; Llena, Carmen ; Lozano, Adrián ; Moraleda, José M.; Rodríguez-Lozano, Francisco J.; Biología Celular e Histología; Facultades de la UMUIntroduction: The aim of the present study was to evaluate the in vitro cytotoxicity of MTA Repair HP, Neo-MTA Plus, and Biodentine, new bioactive materials used for dental pulp capping, on human dental pulp stem cells (hDPSCs). Methods: Biological testing was carried out in vitro on hDPSCs. Cell viability and cell migration assays were performed using eluates of each capping material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. The chemical composition of the pulp-capping materials was determined by energy-dispersive X-ray and eluates were analyzed by inductively coupled plasma-mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results: Cell viability was moderate after 24 and 48 hours in the presence of MTA Repair HP and NeoMTA Plus, whereas at 48 and 72 hours, Biodentine showed higher rates of cell viability than MTA Repair HP and NeoMTA Plus (P < .001). A cell migration assay revealed adequate cell migration rates for MTA Repair HP and NeoMTA Plus, both similar to the control group rates, meanwhile the highest cell migration rate was observed in the presence of Biodentine (P < .001). Scanning electron microscope studies showed a high degree of cell proliferation and adhesion on Biodentine disks but moderate rates on MTA Repair HP and NeoMTA Plus disks. Energydispersive X-ray pointed to similar weight percentages of C, O, and Ca in all 3 materials, whereas other elements such as Al, Si, and S were also found. Conclusions: The new pulp-capping materials MTA Repair HP, NeoMTA Plus, and Biodentine showed a suitable degree of cytocompatibility with hDPSCs, and good cell migration rates, although Biodentine showed higher rates of proliferation time-dependent.
- PublicationOpen AccessBoar sperm motility is modulated by CCK at a low concentration of bicarbonate under capacitation conditions(2023) Moros Nicolás, Carla; López-Úbeda, R.; Luongo, C.; Sòria-Monzó, P.; Abril-Sánchez, S.; Grudzinska, P.; Avilés, M.; Izquierdo-Rico, M.J.; García-Vázquez, F.A.; Biología Celular e Histología
- PublicationOpen AccessCarbamazepine, cadmium chloride and polybrominated diphenyl ether-47, synergistically modulate the expression of antioxidants and cell cycle biomarkers, in the marine fish cell line SAF-1(Elsevier, 2019-11-21) Espinosa Ruiz, Cristóbal; Manuguerra, Simona; Curcuraci, Eleonora; Santulli, Andrea; Messina, Concetta M.; Biología Celular e HistologíaA wide range of contaminants, industrial by-products, plastics, and pharmaceutics belonging to various categories, have been found in sea water. Although these compounds are detected at concentrations that might be considered as sub-lethal, under certain conditions they could act synergistically producing unexpected effects in term of toxicity or perturbation of biochemical markers leading to standard pathway. In this study, the Sparus aurata fibroblast cell line SAF-1, was exposed to increasing concentrations of carbamazepine (CBZ), polybrominated diphenyl ether 47 (BDE-47) and cadmium chloride (CdCl2) until 72 h, to evaluate the cytotoxicity and the expression of genes related to antioxidant defense, cell cycle and energetic balance. In general, both vitality and gene expression were affected by the exposure to the different toxicants, in terms of antioxidant defense and cell cycle control, showing the most significant effects in cells exposed to the mixture of the three compounds, respect to the single compounds separately. The synergic effect of the compounds on the analyzed biomarkers, underlie the potential negative impact of the contaminants on health of marine organisms.
- PublicationOpen AccessChanges in lipid composition of different tissues of common octopus (Octopus vulgaris) during short-term starvation(Wiley, 2013-07-09) Sánchez Morillo-Velarde, Mª Piedad; Cerezo Valverde, Jesús; Serra Llinares, Rosa M.; García García, Benjamín; Biología Celular e HistologíaIn this work, we study the variations in concentration (mg 100 g−1 dry weight) and total content (mg individual−1) of different lipid classes in muscle and the digestive gland of Octopus vulgaris during an 8-day starvation period. In all the samples analysed, polar lipids (PL) predominated in muscle (267.3–337.2 mg 100 g−1) compared with neutral lipids (66.9–104.7 mg 100 g−1). A significant positive tendency was observed in muscle for the content and concentration of monoglycerides as a consequence of starvation (P < 0.05). Furthermore, a higher cholesterol (CHO) content was detected in this tissue after the fourth day of starvation compared with the fed animals (P < 0.05). Neutral lipids predominated in the digestive gland (12 958–14 151 mg 100 g−1) compared with PL (3157–6517 mg 100 g−1), with triglycerides, free fatty acids and monoglycerides being the major lipid classes. The concentration of PL and monoglycerides showed a positive trend with starvation, while the triacylglycerol showed a negative tendency (P < 0.05). The results suggest that triglycerides and CHO are transported simultaneously from the digestive gland to the muscular tissues during starvation and the preferential use of PL and CHO during growth phases. It was estimated that lipids contributed 26% of the energy costs of the animals during starvation, mainly in the form of triglycerides from the digestive gland.
- PublicationOpen AccessCharacterisation of the interaction among oil-in-water nanocapsules and mucin(MDPI, 2020-07-28) Collado-González, Mar; Kaur, Gurmeet ; González-Espinosa, Yadira ; Brooks, Rebecca ; Goycoolea, Francisco M.; Biología Celular e HistologíaMucins are glycoproteins present in all mucosal surfaces and in secretions such as saliva. Mucins are involved in the mucoadhesion of nanodevices carrying bioactive molecules to their target sites in vivo. Oil-in-water nanocapsules (NCs) have been synthesised for carrying N,N0-(di-m-methylphenyl)urea (DMTU), a quorum-sensing inhibitor, to the oral cavity. DMTU-loaded NCs constitute an alternative for the treatment of plaque (bacterial biofilm). In this work, the stability of the NCs after their interaction with mucin is analysed. Mucin type III from Sigma-Aldrich has been used as the mucin model. Mucin and NCs were characterised by the multi-detection symmetrical flow field-flow fractionation technique (AF4). Dynamic light scattering (DLS) and z-potential analyses were carried out to characterise the interaction between mucin and NCs. According to the results, loading DMTU changes the conformation of the NC. It was also found that the synergistic interaction between mucin and NCs was favoured within a specific range of the mucin:NC ratio within the first 24 h. Studies on the release of DMTU in vitro and the microbial activity of such NCs are ongoing in our lab.
- PublicationOpen AccessChitosan as stabilizing agent for negatively charged nanoparticles(Elsevier, 2016-12-24) Collado-González, Mar; Montalbán, Mercedes G.; Peña-García, Jorge; Pérez-Sánchez, Horacio; Víllora, Gloria; Díaz Baños, F. Guillermo; Biología Celular e HistologíaChitosan is a biocompatible polysaccharide with positive Z potential which can stabilize negative charged nanoparticles. Silk fibroin nanoparticles and citrate gold nanoparticles, both with negative Z potential, but they form aggregates at physiological ionic strength. In this work, we study the behavior of chitosan in solution when the ionic strength of the medium is increased and how the concentration of chitosan and the proportion of the two components (chitosan and AuNP or SFN) significantly affect the stability and size of the nanocomposites formed. In addition to experimental measurements, molecular modeling were used to gain insight into how chitosan interacts with silk fibroin monomers, and to identify the main energetic interactions involved in the process. The optimum values for obtaining the smallest and most homogeneous stable nanocomposites were obtained and two different ways of organization through which chitosan may exert its stabilizing effect were suggested.
- PublicationOpen AccessChitosan-nanoparticles effects on mucosal immunity: a systematic review(0026-08-22) Mar Collado-González; M Ángeles Esteban; Biología Celular e Histología; Facultades de la UMUNanoparticles-based treatments is of utmost importance for aquaculture. In this scenario, chitosan-based nanoparticles have been proposed due to the properties of chitosan, which include mucoadhesiveness. Nevertheless, pivotal parameters of chitosan, such as degree of acetylation and molecular weight, are commonly underestimated in the available literature despite the influence they seem to have on the properties of chitosan-based nanoparticles. In this systematic review, the immunomodulator capacity of chitosan nanoparticles used as mucosal vaccines on teleost fish has been evaluated paying special attention to the chitosan properties. Four databases were used for literature search, yielding 486 documents, from which 14 meet the inclusion criteria. Only 21% of the available studies reported properly chitosan properties, which should be improved in future works to generate reproducible data as well as valuable information. To the best of our knowledge, this work objectively compares for the first time, by quantifying the mg of chitosan/g of fish applied in each study, the chitosan nanoparticle preparation and doses applied to fish, as well as the effects of the treatments applied on fish immune status.
- PublicationOpen AccessChitosan/albumin coating factorial optimization of alginate/dextran sulfate cores for oral delivery of insulin(Multidisciplinary Digital Publishing Institute (MDPI), 2023-03-14) Pessoa, Bruno ; Collado-González, Mar ; Sandri, Giuseppina ; Ribeiro, António ; Biología Celular e HistologíaThe design of nanoparticle formulations composed of biopolymers, that govern the physicochemical properties of orally delivered insulin, relies on improving insulin Stability and absorption through the intestinal mucosa while protecting it from harsh conditions in the gastrointestinal (GI) tract. Chitosan/polyethylene glycol (PEG) and albumin coating of alginate/dextran sulfate hydrogel cores are presented as a ultilayer complex protecting insulin within the nanoparticle. This study aims to optimize a anoparticle formulation by assessing the relationship between design parameters and experimental data using response surface methodology through a 3-factor 3-level optimization Box–Behnken design. While the selected independent variables were the concentrations of PEG, chitosan and albumin, the dependent variables were particle Size, polydispersity index (PDI), zeta potential, and insulin release. Experimental results showed a nanoparticle size ranging from 313 to 585 nm, with PDI from 0.17 to 0.39 and zeta potential ranging from 29 to 44 mV. Insulin bioactivity was maintained in simulated GI media with over 45% cumulative release after 180 min in a simulated intestinal medium. Based on the experimental responses and according to the criteria of desirability on the experimental region’s constraints, solutions of 0.03% PEG, 0.047% chitosan and 1.20% albumin provide an optimum nanoparticle formulation for insulin oral delivery.
- PublicationOpen AccessColostrum Features of Active and Recovered COVID-19 Patients Revealed Using Next-Generation Proteomics Technique, SWATH-MS(2023) Hernández-Caravaca, Ivan; Moros Nicolás, Carla; González-Brusi, Leopoldo; Romero de Ávila, Mª José; De Paco Matallana, Catalina; Pelegrín, Pablo; Castaño-Molina, Mª Angeles; Díaz-Meca, Lucía; Sánchez-Romero, Javier; Martínez-Alarcón, Laura; Avilés, Manuel; Izquierdo-Rico, Mª José; Biología Celular e HistologíaColostrum performs nutritional, anti-inflammatory and anti-infective functions and promotes immune system formation and organ development. The new coronavirus, SARS-CoV-2, has generated concerns about viral transmission through human milk, with a lack of evidence about human milk’s protective effects against the infection. This study aimed at analyzing presence of the virus and at identifying the protein expression profile of human colostrum in active and COVID-19- recovered patients. Colostrum samples were collected from women with COVID-19 (n = 3), women recently recovered from the infection (n = 4), and non-infected women (n = 5). The samples were analyzed by means of RT-qPCR to determine presence of the virus and using SWATH-MS for proteomic analysis. Proteomic results were then analyzed using bioinformatic methods. The viral tests were negative for SARS-CoV-2 in the colostrum from COVID-19 patients. The proteomic analysis identified 301 common proteins in all samples analyzed. Nineteen proteins were upregulated and 7 were downregulated in the COVID-19 group versus the control samples, whereas 18 were upregulated and 7 were downregulated when comparing the COVID-19 group to the recovered group. Eleven proteins were biomarkers of active COVID-19 infection. Ten were upregulated: ACTN1, CD36, FAM3B, GPRC5B, IGHA2, IGK, PLTP, RAC1, SDCBP and SERPINF1, and one was downregulated: PSAP. These proteins are mainly related to immunity, inflammatory response and protein transport. In conclusion, the results of this study suggest that colostrum is not a vehicle for mother-to-child SARS-CoV-2 transmission. Moreover, the colostrum’s proteome of active and recuperated patients indicate that it could provide immune benefits to infants.
- PublicationOpen AccessComparative analysis of the biological effects of the endodontic bioactive cements MTA Angelus, MTA Repair HP, and NeoMTA Plus on human dental pulp stem cells(Willey, 2017-09-11) Tomás Catalá, Christopher J.; Collado-González, María del Mar; García-Bernal, David; Oñate-Sánchez, Ricardo E.; Forner, Leopoldo ; Llena, Carmen; Lozano, Adrián ; Castelo-Baz, P.; Moraleda, José M.; Rodríguez-Lozano, Francisco J.; Biología Celular e HistologíaAim To evaluate the biological effects in vitro of MTAAngelus (MTA-Ang; Angelus, Londrina, PR, Brazil), MTA Repair HP (MTA-HP; Angelus) and NeoMTA Plus (NeoMTA-P; Avalon Biomed Inc, Bradenton, FL, USA) on human dental pulp stem cells (hDPSCs). Methodology Cell viability and cell migration assays were performed using eluates of each material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analysed by immunocytofluorescence and scanning electron microscopy, respectively. The chemical composition of the materials was determined by energy-dispersive X-ray (EDX), and eluates were analysed by inductively coupled plasma–mass spectrometry (ICP-MS). Statistical analysis was performed with the analysis of variance and Bonferroni or Tukey post-test (a < 0.05). Results Undiluted MTA-Ang, MTA-HP and NeoMTA-P displayed a significant increase in cell viability greater than that obtained using complete medium alone (control) (*P < 0.05; **P < 0.01; ***P < 0.001). Moreover, a cell migration assay revealed cell migration rates after incubation with extracts of MTA-Ang, MTA-HP and NeoMTA-P that were similar to levels obtained in the control group. In addition, stretched cytoskeletal F-actin fibres were detected in the cells treated with the three material extracts. SEM studies revealed a high degree of cell proliferation and attachment on all three materials. EDX analysis demonstrated similar weight percentages of C, O and Ca in all three materials, whilst other elements such as Al, Si and S were also found. Conclusions MTA-Ang, MTA-HP and NeoMTA-P were associated with biological effects on hDPSCs in terms of cell proliferation, morphology, migration and attachment.