Publication: SCREENING AND SELECTION OF LIPASES FOR THE ENZYMATIC PRODUCTION OF POLYGLYCEROL POLYRICINOLEATE
Authors
Bódalo, A. ; Bastida Rodríguez, Josefa ; Máximo Martín, María Fuensanta ; Montiel Morte, María Claudia ; Gómez, M. ; Ortega Requena, Salvadora
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DOI
https://doi.org/10.1016/j.bej.2009.05.004
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info:eu-repo/semantics/article
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Abstract
We have demonstrated, for the first time, that polyglylcerol polyricinoleate (PGPR) can be
synthesised using lipases as biocatalyst with very good results. Of the twenty one lipases screened
for their ability to catalyse PGPR production from a mixture of polyricinoleic acid and polyglycerol-3,
only twelve lipases were able to catalyse the reaction. All of them were from microbial sources
(bacteria and fungi) and were 1, 3-specific or "random" lipases. The selection procedure was based
not only on the enzymatic activity but also on economic criteria. Lipases from Mucor javanicus,
Rhizopus arrhizus and Rhizopus oryzae were finally chosen, and all three enzymes were
successfully immobilized by adsorption onto an anion exchange resin where they showed their
suitability to catalyse the synthesis of PGPR. This represents a promising starting point for
developing an industrial process for the green production of polyglycerol polyricinoleate.
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